The MHC-encoded TAP1/LMP2 bidirectional promoter is down-regulated in highly oncogenic adenovirus type 12 transformed cells

AbstractCells transformed by human adenovirus 12 (Ad12) exhibit extremely low surface levels of MHC class I molecules and contain reduced levels of class I heavy chain mRNAs. We report that levels of MHC-encoded TAP1 and LMP2 mRNAs are also down-regulated in Ad12-transformed rat cells, and that transcription of rat TAP1 and LMP2 transcripts is directed from a 564 bp intergenic region which is significantly less active in Ad12-transformed cells compared to those transformed with Ad5. Our results suggest that, in common with MHC class I gene expression, TAP1 and LMP2 gene expression is reduced mainly at the level of transcription in Ad12-transformed cells

Temporal trends and transport within and around the Antarctic polar vortex during the formation of the 1987 Antarctic ozone hole

During AAOE in 1987 an ER-2 high altitude aircraft made twelve flights out of Punta Arenas, Chile (53 S, 71 W) into the Antarctic polar vortex. The aircraft was fitted with fast response instruments for in situ measurements of many trace species including O3, ClO, BrO, NO sub y, NO, H2O, and N2O. Grab samples of long-lived tracers were also taken and a scanning microwave radiometer measured temperatures above and below the aircraft. Temperature, pressure, and wind measurements were also made on the flight tracks. Most of these flights were flown to 72 S, at a constant potential temperature, followed by a dip to a lower altitude and again assuming a sometimes different potential temperature for the return leg. The potential temperature chosen was 425 K (17 to 18 km) on 12 of the flight legs, and 5 of the flight legs were flown at 450 K (18 to 19 km). The remaining 7 legs of the 12 flights were not flown on constant potential temperature surfaces. Tracer data have been analyzed for temporal trends. Data from the ascents out of Punta Arenas, the constant potential temperature flight legs, and the dips within the vortex are used to compare tracer values inside and outside the vortex, both with respect to constant potential temperature and constant N2O. The time trend during the one-month period of August 23 through September 22, 1987, shows that ozone decreased by 50 percent or more at altitudes form 15 to 19 km. This trend is evident whether analyzed with respect to constant potential temperature or constant N2O. The trend analysis for ozone outside the vortex shows no downward trend during this period. The analysis for N2O at a constant potential temperature indicates no significant trend either inside or outside the vortex; however, a decrease in N2O with an increase in latitude is evident

K-shell photoionization of ground-state Li-like boron ions [B2+^{2+}]: Experiment and Theory

Absolute cross sections for the K-shell photoionization of ground-state Li-like boron [B$^{2+}$(1s$^2$2s $^2$S)] ions were measured by employing the ion-photon merged-beams technique at the Advanced Light Source synchrotron radiation facility. The energy ranges 197.5--200.5 eV, 201.9--202.1 eV of the [1s(2s\,2p)$^3$P]$^2$P${\rm ^o}$ and [1s(2s\,2p)$^1$P] $^2$P${\rm ^o}$ resonances, respectively, were investigated using resolving powers of up to 17\,600. The energy range of the experiments was extended to about 238.2 eV yielding energies of the most prominent [1s(2$\ell$\,n$\ell^{\prime}$)]$^2$P$^o$ resonances with an absolute accuracy of the order of 130 ppm. The natural linewidths of the [1s(2s\,2p)$^3$P] $^2$P${\rm ^o}$ and [1s(2s\,2p)$^1$P] $^2$P${\rm ^o}$ resonances were measured to be $4.8 \pm 0.6$ meV and $29.7 \pm 2.5$ meV, respectively, which compare favourably with theoretical results of 4.40 meV and 30.53 meV determined using an intermediate coupling R-matrix method.Comment: 6 figures and 2 table

M67-1194, an unusually Sun-like solar twin in M67

The rich open cluster M67 is known to have a chemical composition close to solar, and an age around 4Gyr. It thus offers the opportunity to check our understanding of the physics and the evolution of solar-type stars in a cluster environment. We present the first spectroscopic study at high resolution, R~50,000, of the potentially best solar twin, M67-1194, identified among solar-like stars in M67. Based on a pre-selection of solar-twin candidates performed at medium resolution by Pasquini et al. (2008), we explore the chemical-abundance similarities and differences between M67-1194 and the Sun, using VLT/FLAMES-UVES. Working with a solar twin in the framework of a differential analysis, we minimize systematic model errors in the abundance analysis compared to previous studies which utilized more evolved stars to determine the metallicity of M67. We find M67-1194 to have stellar parameters indistinguishable from the solar values, with the exception of the overall metallicity which is slightly super-solar ([Fe/H]=0.023 +/- 0.015). An age determination based on evolutionary tracks yields 4.2 +/- 1.6Gyr. Most surprisingly, we find the chemical abundance pattern to closely resemble the solar one, in contrast to most known solar twins in the solar neighbourhood. We confirm the solar-twin nature of M67-1194, the first solar twin known to belong to a stellar association. This fact allows us to put some constraints on the physical reasons for the seemingly systematic departure of M67-1194 and the Sun from most known solar twins regarding chemical composition. We find that radiative dust cleansing by nearby luminous stars may be the explanation for the peculiar composition of both the Sun and M67-1194, but alternative explanations are also possible. The chemical similarity between the Sun and M67-1194 also suggests that the Sun once formed in a cluster like M67

Detailed Analysis of Nearby Bulgelike Dwarf Stars II. Lithium Abundances

Li abundances are derived for a sample of bulgelike stars with isochronal ages of 10-11 Gyr. These stars have orbits with pericentric distances, Rp, as small as 2-3 kpc and Zmax < 1 kpc. The sample comprises G and K dwarf stars in the metallicity range -0.80<[Fe/H]< +0.40. Few data of Li abundances in old turn-off stars (> 4.5 Gyr) within the present metallicity range are available. M67 (4.7 Gyr) and NGC 188 (6 Gyr) are the oldest studied metal-rich open clusters with late-type stars. Li abundances have also been studied for few samples of old metal-rich field stars. In the present work a high dispersion in Li abundances is found for bulgelike stars for all the metallicity range, comparable with values in M67. The role of metallicity and age on a Li depletion pattern is discussed. The possible connection between Li depletion and oxygen abundance due to atmospheric opacity effects is investigated.Comment: 9 pages, 7 figure

Testing Rotational Mixing Predictions with New Boron Abundances in Main Sequence B-type Stars

(Abridged) New boron abundances for seven main-sequence B-type stars are determined from HST STIS spectroscopy around the BIII 2066A line. Boron abundances provide a unique and critical test of stellar evolution models that include rotational mixing since boron is destroyed in the surface layers of stars through shallow mixing long before other elements are mixed from the stellar interior through deep mixing. Boron abundances range from 12+log(B/H) = 1.0 to 2.2. The boron abundances are compared to the published values of their stellar nitrogen abundances (all have 12+log(N/H) < 7.8, i.e., they do not show significant CNO-mixing) and to their host cluster ages (4 to 16 Myr) to investigate the predictions from models of massive star evolution with rotational mixing effects (Heger & Langer 2000). Only three stars (out of 34) deviate from the model predictions, including HD36591, HD205021, and HD30836. These three stars suggest that rotational mixing could be more efficient than currently modelled at the highest rotation rates.Comment: 10 figures, 7 tables; accepted for publication in the Astrophysical Journa

Spectroscopic Sensitivity

We describe the overall performance of the STIS CCD after HST Servicing Mission #4 and the associated updates to calibration reference files. Most aspects of CCD performance are found to be fairly consistent with extrapolations of the trends seen prior to the failure of STIS in August 2004. The CCD gain value for the CCDGAIN = 4 setting has been redetermined using net count ratios of standard star spectra taken in the CCDGAIN = 1 and CCDGAIN = 4 settings, resulting in a gain value of 4.016 ± 0.003 e − /DN, which is 0.5% lower than the value used for the calibration of archival STIS CCD data taken before August 2004. Finally, we identify two independent indications of a temperature dependence of the Charge Transfer Efficiency (CTE). However, more calibration data are needed to verify the significance of this effect and, if verified, to calibrate it as a function of CCD housing temperature (as a proxy for CCD chip temperature). This option will be reassessed later during the Cycle 17 calibration program.

Limits on the Optical Brightness of the Epsilon Eridani Dust Ring

The STIS/CCD camera on the {\em Hubble Space Telescope (HST)} was used to take deep optical images near the K2V main-sequence star $\epsilon$ Eridani in an attempt to find an optical counterpart of the dust ring previously imaged by sub-mm observations. Upper limits for the optical brightness of the dust ring are determined and discussed in the context of the scattered starlight expected from plausible dust models. We find that, even if the dust is smoothly distributed in symmetrical rings, the optical surface brightness of the dust, as measured with the {\em HST}/STIS CCD clear aperture at 55 AU from the star, cannot be brighter than about 25 STMAG/"$^2$. This upper limit excludes some solid grain models for the dust ring that can fit the IR and sub-mm data. Magnitudes and positions for $\approx$59 discrete objects between 12.5" to 58" from $\epsilon$ Eri are reported. Most if not all of these objects are likely to be background stars and galaxies.Comment: Revision corrects author lis

Iron abundances from optical Fe III absorption lines in B-type stellar spectra

The role of optical Fe III absorption lines in B-type stars as iron abundance diagnostics is considered. To date, ultraviolet Fe lines have been widely used in B-type stars, although line blending can severely hinder their diagnostic power. Using optical spectra, covering a wavelength range ~ 3560 - 9200 A, a sample of Galactic B-type main-sequence and supergiant stars of spectral types B0.5 to B7 are investigated. A comparison of the observed Fe III spectra of supergiants, and those predicted from the model atmosphere codes TLUSTY (plane-parallel, non-LTE), with spectra generated using SYNSPEC (LTE), and CMFGEN (spherical, non-LTE), reveal that non-LTE effects appear small. In addition, a sample of main-sequence and supergiant objects, observed with FEROS, reveal LTE abundance estimates consistent with the Galactic environment and previous optical studies. Based on the present study, we list a number of Fe III transitions which we recommend for estimating the iron abundance from early B-type stellar spectra.Comment: 3 figures and 8 tables. Table 3 is to be published online only (included here on last page). Accepted for publication in MNRA

Turbulent spectrum of the Earth's ozone field

The Total Ozone Mapping Spectrometer (TOMS) database is subjected to an analysis in terms of the Karhunen-Loeve (KL) empirical eigenfunctions. The concentration variance spectrum is transformed into a wavenumber spectrum, $E_c(k)$. In terms of wavenumber $E_c(k)$ is shown to be $O(k^{-2/3})$ in the inverse cascade regime, $O(k^{-2})$ in the enstrophy cascade regime with the spectral {\it knee} at the wavenumber of barotropic instability.The spectrum is related to known geophysical phenomena and shown to be consistent with physical dimensional reasoning for the problem. The appropriate Reynolds number for the phenomena is $Re\approx 10^{10}$.Comment: RevTeX file, 4 pages, 4 postscript figures available upon request from Richard Everson <[email protected]