Assembléias de aranhas em manchas de cerrado amplamente separadas no Estado de São Paulo, Brasil

Spider Assemblages in widely-separated patches of cerrado in São Paulo State, BrazilAssembléias de aranhas em manchas de cerrado amplamente separadas no Estado de São Paulo, Brasil

Factorial and response surface designs robust to missing observations

Made available in DSpace on 2018-11-26T17:35:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-09-01Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Compound optimum design criteria which allow pure error degrees of freedom may produce designs that break down when even a single run is missing, if the number of experimental units is small. The inclusion, in the compound criteria, of a measure of leverage uniformity is proposed in order to produce designs that are more robust to missing observations. By appropriately choosing the weights of each part of the criterion, robust designs are obtained that are also highly efficient in terms of other properties. Applications to various experimental setups show the advantages of the new methods. (C) 2016 Elsevier B.V. All rights reserved.USP UFSCar, Programa Interinst Posgrad Estat, Sao Carlos, SP, BrazilKings Coll London, Dept Math, London, EnglandUniv Estadual Paulista, Dept Bioestat, IB, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Bioestat, IB, Botucatu, SP, BrazilFAPESP: 2014/01818-

Avaliação da maturação nuclear in vitro de oócitos de gatas domésticas (Felis catus) pré-púberes e púberes

O objetivo do trabalho foi avaliar a taxa de maturação nuclear in vitro de oócitos provenientes de gatas doméstica púbere e pré-púbere. Foram utilizadas 15 fêmeas felinas, 10 púberes e 5 pré-púberes; sendo os oócitos obtidos por aspiração quantificados e classificados. Os oócitos classificados como excelentes e regulares foram reunidos em grupos de 10, em meio de cultura, recobertos em óleo mineral em Placas de Petri siliconizadas e descartáveis. Após permanência em estufa, a 38°C e 5% de CO2 por 48 horas, os oócitos foram submetidos a duas lavagens com solução de hialuronidase a 0,4%, fixados em metanol/acido acético e corados com orceína acética. A avaliação da configuração cromossômica de oócitos maturados in vitro resultou em 44,68% das células em metáfase II no grupo das fêmeas púberes e 25,32% no grupo das doadoras pré-púberes, indicando que a puberdade influencia a capacidade dos oócitos se desenvolverem in vitro.This study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. Fifteen ovaries were used; 10 from adult females and 5 from young females. The oocytes collected by aspiration were quantified and classified. The oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable Petri dishes. The oocyte were incubated at 38°C and 5% de CO2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. Evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase II in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development

Portugal e os BRIC: numa perspectiva da diplomacia pública e da autopoiesis

O sistema internacional post-­‐11 de Setembro vem acelerar a movimentação em torno da sociedade civil, alterando, consequentemente, o próprio conceito espácio-­temporal da actuação da diplomacia, na sua acepção clássica, na arena internacional enquanto instrumento pacífico de execução da política externa. Assiste-se a uma complexidade crescente no processo de edificação da nova ordem mundial cujo epicentro se circunscreve numa espécie de sub-­‐mundialização à escala doméstica de cada Estado quer pela (in)capacidade da elite governante em responder aos estímulos provenientes do ambiente externo ao ritmo vertiginoso da velocidade dos fluxos de informação entre os mais diversos actores das relações internacionais. Daí que a prática da diplomacia tradicional tal como a conhecemos caminha para além da evolução teórica, situando-­se cada vez mais em termos concretos num mundo inconstante de caminho incerto com regimes antagónicos de convergências pontuais e imprevisíveis que levam à emergência de outras formas de actuação como sendo o caso da diplomacia pública que representa um recurso estratégico vital para os estados enquanto actores das relações internacionais e concretamente para o caso de Portugal nas suas relações com os BRIC onde se pretende melhorar e influenciar a imagem de um país quer internamente quer externamente como um país/marca num mundo competitivo

Beam commissioning of the 35 MeV section in an intensity modulated proton linear accelerator for proton therapy

This paper presents the experimental results on the Terapia Oncologica con Protoni-Intensity Modulated Proton Linear Accelerator (TOP-IMPLART) beam that is currently accelerated up to 35 MeV, with a final target of 150 MeV. The TOP-IMPLART project, funded by the Innovation Department of Regione Lazio (Italy), is led by Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) in collaboration with the Italian Institute of Health and the Oncological Hospital Regina Elena-IFO. The accelerator, under construction and test at ENEA-Frascati laboratories, employs a commercial 425 MHz, 7 MeV injector followed by a sequence of 3 GHz accelerating modules consisting of side coupled drift tube linac (SCDTL) structures up to 71 MeV and coupled cavity linac structures for higher energies. The section from 7 to 35 MeV, consisting on four SCDTL modules, is powered by a single 10 MW klystron and has been successfully commissioned. This result demonstrates the feasibility of a “fully linear” proton therapy accelerator operating at a high frequency and paves the way to a new class of machines in the field of cancer treatment

Genome-wide signatures of complex introgression and adaptive evolution in the big cats.

The great cats of the genus Panthera comprise a recent radiation whose evolutionary history is poorly understood. Their rapid diversification poses challenges to resolving their phylogeny while offering opportunities to investigate the historical dynamics of adaptive divergence. We report the sequence, de novo assembly, and annotation of the jaguar (Panthera onca) genome, a novel genome sequence for the leopard (Panthera pardus), and comparative analyses encompassing all living Panthera species. Demographic reconstructions indicated that all of these species have experienced variable episodes of population decline during the Pleistocene, ultimately leading to small effective sizes in present-day genomes. We observed pervasive genealogical discordance across Panthera genomes, caused by both incomplete lineage sorting and complex patterns of historical interspecific hybridization. We identified multiple signatures of species-specific positive selection, affecting genes involved in craniofacial and limb development, protein metabolism, hypoxia, reproduction, pigmentation, and sensory perception. There was remarkable concordance in pathways enriched in genomic segments implicated in interspecies introgression and in positive selection, suggesting that these processes were connected. We tested this hypothesis by developing exome capture probes targeting ~19,000 Panthera genes and applying them to 30 wild-caught jaguars. We found at least two genes (DOCK3 and COL4A5, both related to optic nerve development) bearing significant signatures of interspecies introgression and within-species positive selection. These findings indicate that post-speciation admixture has contributed genetic material that facilitated the adaptive evolution of big cat lineages

Cognitive behavioral therapy for insomnia in patients with mental disorders and comorbid insomnia: A systematic review and meta-analysis.

Almost 70% of patients with mental disorders report sleep difficulties and 30% fulfill the criteria for insomnia disorder. Cognitive behavioral therapy for insomnia (CBT-I) is the first-line treatment for insomnia according to current treatment guidelines. Despite this circumstance, insomnia is frequently treated only pharmacologically especially in patients with mental disorders. The aim of the present meta-analysis was to quantify the effects of CBT-I in patients with mental disorders and comorbid insomnia on two outcome parameters: the severity of insomnia and mental health. The databases PubMed, CINHAL (Ebsco) und PsycINFO (Ovid) were searched for randomized controlled trials on adult patients with comorbid insomnia and any mental disorder comparing CBT-I to placebo, waitlist or treatment as usual using self-rating questionnaires as outcomes for either insomnia or mental health or both. The search resulted in 1994 records after duplicate removal of which 22 fulfilled the inclusion criteria and were included for the meta-analysis. The comorbidities were depression (eight studies, 491 patients), post-traumatic stress disorder (PTSD, four studies, 216 patients), alcohol dependency (three studies, 79 patients), bipolar disorder (one study, 58 patients), psychosis (one study, 50 patients) and mixed comorbidities within one study (five studies, 189 patients). The effect sizes for the reduction of insomnia severity post treatment were 0.5 (confidence interval, CI, 0.3-0.8) for patients with depression, 1.5 (CI 1.0-1.9) for patients with PTSD, 1.4 (CI 0.9-1.9) for patients with alcohol dependency, 1.2 (CI 0.8-1.7) for patients with psychosis/bipolar disorder, and 0.8 (CI 0.1-1.6) for patients with mixed comorbidities. Effect sizes for the reduction of insomnia severity were moderate to large at follow-up. Regarding the effects on comorbid symptom severity, effect sizes directly after treatment were 0.5 (CI 0.1-0.8) for depression, 1.3 (CI 0.6-1.9) for PTSD, 0.9 (CI 0.3-1.4) for alcohol dependency in only one study, 0.3 (CI -0.1 - 0.7, insignificant) for psychosis/bipolar, and 0.8 (CI 0.1-1.5) for mixed comorbidities. There were no significant effects on comorbid symptoms at follow-up. Together, these significant, stable medium to large effects indicate that CBT-I is an effective treatment for patients with insomnia and a comorbid mental disorder, especially depression, PTSD and alcohol dependency. CBT-I is also an effective add-on treatment with the aim of improving mental health in patients with depression, PTSD, and symptom severity in outpatients with mixed diagnoses. Thus, in patients with mental disorders and comorbid insomnia, given the many side effects of medication, CBT-I should be considered as a first-line treatment