Resection interventions in the treatment of hepatocellular cancer in a specialized surgery center

Background. The relevance of the treatment of hepatocellular cancer (HCC) is determined by a significant increase in the incidence rate and its high prevalence among primary malignant hepatic tumors.The aim of the study. To summarize the experience of providing specialized medical care to patients with hepatocellular cancer.Methods. We studied the direct results of treatment of patients with primary hepatic cancer treated at the Kuzbass Regional Hepatological Center for the period from January 2015 to August 2022. The materials for the study were medical records of patients with an established diagnosis of primary hepatic cancer, surgical records, results of pathohistologic examination. The exclusion criterion was diagnosed cholangiocellular carcinoma.Results. During the period from 2015 to 2022, 59 patients with primary hepatic cancer were treated at the Kuzbass Regional Hepatological Center. Among them, hepatocellular cancer was diagnosed in 48 cases, cholangiocellular cancer – in 11 cases; radical surgery was performed in 12 patients with hepatocellular cancer; fatal outcome was noted in 1 (2.1 %) patient, complications in the postoperative period developed in 5 (41.7 %) cases and were ranked as I-3, IIIb-1 and IVb-1 according to Clavien – Dindo classification.Conclusion. The degree of risk of severe post-resection hepatic failure should be considered one of the main criteria in choosing a strategy for radical surgical treatment. New surgical approaches (laparoscopic vascular isolation of the portal blood flow, using temporary hemostasis in laparoscopic hepatic resections and the device for its implementation) in resection surgery of primary hepatic cancer can improve the immediate results of treatment

Poloidal inhomogeneity of the particle fluctuation induced fluxes near of the LCFS at lower hybrid heating and improved confinement transition at the FT-2 tokamak

This paper deals with the new spectral and microturbulence experimental data and their analysis, which show, that the radial electric field Er generated at the LH heating (LHH) in the FT-2 is high enough to form the transport barriers. The ETB is formed when LHH is switched off. The radial fluctuation-induced EB drift flux densities near LCFS in SOL are measured at two different poloidal angles. For this purpose two Langmuir probes located at low and high field sides of the torus are used. Registration of the poloidal and radial components of the electric field and density fluctuations at the same time during one discharge permits to measure the poloidal asymmetry of the transport reduction mechanism of the radial and poloidal particle fluxes in the SOL. The absolute E(~) fluctuation levels show dependence on the sign of Er shear. The modification of the microscale turbulence by the poloidal Er x B rotation shear EB at the L - H transition near LCFS is also studied by X-mode fluctuation Reflectometry. The new data were obtained by spatial spectroscopic technique.Comment: 12th International Congress on Plasma Physics, 25-29 October 2004, Nice (France

ACTIVATIONAL SYSTEM BASED ON POLYMER COMPOSITIONAL MATERIAL FOR USE IN SMALL SPACECRAFTS, NANO- AND PICOSATELLITES

Работа связана с поиском, анализом научно-технической информации о внешних условиях в открытом космосе и работоспособности конструкции в заданных условия; созданием модели распространения тепла в системе; проектированием – расчетами, подтверждающими работоспособность конструкции и эскизным проектированием конструкции.The work is connected with search and analysis of scientific and technical documentation on environmental conditions in outer space and structure working capacities in those conditions; creation of heat transmission model in the system; designing – calculations to confirm working capacities of the structure and schematic design of the structure

MODERNIZATION OF ACTIVATED SYSTEM ON THE BASIS PKM WITH EFFECT OF SHAPE MEMORY FOR DISCLOSURE OF SOLAR PANELS IN SMALL SPACECRAFTS

Work is connected with search, the analysis of scientific and technical information on external conditions in an outer space and operability of a design in set conditions; analysis of research works on this subject; development of a way of modernization of activated system on the basis of PKM with effect of shape memory.Работа связана с поиском, анализом научно-технической информации о внешних условиях в открытом космосе и работоспособности конструкции в заданных условия; анализом научно-исследовательских работ по данной тематике; разработкой способа модернизации активаторной системы на основе полимерного композиционного материала (ПКМ) с эффектом памяти формы

БИОЛОГИЧЕСКИЕ РЕСУРСЫ ОЗЕРА ГУСИНОЕ СЕЛЕНГИНСКОГО РАЙОНА РЕСПУБЛИКИ БУРЯТИЯ

The research is conducted on Gusinoe lake in Selenginskiy district of theRepublicofByryatiya. The lake is one of the biggest in the Gusino-Ubukinskaya system and it suffers from anthropogenic impact. The wastewaters of Hydro Power Plant and wastewaters of the settlement flow into the lake where the water is used for drinking. The authors explored thelakebioresourcesand registered 4 main cropping. The main part of biomass contains perch – Perca fluviatilis (L.), roach – Rutillus rutilus L.) and pike – Esoxlucius (L.). The authors explored the dimensionally species variation based on the analysis of research skinning and application of heterobrochate nets. The research explores the data on the age distribution in the population. The authors observed perch, roach and pike in the fish fauna of Gusinoe lake where perch dominated 93.1% on the number and 77.1% on biomass. The roach can be referred to the species of common occurrence when its share in controlling yields was 6.4% on the number and 16.4% on the mass. The proportion of pike in the biomass was 6.3%. The perch population is characterized by dominating species aged 2+ to 4+. The researchers observed insufficient number of the fish aged 6+ to 11+ in the population. The roach is characterized by the fish aged 1+ to 12+ where the species aged 3+, 8+ and 10+ dominate. The linear growth of perch is steady during 20 years. The perch growth indicator was 11% higher than average in Gusinoe lake that that in the basins of Baikal region.Исследования проведены на оз. Гусиное, расположенном вСеленгинском районе Республики Бурятия. Озеро является одним из крупнейших в Гусино-Убукунской системе озер и в настоящее время подвергается сильнейшему антропогенному воздействию. В озеро поступают сточные воды ГРЭС и непосредственно стоки самого населенного пункта, воду используют для питьевых целей. Нами были проведены исследования по изучению биоресурсов озера. В ихтиофауне зарегистрированы четыре основных промысловых вида. Основу численности биомассы составляли окунь – Perca fluviatilis (L.), плотва – Rutillus rutilus (L.), щука – Esoxlucius (L.). Нами была изучена размерно-видовая изменчивость на основе анализов научных съемок с использованием разноячеистых сетей. Были получены также данные по возрастному распределению в популяции. В период 2013–2016 гг. в ихтиофауне оз. Гусиное отмечены окунь, плотва, щука. Доминировал в уловах окунь – 93,1% по численности и 77,1% по массе. Плотву сибирскую можно отнести к обычно встречающимся видам, ее доля в контрольных уловах по численности составила 6,4, по массе – 16,4%. Удельный вес щуки по биомассе – 6,3%. У окуня в популяции преобладают особи от 2+ до 4+. Рыбы в возрасте от 6+ до 11+ составляют незначительное количество популяции. Плотва представлена рыбами в возрасте от 1+ до 12+, преобладают особи 3+, 8+ и 10+. Линейный рост окуня остается стабильным в течение 20 лет. Показатель роста окуня в оз. Гусиное был на 11% выше средних для водоемов Забайкалья значений и находился на уровне предыдущих лет

Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility

The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or growth at elevated temperatures) can induce mutations in the host chromosomal DNA, we developed a specific and reliable method to eliminate pXO1 from B. anthracis using plasmid incompatibility. Three putative replication origins of pXO1 were inserted into a temperature-sensitive plasmid to generate three incompatible plasmids. One of the three plasmids successfully eliminated the large plasmid pXO1 from B. anthracis vaccine strain A16R and wild type strain A16. These findings provided additional information about the replication/partitioning of pXO1 and demonstrated that introducing a small incompatible plasmid can generate plasmid-cured strains of B. anthracis without inducing spontaneous mutations in the host chromosome

Высокие ампутации нижних конечностей

Objective:  to develop technology for high amputations of the lower extremities.Materials and methods. We analyzed the materials of the Nizhny Novgorod branch of the Federal State Unitary Enterprise “Moscow Orthopedic and Orthopedic Enterprise” Ministry of Labor of Russia for the period 2004–2018, the operation logs of the purulent surgery department of PIMU (1971–2018), and the electronic medical history “Program 1C” (2006–2012) and “Medialogue” (2013–2018) in all departments of PIMU, where high amputations of the lower extremities are possible performed.Results. Between 2006 and 2018, 391 lower limbs were cut off in 289 patients (above the knee – 128, below the knee – 263). With diabetic foot syndrome, 135 (34.5 %) amputations were performed, due to burns and frostbite, 73 (18.7 %) operations were made, while arterial atherosclerosis – 57 (14.6 %) operations, and the rest – about the consequences of injuries, tumors, malformations and vicious stumps. The number of amputations on the shin is 2 times more than on the hip. A technology has been developed for the formation of a new support and movement organ, including amputation of the lower leg, anesthesia, intensive care, and rehabilitation treatment. Conclusion. An analysis of the initial applications for prosthetics showed that the most frequent high amputations of the lower extremities are performed due to gangrene in arterial atherosclerosis (53.9 %) and diabetes mellitus (24.9 %). High amputations of the lower extremities at the hip level are performed in 60.4 % of patients and only in 30.1 % of patients – at the shin level. Malformations and diseases of the stumps of the thigh and lower leg are detected in 15.4 % of patients. The gold standard should be recognized as myoplastic amputation at the border of the upper and middle third of the shin with excision of the soleus muscle. Preservation of the knee joint creates the conditions for optimal prosthetics and timely social adaptation of patients. Цель исследования: разработка технологии выполнения высоких ампутаций нижних конечностей. Материалы и методы исследования. Анализировали материалы филиала «Нижегородский» ФГУП «Московское ПрОП» Минтруда России за 2004–2018 годы, операционные журналы отделения гнойной хирургии ПИМУ за 1971–2018 годы, электронные истории болезней «Программа 1С» за 2006–2012 годы и «Медиалог» за 2013–2018 годы по всем отделениям ПИМУ, где возможно выполнение высоких ампутаций нижних конечностей. Результаты исследования. За период с 2006 по 2018 год у 289 пациентов было проведено 391 отсечение нижних конечностей (бедро – 128, голень – 263). При синдроме диабетической стопы провели 135 (34,5 %) ампутаций, вследствие ожогов и отморожений – 73 (18,7 %) операции, при атеросклерозе артерий – 57 (14,6 %) вмешательств, а остальные выполнили по поводу последствий травм, опухолей, пороков развития и порочных культей. Число ампутаций на уровне голени в 2 раза выше, чем на уровне бедра. Разработана технология формирования нового органа опоры и перемещения, включающая ампутацию голени, обезболивание, интенсивную терапию, реабилитационное лечение. Заключение. Анализ первичных обращений на протезирование показал, что наиболее часто высокие ампутации нижних конечностей выполняют вследствие гангрены при атеросклерозе артерий (53,9 %) и сахарном диабете (24,9 %). Высокие ампутации нижних конечностей на уровне бедра проводят у 60,4 % больных и лишь у 30,1 % пациентов – на уровне голени. Пороки и болезни культей бедра и голени выявляют у 15,4 % пациентов. Золотым стандартом следует признать миопластическую ампутацию на границе верхней и средней третей голени с иссечением камбаловидной мышцы. Сохранение коленного сустава создает условия для оптимального протезирования и своевременной социально-бытовой адаптации пациентов.

Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola

© 2017, The Author(s). Hydrogen sulfide (H2S) is known to be an important signalling molecule in both animals and plants, despite its toxic nature. In plants it has been seen to control stomatal apertures, so altering the ability of bacteria to invade plant tissues. Bacteria are known to generate H2S as well as being exposed to plant-generated H2S. During their interaction with plants pathogenic bacteria are known to undergo alterations to their genomic complement. For example Pseudomonas syringae pv. phaseolicola (Pph) strain 1302A undergoes loss of a section of DNA known as a genomic island (PPHGI-1) when exposed to the plants resistance response. Loss of PPHGI-1 from Pph 1302A enables the pathogen to overcome the plants resistance response and cause disease. Here, with the use of H2S donor molecules, changes induced in Pph 1302A genome, as demonstrated by excision of PPHGI-1, were investigated. Pph 1302A cells were found to be resistant to low concentrations of H2S. However, at sub-lethal H2S concentrations an increase in the expression of the PPHGI-1 encoded integrase gene (xerC), which is responsible for island excision, and a subsequent increase in the presence of the circular form of PPHGI-1 were detected. This suggests that H2S is able to initiate excision of PPHGI-1 from the Pph genome. Therefore, H2S that may emanate from the plant has an effect on the genome structure of invading bacteria and their ability to cause disease in plants. Modulation of such plant signals may be a way to increase plant defence responses for crops in the future