Self-synchronized Encryption for Physical Layer in 10Gbps Optical Links

In this work a new self-synchronized encryption method for 10 Gigabit optical links is proposed and developed. Necessary modifications to introduce this kind of encryption in physical layers based on 64b/66b encoding, such as 10GBase-R, have been considered. The proposed scheme encrypts directly the 64b/66b blocks by using a symmetric stream cipher based on an FPE (Format Preserving Encryption) block cipher operating in PSCFB (Pipelined Statistical Cipher Feedback) mode. One of the main novelties in this paper is the security analysis done for this mode. For the first time, an expression for the IND-CPA (Indistinguishability under Chosen-Plaintext Attack) advantage of any adversary over this scheme has been derived. Moreover, it has been concluded that this mode can be considered secure in the same way of traditional modes are. In addition, the overall system has been simulated and implemented in an FPGA (Field Programmable Gate Array). An encrypted optical link has been tested with Ethernet data frames, concluding that it is possible to cipher traffic at this level, getting maximum throughput and hiding traffic pattern from passive eavesdroppers

Self-Synchronized Encryption for Physical Layer in Gigabit Ethernet Optical Links

In this work a new self-synchronized symmetric encryption solution for high speed communication systems necessary to preserve the format of the plaintext is proposed, developed and tested. This new encryption mechanism is based on the block cipher operation mode called PSCFB (Pipelined Statistical Cipher Feedback) and the modulo operation. The confidentiality of this mode is analyzed in terms of its IND-CPA (Indistinguishability under Chosen-Plaintext Attack) advantage, concluding that it can be considered secure in the same way as traditional modes are. The encryption system has been integrated in the physical layer of a 1000Base-X Gigabit Ethernet Interface, where the 8b/10b symbol flow is encrypted at line rate. Moreover, an implementation of the proposed system has been carried out in an FPGA (Field Programmable Gate Array) device. Finally, an encrypted optical link has been tested with real Ethernet frames, getting maximum throughput and protecting the data traffic from passive eavesdroppers

Chaotic Encryption for 10-Gb Ethernet Optical Links

In this paper, a new physical layer encryption method for optical 10-Gb Ethernet links is proposed. Necessary modifications to introduce encryption in Ethernet 10GBase-R standard have been considered. This security enhancement has consisted of a symmetric streaming encryption of the 64b/66b data flow at physical coding sublayer level thanks to two keystream generators based on a chaotic algorithm. The overall system has been implemented and tested in a field programmable gate array. Ethernet traffic has been encrypted, transmitted, and decrypted over a multimode optical link. Experimental results are analyzed concluding that it is possible to cipher traffic at this level and hide the complete Ethernet traffic pattern from any passive eavesdropper. In addition, no overhead is introduced during encryption, getting no losses in the total throughput

Chaos-Based Bitwise Dynamical Pseudorandom Number Generator on FPGA

In this paper, a new pseudorandom number generator (PRNG) based on the logistic map has been proposed. To prevent the system to fall into short period orbits as well as increasing the randomness of the generated sequences, the proposed algorithm dynamically changes the parameters of the chaotic system. This PRNG has been implemented in a Virtex 7 field-programmable gate array (FPGA) with a 32-bit fixed point precision, using a total of 510 lookup tables (LUTs) and 120 registers. The sequences generated by the proposed algorithm have been subjected to the National Institute of Standards and Technology (NIST) randomness tests, passing all of them. By comparing the randomness with the sequences generated by a raw 32-bit logistic map, it is shown that, by using only an additional 16% of LUTs, the proposed PRNG obtains a much better performance in terms of randomness, increasing the NIST passing rate from 0.252 to 0.989. Finally, the proposed bitwise dynamical PRNG is compared with other chaos-based realizations previously proposed, showing great improvement in terms of resources and randomness

In vitro and in vivo anti-Trypanosoma cruzi activity of new arylamine Mannich base-type derivatives

Chagas disease is a neglected tropical disease with 6-7 million people infected worldwide and there is no effective treatment. Therefore, there is an urgent need to continue researching in order to discover novel therapeutic alternatives. We present a series of arylaminoketone derivatives as means of identifying new drugs to treat Chagas disease in the acute phase with greater activity, less toxicity and with a larger spectrum of action than that corresponding to the reference drug benznidazole. Indexes of high selectivity found in vitro formed the basis for later in vivo assays in BALB/c mice. Murine model results show that compounds 3, 4, 7 and 10 induced a remarkable decrease in parasitemia levels in acute phase and the parasitemia reactivation following immunosuppression, and curative rates were higher than with benznidazole. These high anti-parasitic activities encourage us to propose these compounds as promising molecules for developing an easy to synthesize anti-Chagas agent

In vitro and in vivo anti-Trypanosoma cruzi activity of new arylamine Mannich base-type derivatives

Chagas disease is a neglected tropical disease with 6-7 million people infected worldwide and there is no effective treatment. Therefore, there is an urgent need to continue researching in order to discover novel therapeutic alternatives. We present a series of arylaminoketone derivatives as means of identifying new drugs to treat Chagas disease in the acute phase with greater activity, less toxicity and with a larger spectrum of action than that corresponding to the reference drug benznidazole. Indexes of high selectivity found in vitro formed the basis for later in vivo assays in BALB/c mice. Murine model results show that compounds 3, 4, 7 and 10 induced a remarkable decrease in parasitemia levels in acute phase and the parasitemia reactivation following immunosuppression, and curative rates were higher than with benznidazole. These high anti-parasitic activities encourage us to propose these compounds as promising molecules for developing an easy to synthesize anti-Chagas agent

Redox proteins of hydroxylating bacterial dioxygenases establish a regulatory cascade that prevents gratuitous induction of tetralin biodegradation genes

Bacterial dioxygenase systems are multicomponent enzymes that catalyze the initial degradation of many environmentally hazardous compounds. In Sphingopyxis granuli strain TFA tetralin dioxygenase hydroxylates tetralin, an organic contaminant. It consists of a ferredoxin reductase (ThnA4), a ferredoxin (ThnA3) and a oxygenase (ThnA1/ThnA2), forming a NAD(P)H–ThnA4–ThnA3–ThnA1/ThnA2 electron transport chain. ThnA3 has also a regulatory function since it prevents expression of tetralin degradation genes (thn) in the presence of non-metabolizable substrates of the catabolic pathway. This role is of physiological relevance since avoids gratuitous and wasteful production of catabolic enzymes. Our hypothesis for thn regulation implies that ThnA3 exerts its action by diverting electrons towards the regulator ThnY, an iron-sulfur flavoprotein that together with the transcriptional activator ThnR is necessary for thn gene expression. Here we analyze electron transfer among ThnA4, ThnA3 and ThnY by using stopped-flow spectrophotometry and determination of midpoint reduction potentials. Our results indicate that when accumulated in its reduced form ThnA3 is able to fully reduce ThnY. In addition, we have reproduced in vitro the regulatory circuit in the proposed physiological direction, NAD(P)H–ThnA4–ThnA3–ThnY. ThnA3 represents an unprecedented way of communication between a catabolic pathway and its regulatory system to prevent gratuitous induction

Validation of the protein kinase PfCLK3 as a multistage cross-species malarial drug target

The requirement for next-generation antimalarials to be both curative and transmission-blocking necessitates the identification of previously undiscovered druggable molecular pathways. We identified a selective inhibitor of the Plasmodium falciparum protein kinase PfCLK3, which we used in combination with chemogenetics to validate PfCLK3 as a drug target acting at multiple parasite life stages. Consistent with a role for PfCLK3 in RNA splicing, inhibition resulted in the down-regulation of more than 400 essential parasite genes. Inhibition of PfCLK3 mediated rapid killing of asexual liver- and blood-stage P. falciparum and blockade of gametocyte development, thereby preventing transmission, and also showed parasiticidal activity against P. berghei and P. knowlesi Hence, our data establish PfCLK3 as a target for drugs, with the potential to offer a cure-to be prophylactic and transmission blocking in malaria