97 research outputs found
Single-Photon Molecular Cooling
We propose a general method to cool the translational motion of molecules.
Our method is an extension of single photon atomic cooling which was
successfully implemented in our laboratory. Requiring a single event of
absorption followed by a spontaneous emission, this method circumvents the need
for a cycling transition and can be applied to any paramagnetic or polar
molecule. In our approach, trapped molecules would be captured near their
classical turning points in an optical dipole or RF-trap following an
irreversible transition process
Hanle effect in the CN violet system with LTE modeling
Weak entangled magnetic fields with mixed polarity occupy the main part of
the quiet Sun. The Zeeman effect diagnostics fails to measure such fields
because of cancellation in circular polarization. However, the Hanle effect
diagnostics, accessible through the second solar spectrum, provides us with a
very sensitive tool for studying the distribution of weak magnetic fields on
the Sun. Molecular lines are very strong and even dominate in some regions of
the second solar spectrum. The CN system is
one of the richest and most promising systems for molecular diagnostics and
well suited for the application of the differential Hanle effect method. The
aim is to interpret observations of the CN
system using the Hanle effect and to obtain an estimation of the magnetic field
strength. We assume that the CN molecular layer is situated above the region
where the continuum radiation is formed and employ the single-scattering
approximation. Together with the Hanle effect theory this provides us with a
model that can diagnose turbulent magnetic fields. We have succeeded in fitting
modeled CN lines in several regions of the second solar spectrum to
observations and obtained a magnetic field strength in the range from 10--30 G
in the upper solar photosphere depending on the considered lines.Comment: Accepted for publication in Astronomy and Astrophysic
Modelling the molecular Zeeman effect in M-dwarfs: methods and first results
We present first quantitative results of the surface magnetic field
measurements in selected M-dwarfs based on detailed spectra synthesis conducted
simultaneously in atomic and molecular lines of the FeH Wing-Ford
transitions. A modified version of the Molecular
Zeeman Library (MZL) was used to compute Land\'e g-factors for FeH lines in
different Hund's cases. Magnetic spectra synthesis was performed with the
Synmast code. We show that the implementation of different Hund's case for FeH
states depending on their quantum numbers allows us to achieve a good fit to
the majority of lines in a sunspot spectrum in an automatic regime. Strong
magnetic fields are confirmed via the modelling of atomic and FeH lines for
three M-dwarfs YZ~CMi, EV~Lac, and AD~Leo, but their mean intensities are found
to be systematically lower than previously reported. A much weaker field
(~kG against ~kG) is required to fit FeH lines in the spectra of
GJ~1224. Our method allows us to measure average magnetic fields in very
low-mass stars from polarized radiative transfer. The obtained results indicate
that the fields reported in earlier works were probably overestimated by about
\%. Higher quality observations are needed for more definite results.Comment: Accepted by A&A, 13 pages, 7 figures, 1 tabl
A Conduit System Distributes Chemokines and Small Blood-borne Molecules through the Splenic White Pulp
Access to the splenic white pulp is restricted to lymphocytes and dendritic cells. Here we show that movement of molecules from the blood into these confined areas is also limited. Large molecules, such as bovine serum albumin (68 kD), immunoglobulin G (150 kD), and 500 kD dextran are unable to enter the white pulp, whereas smaller blood-borne molecules can directly permeate this compartment. The distribution is restricted to a stromal network that we refer to as the splenic conduit system. The small lumen of the conduit contains collagen fibers and is surrounded in the T cell areas by reticular fibroblasts that express ER-TR7. It also contains the chemokine CCL21. Conversely, in B cell follicles the B cell–attracting chemokine CXCL13 was found to be associated with the conduit and absence of ER-TR7+ fibroblasts. These results show heterogeneity of reticular fibroblasts that enfold the conduit system and suggest that locally produced chemokines are transported through and presented on this reticular network. Therefore, the conduit plays a role in distribution of both blood-borne and locally produced molecules and provides a framework for directing lymphocyte migration and organization of the splenic white pulp
Radiative association and inverse predissociation of oxygen atoms
The formation of \mbox{O}_2 by radiative association and by inverse
predissociation of ground state oxygen atoms is studied using
quantum-mechanical methods. Cross sections, emission spectra, and rate
coefficients are presented and compared with prior experimental and theoretical
results. At temperatures below 1000~K radiative association occurs by approach
along the state of \mbox{O}_2 and above 1000~K inverse
predissociation through the \mbox{B}\,{}^3\Sigma_u^- state is the dominant
mechanism. This conclusion is supported by a quantitative comparison between
the calculations and data obtained from hot oxygen plasma spectroscopy.Comment: submitted to Phys. Rev. A (Sept. 7., 1994), 19 pages, 4 figures,
latex (revtex3.0 and epsf.sty
The circumstellar shell of the post-AGB star HD 56126: the CN/CN isotope ratio and fractionation
We have detected circumstellar absorption lines of the CN and
CN Violet and Red System in the spectrum of the post-AGB star HD~56126.
From a synthetic spectrum analysis, we derive a Doppler broadening parameter of
km~s, CN/CN, and a lower limit of
on CN/CN and CN/CN. A simple
chemical model has been computed of the circumstellar shell surrounding
HD~56126 that takes into account the gas-phase ion-molecule reaction between CN
and C. From this we infer that this reaction leads to isotopic
fractionation of CN. Taking into account the isotopic exchange reaction and the
observed CN/CN we find C/C (for K). Our analysis suggests that CN has a somewhat higher
rotational temperature than CN: and
K respectively. We identify possible causes for this difference in excitation
temperature, among which the dependence of the isotopic exchange
reaction.Comment: 24 pages, 6 figues Apj accepte
Controlled production of atomic oxygen and nitrogen in a pulsed radio-frequency atmospheric-pressure plasma
International audienceRadio-frequency driven atmospheric pressure plasmas are efficient sources for the production of reactive species at ambient pressure and close to room temperature. Pulsing the radio-frequency power input provides additional control over species production and gas temperature. Here, we demonstrate the controlled production of highly reactive atomic oxygen and nitrogen in a pulsed radio-frequency ( ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn001.gif] 13.56 MHz) atmospheric-pressure plasma, operated with a small ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn002.gif] 0.1 % air-like admixture ( ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn003.gif] \rm N_2 / ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn004.gif] \rm O_2 at ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn005.gif] 4:1 ) through variations in the duty cycle. Absolute densities of atomic oxygen and nitrogen are determined through vacuum-ultraviolet absorption spectroscopy using the DESIRS beamline at the SOLEIL synchrotron coupled with a high resolution Fourier-transform spectrometer. The neutral-gas temperature is measured using nitrogen molecular optical emission spectroscopy. For a fixed applied-voltage amplitude (234?V), varying the pulse duty cycle from 10% to 100% at a fixed 10?kHz pulse frequency enables us to regulate the densities of atomic oxygen and nitrogen over the ranges of ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn006.gif] (0.18±0.03) ? ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn007.gif] (3.7±0.1)× 10^20 ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn008.gif] \rm m^-3 and ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn009.gif] (0.2±0.06) ? ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn010.gif] (4.4±0.8) × 10^19 ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn011.gif] \rm m^-3 , respectively. The corresponding 11?K increase in the neutral-gas temperature with increased duty cycle, up to a maximum of ##IMG## [http://ej.iop.org/images/0022-3727/50/45/455204/daa8da2ieqn012.gif] (314±4) K, is relatively small. This additional degree of control, achieved through regulation of the pulse duty cycle and time-averaged power, could be of particular interest for prospective biomedical applications
Porcine Sialoadhesin (CD169/Siglec-1) Is an Endocytic Receptor that Allows Targeted Delivery of Toxins and Antigens to Macrophages
Sialoadhesin is exclusively expressed on specific subpopulations of macrophages. Since sialoadhesin-positive macrophages are involved in inflammatory autoimmune diseases, such as multiple sclerosis, and potentially in the generation of immune responses, targeted delivery of drugs, toxins or antigens via sialoadhesin-specific immunoconjugates may prove a useful therapeutic strategy. Originally, sialoadhesin was characterized as a lymphocyte adhesion molecule, though recently its involvement in internalization of sialic acid carrying pathogens was shown, suggesting that sialoadhesin is an endocytic receptor. In this report, we show that porcine sialoadhesin-specific antibodies and F(ab')2 fragments trigger sialoadhesin internalization, both in primary porcine macrophages and in cells expressing recombinant porcine sialoadhesin. Using chemical inhibitors, double immunofluorescence stainings and dominant-negative constructs, porcine sialoadhesin internalization was shown to be clathrin- and Eps15-dependent and to result in targeting to early endosomes but not lysosomes. Besides characterizing the sialoadhesin endocytosis mechanism, two sialoadhesin-specific immunoconjugates were evaluated. We observed that porcine sialoadhesin-specific immunotoxins efficiently kill sialoadhesin-expressing macrophages. Furthermore, porcine sialoadhesin-specific albumin immunoconjugates were shown to be internalized in macrophages and immunization with these immunoconjugates resulted in a rapid and robust induction of albumin-specific antibodies, this compared to immunization with albumin alone. Together, these data expand sialoadhesin functionality and show that it can function as an endocytic receptor, a feature that cannot only be misused by sialic acid carrying pathogens, but that may also be used for specific targeting of toxins or antigens to sialoadhesin-expressing macrophages
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