Surface magnetism in ZnO/Co3O4 mixtures

We recently reported the observation of room temperature ferromagnetism in mixtures of ZnO and Co3O4 despite the diamagnetic and antiferromagnetic character of these oxides respectively. Here we present a detailed study on the electronic structure of this material in order to account for this unexpected ferromagnetism. Electrostatic interactions between both oxides lead to a dispersion of Co3O4 particles over the surface of ZnO larger ones. As a consequence, the reduction of Co+3 to Co2+ at the particle surface takes place as evidenced by XAS measurements and optical spectrocopy. This reduction allows to xplain the observed ferromagnetic signal within the well established theories of magnetism.Comment: Accepted in Journal of Applied Physic

SPH simulations of thixo-viscoplastic fluid flow past a cylinder

Thixotropic materials are complex fluids that display time-dependent viscosity and/or yield-stress response upon the application of a fixed deformation, while recovering their original structured-state when the deformation is discontinued. Thixotropic effects are presents in many different systems and applications, ranging from food products, such as ketchup, to metals, such as molten aluminum. In this work we present a first attempt to simulate the rheological properties of thixo-viscoplastic flows using a Smoothed Particle Hydrodynamic (SPH) method. The study set up is a 2D flow around a circular cylinder as well as a simple shear flow between parallel plates to validate our numerical results. SPH solutions are compared with simulations performed using the open-source Finite Volume Method solver RheoTool, based on OpenFOAM. The viscoplastic model used in this work is the Papanastasiou model combined with a recently developed microstructural one, in order to include thixotropy. In this thixo-viscoplastic framework, we analyze the flow properties in terms of yield-fronts, streamlines and structure-parameter fields at different Bingham and Thixotropy numbers, through microstructural thixotropic and yield-stress parameters variation. Obtained results show an important novelty: an asymmetry in the thixo-viscoplastic flow around the cylinder

Photo-Fenton oxidation of cylindrospermopsin at neutral pH with LEDs

Cylindrospermopsin (CYN) is a potent cyanobacterial toxin found in freshwaters worldwide. In this work, the feasibility of the photo-Fenton process under neutral pH using light emitting diodes as irradiation source for the removal of this hazardous cyanotoxin from freshwater was investigated. The impact of the kind of iron chelating agent (ethylenediamine-N, N′-disuccinic acid vs. ethylenedinitrilotetraacetic acid) as well as the effect of the main operating conditions viz. H2O2 dose, Fe(III) load, initial CYN concentration, and Fe(III):EDDS molar ratio on the performance of the process was systematically evaluated. EDDS was selected as the most appropriate iron chelating agent considering the kinetics of the process and the environmental impact (Vibrio fischeri and Artemia salina). Under optimized conditions ([H2O2] = 30 mg L−1; [Fe(III)] = 5 mg L−1; Fe(III):ligand = 1:0.5 (molar ratio)), complete removal of CYN was achieved in 15-min reaction time. Furthermore, the catalytic system showed to be effective in real water matrices (river and reservoir waters) spiked with CYN. Although the presence of inorganic ions (mainly HCO3−/CO32−) and dissolved organic carbon decreased the oxidation rate of CYN due to scavenging reactions and iron coordination, respectively, complete elimination of the cyanotoxin was achieved in all cases. The fate of EDDS along the process was also evaluated to demonstrate that the catalytic system investigated, apart from its effectiveness, warrants the complete absence of residues after reaction. Therefore, the proposed system constitutes a promising method for cyanotoxin treatment either as a drinking water treatment step in conventional plants or as a potential remediation strategy in the natural environmentOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research has been supported by the CYTED Ibero-American Science and Technology Program for Development (CYTED-2019) through the project TALGENTOX (PCI2020-112013) and by the Spanish Ministry for Science and Innovation (MICINN) through the project PID2019-105079RB-I00. D. Ortiz has received a FPU predoctoral grant (FPU19/04816) from the Spanish Ministry of Universities. M. Munoz has received a Ramón y Cajal postdoctoral contract (RYC-2016–20648) from the Spanish MINEC

Analysis of Finite Microstrip Structures Using an Efficient Implementation of the Integral Equation Technique

An efficient numerical implementation of the Integral Equation technique (IE) has been developed for the analysis of the electrical characteristics of finite microstrip structures. The technique formulates a volume version of the IE for the finite dielectric objects, and a standard surface IE technique for the metallic areas. The system of integral equations formu- lated are solved with special numerical techniques described in this paper. The input impedances of several microstrip antennas have been computed, showing good agreement with respect mea- surements. The technique has shown to be accurate even for complex geometries containing several stacked dielectric layers. The radiation patterns of the structures have also been com- puted, and measured results from real manufactured hardware confirm that backside radiation and secondary lobes are accurately predicted by the theoretical model. The paper also discuss a suitable excitation model for finite size ground planes, and investigates the possibilities for an independent meshing of the metallic areas and the dielectric objects inside a given geom- etry. The practical value of the approach derived is that microstrip circuits can be designed minimizing the volume and size of the dielectric substrates.This work has been supported bythe Spanish National Project ESP2001-4546-PE, and RegionalSeneca Project PB/4/FS/02

Sources of experimental errors in the observation of nanoscale magnetism

It has been recently reported that some non-magnetic materials in bulk state, exhibit magnetic behavior at the nanscale due to surface and size effects. The experimental observation of these effects is based on the measurement of very small magnetic signals. Thus, some spurious effects that are not critical for bulk materials with large magnetic signals may become important when measuring small signals (typically below 0.0001 emu). Here, we summarize some sources of these small magnetic signals that should be considered when studying this new nanomagnetismComment: 16 pages, 10 figure

Which came first: The disease or the pest? Is there a host mediated spread of Beauveria bassiana (Ascomycota: Hypocreales) by invasive palm pests?

The red palm weevil (RPW) Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) is threatening the palm family worldwide, causing important economic losses. Current tactics to manage the weevil are largely based on chemical control, although the use of pesticides is hampered by several environmental constraints. Since the first introduction of RPW in Spain in 1996 and during its progressive spread around the Mediterranean basin, the number of reports of natural infection of RPW populations by entomopathogenic fungi (EPF) has been rising for 15 years, and this rise could support a pest-mediated EPF spread. To challenge this hypothesis, we assessed the usefulness of the region of elongation factor 1-alpha (EF1-alpha), Bloc nuclear intergenic region (Bloc) and inter simple sequence repeat (ISSR) markers, alone or in combination, to infer the relationships among Mediterranean Beauveria and Metarhizium strains isolated from the RPW. Second, the effect of abiotic factors, such as temperature, humidity and UV-B radiation, on the germination and growth of these EPFs strains as a function of their genealogy and geographic origin were determined. Finally, the pathogenicity of strains from different genetic clades was evaluated against larvae and adults of R. ferrugineus. The phylogenetic analysis based on the EF-1 alpha gene identified eight different sequences among 24 fungal isolates of four fungal species. Similar clades were clustered when Bloc and ISSR analyses were performed. The results showed that strains of different origins were clustered in the same Glade, and this outcome could be explained by an RPW-mediated EPF spread that was also influenced by time, geographical and other RPW related factors. Neither the response to abiotic factors nor virulence to RPW larvae and adults were related to the sequence type, with all B. bassiana strains well adapted to Mediterraneam climatic conditions. Taken together, these findings may help to select the best strain for RPW management

Lifestyles, arterial aging, and its relationship with the intestinal and oral microbiota (MIVAS III study): a research protocol for a cross-sectional multicenter study

The microbiota is increasingly recognized as a significant factor in the pathophysiology of many diseases, including cardiometabolic diseases, with lifestyles probably exerting the greatest influence on the composition of the human microbiome. The main objectives of the study are to analyze the association of lifestyles (diet, physical activity, tobacco, and alcohol) with the gut and oral microbiota, arterial aging, and cognitive function in subjects without cardiovascular disease in the Iberian Peninsula. In addition, the study will examine the mediating role of the microbiome in mediating the association between lifestyles and arterial aging as well as cognitive function.Methods and analysisMIVAS III is a multicenter cross-sectional study that will take place in the Iberian Peninsula. One thousand subjects aged between 45 and 74 years without cardiovascular disease will be selected. The main variables are demographic information, anthropometric measurements, and habits (tobacco and alcohol). Dietary patterns will be assessed using a frequency consumption questionnaire (FFQ) and the Mediterranean diet adherence questionnaire. Physical activity levels will be evaluated using the International Physical Activity Questionnaire (IPAQ), Marshall Questionnaire, and an Accelerometer (Actigraph). Body composition will be measured using the Inbody 230 impedance meter. Arterial aging will be assessed through various means, including measuring medium intimate carotid thickness using the Sonosite Micromax, conducting analysis with pulse wave velocity (PWA), and measuring pulse wave velocity (cf-PWV) using the Sphygmocor System. Additional cardiovascular indicators such as Cardio Ankle Vascular Index (CAVI), ba-PWV, and ankle-brachial index (Vasera VS-2000®) will also be examined. The study will analyze the intestinal microbiota using the OMNIgene GUT kit (OMR−200) and profile the microbiome through massive sequencing of the 16S rRNA gene. Linear discriminant analysis (LDA), effect size (LEfSe), and compositional analysis, such as ANCOM-BC, will be used to identify differentially abundant taxa between groups. After rarefying the samples, further analyses will be conducted using MicrobiomeAnalyst and R v.4.2.1 software. These analyses will include various aspects, such as assessing α and β diversity, conducting abundance profiling, and performing clustering analysis.DiscussionLifestyle acts as a modifier of microbiota composition. However, there are no conclusive results demonstrating the mediating effect of the microbiota in the relationship between lifestyles and cardiovascular diseases. Understanding this relationship may facilitate the implementation of strategies for improving population health by modifying the gut and oral microbiota

Genetic study of atypical femoral fractures using exome sequencing in three affected sisters and three unrelated patients

Objectives: Atypical femoral fractures (AFF) are rare, often related to long-term bisphosphonate (BPs) tre- atment. Their pathogenic mechanisms are not precisely known and there is no evidence to identify patients with a high risk of AFF. The aim of this work is to study the genetic bases of AFFs. Material and methods: Whole-exome sequencing was carried out on 3 sisters and 3 unrelated additional patients, all treated with BPs for more than 5 years. Low frequency, potentially pathogenic variants sha- red by the 3 sisters, were selected, were selected and a network of gene and protein interactions was constructed with the data found. Results: We identified 37 rare variants (in 34 genes) shared by the 3 sisters, some not previously descri- bed. The most striking variant was the p.Asp188Tyr mutation in the enzyme geranylgeranyl pyrophos- phate synthase (encoded by the GGPS1 gene), from the mevalonate pathway and essential for osteoclast function. Another noteworthy finding was two mutations (one in the 3 sisters and one in an unrelated patient) in the CYP1A1 gene, involved in the metabolism of steroids. We identified other variants that could also be involved in the susceptibility to AFFs or in the underlying osteoporotic phenotype, such as those present in the SYDE2, NGEF, COG4 and FN1 genes. Conclusions: Our data are compatible with a model where the accumulation of susceptibility variants could participate in the genetic basis of AFFs

Efectos de la entrevista motivacional en la salud oral de pacientes pediátricos

Objetivo: determinar la efectividad a corto plazo de una intervención basada en la entrevista motivacional (EM). en la reducción del índice de placa bacteriana en niños de entre 7 y 12 años (segunda infancia), en comparación con el modelo informativo tradicional (MIT). Métodos y diseño: con este objetivo fue realizado un ensayo clínico aleatorizado a doble ciego, con 30 pacientes y dos grupos activos de intervención. Al inicio del estudio se examinaron todos los participantes en relación con el índice de placa bacteriana (O'Leary) y. posteriormente, fueron asignados de fomia aleatoria al grupo experimental (una sola sesión EM) y al gnipo control (una sola sesión MIT). ambas con una duración aproximada de 15 minutos. A excepción de esta sesión, todos los participantes siguieron el mismo protocolo de tratamiento. Resultados: a los 3 meses postintervención, la placa bacteriana determinada por el índice de O'Leary disminuyó en ambos grupos (76. l frente a 69.8^f). con una diferencia altamente significativa para p 0,05 (t = 0,78; p = 0,441). Conclusiones: desde una perspectiva a corto plazo, una sesión breve de EM logró reducir los índices de placa bacteriana en pacientes infantiles, pero no demostró ser más eficaz que el MIT

Pranlukast Antagonizes CD49f and Reduces Sternness in Triple-Negative Breast Cancer Cells

Introduction: Cancer stem cells (CSCs) drive the initiation, maintenance, and therapy response of breast tumors. CD49f is expressed in breast CSCs and functions in the maintenance of stemness. Thus, blockade of CD49f is a potential therapeutic approach for targeting breast CSCs. In the present study, we aimed to repurpose drugs as CD49f antagonists. Materials and Methods: We performed consensus molecular docking using a subdomain of CD49f that is critical for heterodimerization and a collection of pharmochemicals clini-cally tested. Molecular dynamics simulations were employed to further characterize drug-target binding. Using MDA-MB-231 cells, we evaluated the effects of potential CD49f antagonists on 1) cell adhesion to laminin; 2) mammosphere formation; and 3) cell viability. We analyzed the effects of the drug with better CSC-selectivity on the activation of CD49f-downstream signaling by Western blot (WB) and co-immunoprecipitation. Expressions of the stem cell markers CD44 and SOX2 were analyzed by flow cytometry and WB, respectively. Transactivation of SOX2 promoter was evaluated by luciferase reporter assays. Changes in the number of CSCs were assessed by limiting-dilution xenotransplantation. Results: Pranlukast, a drug used to treat asthma, bound to CD49f in silico and inhibited the adhesion of CD49f+ MDA-MB-231 cells to laminin, indicating that it antagonizes CD49f-containing integrins. Molecular dynamics analysis showed that pranlukast binding induces con-formational changes in CD49f that affect its interaction with β1-integrin subunit and constrained the conformational dynamics of the heterodimer. Pranlukast decreased the clonogenicity of breast cancer cells on mammosphere formation assay but had no impact on the viability of bulk tumor cells. Brief exposure of MDA-MB-231 cells to pranlukast altered CD49f-dependent signaling, reducing focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3K) activation. Further, pranlukast-treated cells showed decreased CD44 and SOX2 expression, SOX2 promoter transacti-vation, and in vivo tumorigenicity, supporting that this drug reduces the frequency of CSC. Conclusion: Our results support the function of pranlukast as a CD49f antagonist that reduces the CSC population in triple-negative breast cancer cells. The pharmacokinetics and toxicology of this drug have already been established, rendering a potential adjuvant therapy for breast cancer patients