Histological exploration of follicular population of the Moroccan bovine (Oulmes-Zaers) breed

Follicular population and repartition in the ovarian cortex was investigated in the ovaries of the Oulmès-Zaers cattle breed and its crosses. A total of 30 ovaries were collected at slaughterhouse in Casablanca and Rabat from Oulmes-Zaers breed and its crosses (2 genotypes) of 3 age groups (5 years). The histological study of the ovary revealed that the ovarian cortex is composed of different areas (from the periphery to the medulla), the non stratified epithelium of the ovary, the tunica albuginea composed of two areas rich of collagen fibers directed into different ways white variable thickness. The primordial and antral follicles appear in underlying areas. Therefore, the follicles cannot appear on the ovarian surface when situated under thicker Layers. At the quantitative level, the ovary of the Oulmes-Zaears breed seems to contain less follicles in animals less than 3 years of age as compared to the crossbreed (P< 0.05). Between 3 and 5 years of age, the follicles occupied similar areas of the ovaries (17% and 18% for oulmes-Zaers and its crosses, respectively). This study shows that the ovary of the Moroccan local cattle breed (Oulmes-Zaers) is equipped with a whole plain follicular population that is more important than the one visible on the surface of the ovary and which is exploited by aspiration and picking techniques. Key words: Ovary, Oulmès-Zaers, follicular population, bovine, age. African Journal of Biotechnology Vol.3(5) 2004: 294-29

Genetic and nongenetic effects on the number of ovarian follicles and oocyte yield and quality in the bovine local (Oulmes Zaer), exotic breeds and their crosses in Morocco

The effects of genetic and non genetic factors on the number of ovarian follicles and oocyte yield and quality in the bovine local breed (Oulmes Zaer), exotic breeds and their crosses in Morocco was investigated. In this study, females in very bad body conditions (BCS < 2) were not slaughtered and the average. The body condition scores (BCS) was 2.94 ± 0.89. Although some individual values were out of the normal ranges, mean values of total proteins, albumin, urea, &#946;-OH and GOT remain normal and were 77.83 ± 8.74 g/l, 32.4 ± 4.41g/l, 4.43 ± 2.13 mmol/l , 0.83± 0.48 mmol/l et 45.55 ± 11.95 UI/l, respectively. The mean number of ovarian follicles per cow (2-8 mm) was high (22.98 ± 8.41) whereas the oocyte yield (and 2.60 ± 1.53) was very low. The effects of genetic group, age and BCS on the number of follicles, oocyte yield and the quality were significant. Key Words: Cows, follicular population, oocyte yield and quality. African Journal of Biotechnology Vol.4(1) 2005: 9-1

Taxonomic diversity of benthic macroinvertebrates along the Oum Er Rbia River (Morocco): implications for water quality bio-monitoring using indicator species

The macroinvertebrates of the Oum Er Rbia River were studied from samples collected seasonally from September 2015 to September 2016 at 10 sampling sites. The macroinvertebrates found during the sampling period were distributed into twelve orders. The most abundant order was diptera, having 9618 individuals, followed by the order Ephemeroptera with 2985 individuals. Coleoptera, odonates and crustaceans represent only a small fraction of the total fauna. Hydropsyche, Chironomidae sp. and Simuliidae are numerically more inventoried. The composition and distribution of the species were directly or indirectly affected by the physicochemical variables and the quality of the habitat. Correspondence analysis results showed that habitat quality and quality of water represented species distribution patterns and species can be used as indicators to assess the quality of the Oum Er Rbia River system. Habitat management along the Oum Er Rbia river should be aimed at preserving native species, especially during the summer, when the biotope requirements are optimal. The results obtained in this study showed an alarming situation of the water quality of the Oum Er Rbia River and particularly in downstream segment. To solve this problem, we recommend the development of the wastewater discharge of Khenifra and Kasba Tadla and the purification of wastewater before it is discharged into the river

Mise en place d’un projet de préparation des Kits de radiologie au sein de la pharmacie de l’Institut National d’Oncologie.

Introduction : La dispensation nominative des produits de santé est une pratique recommandée sur l’échelle nationale et  internationale .L’objectif de ce travail est de décrire le projet  de mise en place d’une unité de préparation et de dispensation des kits de radiologie au sein de la pharmacie de l’institut national d’oncologie.Matériel et méthode : Nous avons préparé en concertation avec le service de radiologie trois types de kits, deux pour le scanner contenant comme produit de contraste l’Ultravist® 50 ml ou 100 ml et le troisième pour l’imagerie par résonance magnétique (IRM)  contenant  le Gadovist®. Les kits contiennent  aussi les dispositifs médicaux nécessaires pour l’administration des produits de contraste. La traçabilité et le suivi de la consommation ont été assurés tout au long de la mise en place du projet.Résultats : Durant le premier mois suivant la mise en place de ce système de dispensation, nous avons préparé 288 kits IRM, 184 kits scanner 100 ml et 60 kits scanner 50ml. La traçabilité des kits IRM montre une consommation de 154, le  sexe ratio des patients est de 1.53, 69% des patients étaient ramedistes, 64% sont des patients de l’institut national d’oncologie.Conclusion : La mise en place du projet de préparation des kits  de radiologie permet  une  standardisation de la pratique, une meilleure traçabilité des produits pharmaceutiques et un gain économique pour l’hôpital, d’où l’intérêt de maintenir ce projet et le développer

Potential of Carbon Absorption Mangrove Forest at Sarawet Village Kuala Batu, East Likupang, North Minahasa Regency

Mangrove forest is one of a coastal natural resource with abundant potentials. The rapid coastal development has cost bad effects, such as mangrove forest conversion into dike or tourism. Mangrove forest has a prominent ecological function for coastal area. The purpose of this study was to analyst carbon absorption potency in both natural and restored mangrove forest in Sarawet Village, Kuala Batu, East Likupang. The sampling method in this study was a survey method that is observation and field sampling. The collected data was surface mangrove biomass and sediment, then analyst in Sam Ratulangi Laboratory, Manado. The biomass sampling data using transect line quadrat while and sediment sampling using sediment corer. This study found. That conclude that natural mangrove forest have a higher absorption and restored potential than restored mangrove forest.Keywords :  mangrove, biomass, carbon, sediment AbstrakPotensi sumber daya hutan Indonesia sangat melimpah, dan salah satunya ialah hutan mangrove. Pembangunan pada daerah pesisir yang begitu cepat telah memberi dampak buruk terhadap lingkungan, seperti konversi lahan hutan mangrove menjadi tambak dan kawasan parawisata. Hutan mangrove merupakan salah satu hutan yang memiliki fungsi ekologis sangat penting terutama bagi wilayah pesisir. Tujuan penelitian ini adalah untuk menganalisis potensi penyerapan karbon pada hutan mangrove yang restorasi dan alami di Desa Sarawet Kuala Batu Likupang Timur. Metode pengambilan data yang digunakan dalam kegiatan penelitian ini ialah metode survey yakni pengamatan dan pengambilan sampel langsung dilapangan. Data yang diambil ialah data biomassa mangrove bagian atas dan sedimen. Sampel yang diambil di analisis di Laboratorium Terpadu Universitas Sam Ratulangi Manado. Untuk pengambilan data biomassa dilakukan dengan menggunakan garis transek kuadrat dan pengambilan sampel sedimen menggunakan sediment corer. Dari hasil penelitian yang diperoleh, menunjukan bahwa hutan mangrove yang alami memiliki potensi penyerapan dan simpanan karbon yang lebih tinggi dibandingkan dengan hutan mangrove yang direstorasi.   Kata kunci :  mangrove, biomassa, karbon, sedime

Risk factors for MERS coronavirus infection in dromedary camels in Burkina Faso, Ethiopia, and Morocco, 2015

Understanding Middle East respiratory syndrome coronavirus (MERS-CoV) transmission in dromedary camels is important, as they consitute a source of zoonotic infection to humans. To identify risk factors for MERS-CoV infection in camels bred in diverse conditions in Burkina Faso, Ethiopia and Morocco, blood samples and nasal swabs were sampled in February-March 2015. A relatively high MERS-CoV RNA rate was detected in Ethiopia (up to 15.7%; 95% confidence interval (CI): 8.2-28.0), followed by Burkina Faso (up to 12.2%; 95% CI: 7-20.4) and Morocco (up to 7.6%; 95% CI: 1.9-26.1). The RNA detection rate was higher in camels bred for milk or meat than in camels for transport (p = 0.01) as well as in younger camels (p = 0.06). High seropositivity rates (up to 100%; 95% CI: 100-100 and 99.4%; 95% CI: 95.4-99.9) were found in Morocco and Ethiopia, followed by Burkina Faso (up to 84.6%; 95% CI: 77.2-89.9). Seropositivity rates were higher in large/medium herds (≥51 camels) than small herds (p = 0.061), in camels raised for meat or milk than for transport (p = 0.01), and in nomadic or sedentary herds than in herds with a mix of these lifestyles (p < 0.005).published_or_final_versio

MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity

International audienceMiddle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa as well as in the Arabian Peninsula, zoonotic disease appears confined to the Arabian Peninsula. MERS-CoVs from Africa have hitherto been poorly studied. We genetically and phenotypically characterized MERS-CoV from dromedaries sampled in Morocco, Burkina Faso, Nigeria, and Ethiopia. Viruses from Africa (clade C) are phylogenetically distinct from contemporary viruses from the Arabian Peninsula (clades A and B) but remain antigenically similar in microneutralization tests. Viruses from West (Nigeria, Burkina Faso) and North (Morocco) Africa form a subclade, C1, that shares clade-defining genetic signatures including deletions in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in ex vivo cultures of human bronchus and lung. BF785 replicated to lower titer in lungs of human DPP4-transduced mice. A reverse genetics-derived recombinant MERS-CoV (EMC) lacking ORF4b elicited higher type I and III IFN responses than the isogenic EMC virus in Calu-3 cells. However, ORF4b deletions may not be the major determinant of the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to zoonotic potential. There is an urgent need for studies of MERS-CoV at the animal-human interface