744 research outputs found

    Paternal mtDNA and Maleness Are Co-Inherited but Not Causally Linked in Mytilid Mussels

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    BACKGROUND: In marine mussels of the genus Mytilus there are two mitochondrial genomes. One is transmitted through the female parent, which is the normal transmission route in animals, and the other is transmitted through the male parent which is an unusual phenomenon. In males the germ cell line is dominated by the paternal mitochondrial genome and the somatic cell line by the maternal. Research to date has not allowed a clear answer to the question of whether inheritance of the paternal genome is causally related to maleness. METHODOLOGY/PRINCIPAL FINDINGS: Here we present results from hybrid crosses, from triploid mussels and from observations of sperm mitochondria in fertilized eggs which clearly show that maleness and presence of the paternal mitochondrial genome can be decoupled. These same results show that the female mussel has exclusive control of whether her progeny will inherit the mitochondrial genome of the male parent. CONCLUSIONS/SIGNIFICANCE: These findings are important in our efforts to understand the mechanistic basis of this unusual mode of mitochondrial DNA inheritance that is common among bivalves

    Geoscience at the nanometre scale: review of analytical transmission electron microscopy applications

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    This paper describes applications of analytical Transmission Electron Microscopy (TEM) in the geosciences. The topics include: 1) sulphide-mineral oxidation; 2) trace-metal attenuation by secondary Mn oxides; 3) silicate weathering; 4) transition-metal valence in minerals; and 5) secondary Hg minerals in stream sediments. The main advantage of the analytical TEM is the ability to obtain images, chemical information, and electron diffraction patterns at the nanometre scale. With such high spatial resolution, it is possible to observe physical and chemical features in samples that cannot be resolved with most other techniques. This information can lead to significant improvement in our understanding of the system under investigation. Sample preparation techniques that are used in each study are also described in this paper. The preparation of samples for TEM analysis can be challenging because of the heterogeneity commonly encountered in geological materials, the fragility of some geological samples (e.g., low-temperature minerals), and the need to maintain spatial relationships present in the samples. The sample preparation techniques presented are specific to the needs of the study and the appropriateness of these methods is demonstrated by the high quality analytical TEM data that are obtained. RÉSUMÉ Cet exposé décrit des applications de la microscopie électronique à transmission analytique dans les sciences de la terre. Les aspects étudiés comprennent : 1) l'oxydation des minéraux sulfurés; 2) l'atténuation des métaux-traces par des oxydes de Mn secondaires; 3) la silicatisation météorique; 4) la valence des métaux de transition dans les minéraux; et 5) les minéraux de Hg secondaires dans les sédiments fluviatiles. Le principal avantage qu'offre la MET analytique est la possibilité d'obtenir des images, des données chimiques et des figures de diffraction des électrons à l'échelle nanométrique. Une résolution spatiale aussi élevée permet l'observation dans les échantillons de propriétés physiques et chimiques impossibles à éclaircir au moyen de la majorité des autres techniques. De tels renseignements peuvent mener à une amélioration marquée de notre compréhension du système à l'étude. Cet exposé décrit en plus les techniques de préparation des échantillons utilisées lors de chaque étude. La préparation des échantillons à une analyse MET peut s'avérer compliquée en raison de l'hétérogénéité que présentent communément les matières géologiques, de la fragilité de certains échantillons géologiques (p. ex. minéraux à basse température) et de la nécessité de maintenir les liens spatiaux présents dans les échantillons. Les techniques de préparation des échantillons présentées sont propres aux besoins de l'étude; les données de haute qualité obtenues des analyses MET témoignent de la pertinence de ces méthodes. [Traduit par la rédaction

    Suppression of Phase Separation in LiFePO4 Nanoparticles During Battery Discharge

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    Using a novel electrochemical phase-field model, we question the common belief that LixFePO4 nanoparticles separate into Li-rich and Li-poor phases during battery discharge. For small currents, spinodal decomposition or nucleation leads to moving phase boundaries. Above a critical current density (in the Tafel regime), the spinodal disappears, and particles fill homogeneously, which may explain the superior rate capability and long cycle life of nano-LiFePO4 cathodes.Comment: 27 pages, 8 figure

    Simulations of extensional flow in microrheometric devices

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    We present a detailed numerical study of the flow of a Newtonian fluid through microrheometric devices featuring a sudden contraction–expansion. This flow configuration is typically used to generate extensional deformations and high strain rates. The excess pressure drop resulting from the converging and diverging flow is an important dynamic measure to quantify if the device is intended to be used as a microfluidic extensional rheometer. To explore this idea, we examine the effect of the contraction length, aspect ratio and Reynolds number on the flow kinematics and resulting pressure field. Analysis of the computed velocity and pressure fields show that, for typical experimental conditions used in microfluidic devices, the steady flow is highly three-dimensional with open spiraling vortical structures in the stagnant corner regions. The numerical simulations of the local kinematics and global pressure drop are in good agreement with experimental results. The device aspect ratio is shown to have a strong impact on the flow and consequently on the excess pressure drop, which is quantified in terms of the dimensionless Couette and Bagley correction factors. We suggest an approach for calculating the Bagley correction which may be especially appropriate for planar microchannels

    Report of the Scientific Council Meeting 01 -15 June 2017

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    Council met at the Sobey Building, Saint Mary’s University, Halifax, NS, Canada, during 01 – 15 June 2017, to consider the various matters in its Agenda. Representatives attended from Canada, Denmark (in respect of Faroe Islands and Greenland), the European Union (France, Germany (via WebEx), Portugal, Spain, the United Kingdom and the European Commission), Japan, the Russian Federation and the United States of America. Observers from the Ecology Action Centre and Dalhousie University were also present. The Executive Secretary, Scientific Council Coordinator and other members of the Secretariat were in attendance. The Executive Committee met prior to the opening session of the Council to discuss the provisional agenda and plan of work. The Council was called to order at 1000 hours on 01 June 2017. The provisional agenda was adopted with modification. The Scientific Council Coordinator was appointed the rapporteur. The Council was informed that the meeting was quorate and authorization had been received by the Executive Secretary for proxy votes from the European Union, Denmark (in respect of Faroe Islands and Greenland), Iceland, Japan, Republic of Korea, and Norway. The opening session was adjourned at 1200 hours on 01 June 2017. Several sessions were held throughout the course of the meeting to deal with specific items on the agenda. The Council considered adopted the STACFEN report on 8 June 2017, and the STACPUB, STACFIS and STACREC reports on 15 June 2017. The concluding session was called to order at 0830 hours on 15 June 2017. The Council considered and adopted the report the Scientific Council Report of this meeting of 01 -15 June 2017. The Chair received approval to leave the report in draft form for about two weeks to allow for minor editing and proof-reading on the usual strict understanding there would be no substantive changes. The meeting was adjourned at 1430 hours on 15 June 2017. The Reports of the Standing Committees as adopted by the Council are appended as follows: Appendix I - Report of the Standing Committee on Fisheries Environment (STACFEN), Appendix II - Report of Standing Committee on Publications (STACPUB), Appendix III - Report of Standing Committee on Research Coordination (STACREC), and Appendix IV - Report of Standing Committee on Fisheries Science (STACFIS). The Agenda, List of Research (SCR) and Summary (SCS) Documents, and List of Representatives, Advisers and Experts, are given in Appendix V-VII. The Council’s considerations on the Standing Committee Reports, and other matters addressed by the Council follow in Sections II-XV
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