Intracellular S1P Generation Is Essential for S1P-Induced Motility of Human Lung Endothelial Cells: Role of Sphingosine Kinase 1 and S1P Lyase

Earlier we have shown that extracellular sphingosine-1-phosphate (S1P) induces migration of human pulmonary artery endothelial cells (HPAECs) through the activation of S1P(1) receptor, PKCε, and PLD2-PKCζ-Rac1 signaling cascade. As endothelial cells generate intracellular S1P, here we have investigated the role of sphingosine kinases (SphKs) and S1P lyase (S1PL), that regulate intracellular S1P accumulation, in HPAEC motility

Food habits of the farmer damselfish Stegastes nigricans inferred by stomach content, stable isotope, and fatty acid composition analyses

The territorial damselfish, Stegastes nigricans, maintains algal farms by excluding invading herbivores and weeding unpalatable algae from its territories. In Okinawa, Japan, S. nigricans farms are exclusively dominated by Polysiphonia sp., a highly digestible filamentous rhodophyte. This study was aimed at determining the diet of S. nigricans in Okinawa and its dependency on these almost-monoculture algal farms based on stomach content and chemical analyses. Stomach content analyses revealed that all available food items in the algal farms (i. e., algae, benthic animal inhabitants, trapped detritus) were contained in fish stomachs, but amorphous organic matter accounted for 68% of the contents. Therefore, carbon and nitrogen stable isotope ratios and fatty acid (FA) compositions were analyzed to trace items actually assimilated in their bodies. Stable isotope analyses showed that benthic animals were an important food source even for this farmer fish. Two essential fatty acids (EFAs), 20:4n6 and 20:5n3, which are produced only by rhodophytes among available food items, were rich in the muscle tissue of S. nigricans as well as in algal mats and detritus, suggesting that algal mats contribute EFAs to S. nigricans directly and indirectly through the food web. In conclusion, S. nigricans ingested algal mats, detritus, and benthic animals maintained within its farm. Algae and detritus were original sources of EFAs, and benthic animals, which were much more abundant in the farms than in outside territories, provided a nitrogen-rich dietary source for the fish

МАТЕМАТИЧНЕ МОДЕЛЮВАННЯ ВІБРОВОЗМУЩАЮЩІХ СИЛ У КОРОТКОЗАМКНЕНИМ АСИНХРОННИМ ДВИГУНОМ З ПОШКОДЖЕННЯМ ОБМОТКИ РОТОРА

The mathematical model for analysis of vibro-disturbing electromagnetic forces in short-circuit asynchronousmotors at presence of the rotor winding damages is formulated. It is shown that the comparative analysis ofthese forces spectrogram allows to expose the rotor winding damages with high authenticity.Сформулирована математическая модель для анализа вибровозмущающих электромагнитныхсил в короткозамкнутых асинхронных двигателях при наличии повреждений обмотки ротора.Показано, что сравнительный анализ спектрограмм этих сил позволяет с высокой достоверностью выявлять повреждения обмотки ротора.Сформульовано математичну модель для аналізу віброзбуджуючих електромагнітних сил в короткозамкнених асинхронних двигунах при наявності ушкоджень обмотки ротора. Показано, щопорівняльний аналіз спектрограм зазначених сил дозволяє з високою вірогідністю виявляти пошкодження обмотки ротора

МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ ВИБРОВОЗМУЩАЮЩИХ СИЛ В КОРОТКОЗАМКНУТОМ АСИНХРОННОМ ДВИГАТЕЛЕ С ПОВРЕЖДЕННОЙ ОБМОТКОЙ РОТОРА

Сформулирована математическая модель для анализа вибровозмущающих электромагнитных сил в короткозамкнутых асинхронных двигателях при наличии повреждений обмотки ротора. Показано, что сравнительный анализ спектрограмм этих сил позволяет с высокой достоверностью выявлять повреждения обмотки ротора

МАТЕМАТИЧНЕ МОДЕЛЮВАННЯ ВІБРОВОЗМУЩАЮЩІХ СИЛ У КОРОТКОЗАМКНЕНИМ АСИНХРОННИМ ДВИГУНОМ З ПОШКОДЖЕННЯМ ОБМОТКИ РОТОРА

The mathematical model for analysis of vibro-disturbing electromagnetic forces in short-circuit asynchronousmotors at presence of the rotor winding damages is formulated. It is shown that the comparative analysis ofthese forces spectrogram allows to expose the rotor winding damages with high authenticity.Сформулирована математическая модель для анализа вибровозмущающих электромагнитныхсил в короткозамкнутых асинхронных двигателях при наличии повреждений обмотки ротора.Показано, что сравнительный анализ спектрограмм этих сил позволяет с высокой достоверностью выявлять повреждения обмотки ротора.Сформульовано математичну модель для аналізу віброзбуджуючих електромагнітних сил в короткозамкнених асинхронних двигунах при наявності ушкоджень обмотки ротора. Показано, щопорівняльний аналіз спектрограм зазначених сил дозволяє з високою вірогідністю виявляти пошкодження обмотки ротора

Characterisation of the cell membrane associated products of the Neuregulin 4 gene.

The NRG4 gene is a member of a family of four genes which encode a class of epidermal growth factors. This gene has been reported to express a protein designated here as NRG4A1. We describe here a novel splice variant of the NRG4 gene, NRG4A2 which encodes a C-terminal region containing a predicted type I PDZ binding peptide. Both NRG4A1 and NRG4A2 were shown to be expressed on the cell surface, as expected by the presence of a predicted transmembrane sequence, and were modified at a single N-linked glycosylation site in the extracellular domain. Significant stabilisation of expression of both proteins was seen in the presence of the proteosome inhibitor MG-132 suggesting that they are normally degraded by this system. N-terminal cleavage was inhibited in both isotypes by the broad-spectrum matrix metalloproteinase inhibitor, galardin (GM 6001). A glycosylated, secreted form of NRG4A1 was detected in the cell medium which showed biologically activity in two assays, phosphorylation of the HER4 receptor and stimulation of neurite formation in PC-12 cells stably expressing HER4. Transfection and expression of GFP-tagged proteins and immunofluorescent staining with specific anti-peptide antibodies showed that NRG4A1 is localised to membrane ruffles while NRG4A2 has a more punctate membrane distribution

TEMPERATURE FEATURES OF HYSTERESIS PROPERTIES OF La-Co FILMS

Magnetic properties of La-Co films of different compositions are investigated experimen-tally within the temperature range of 5-350 K and interpreted with involvement of computer modeling. Exchange bias is detected, its presence is associated with amorphous-crystalline inhomogeneity of the films.This work was supported by the Russian Science Foundation, project № 18-72-10044

An inducible arylsulfatase of Volvox carteri with properties suitable for a reporter-gene system. Purification, characterization and molecular cloning

The multicellular green flagellate Volvox carteri synthesizes a periplasmic arylsulfatase in response to sulfur deprivation. The inducible enzyme has been purified to homogeneity and characterized. The corresponding gene and cDNA have been cloned. Determination of the sequence of genomic clones and comparisons to the cDNA sequence, revealed sixteen introns and seventeen exons that encode a 649-amino-acid polypeptide chain. Since the arylsulfatase enzyme is readily assayed using chromogenic substrates, but is not detectable in cells grown in sulfate-containing medium, the gene encoding arylsulfatase may be useful as a reporter gene in V. carteri. In addition, the highly regulated promoter of the arylsulfatase gene suggests its suitability as a tool for producing inducible expression vectors for cloned genes