The profiling of short tandem repeats (STRS) fromnext-generation sequencing (NGS) data by establishing a whole genome STRS pipeline for forensic bioinformatics

Repeat region with length of one to six base pairs (bp), found in DNA sequences is known as short tandem repeats (STRs). Currently, high-throughput next-generation sequencers have facilitated the effective polymorphic STR markers’ identification. In this study, a new whole genome STRs pipeline has been established in order to call and profile STRs from next-generation sequencing (NGS) data. Firstly, genome sequences of Helicobater pylori strain, CPY1124 and PeCan4 as reference genome were retrieved from European Nucleotide Archive (ENA) database which then the quality of sequences were checked using FastQC. The assembly of genome sequences was done by VELVET de novo assembler. Unordered contigs from VELVET’s output was realigned using multiple genome alignment (MAUVE) to obtain ordered contigs sequence. Lastly, STRs calling and profiling by Tandem Repeat Finder was done with the parameters of (2: match, 7: mismatch and 7: indels). These parameters are for Smith-Waterman style local alignment using wrap-around dynamic programming. As a result, this new pipeline enables to identify polymorphic and unique STRs which are GTTTG and AAACCC from CPY1124. This pipeline has been compared with other available STRs profiling pipeline like pSTR Finder and Tandem Repeat Database (TRDB) for validation purpose. The similar output producing by both tools thus indicates the reliability of this new pipeline for future usage

Pengalaman Komunikasi Terapeutik Perawat Orang Lanjut Usia

Although some research related to the influence of therapeutic communication to patients has been carried out in Indonesia, research trying to explore the experience of the elderly nurse communication has never been done. Though the geriatric is a practitioner who has interacted daily with elderly patients thus have therapeutic communication experience that deserves to be explored. By using phenomenology, the study describe the experience of five geriatric nurse in conducting therapeutic communication activities for elderly patients in Sasana Tresna Werdha Jakarta. Data was collected through interviews. The informants of this research are interviewed about 45-90 minutes. In the interview the informant's provide their personal experience that contribute to the success of their therapeutic communication. Based on this study, we found ten important themes in therapeutic communicate with elderly patients, namely: the planned communication, to find out, observation, appreciation and humor, taboo communication, respect, caring, clarity, empathy and build rapport. The ten themes above we made to three (3) things that become the main learning and the essence in this study. 3 (three) it was planned, caring, and empathy are the answer to the research question

Sequestering atmospheric CO₂ inorganically:a solution for Malaysia's CO₂ emission

Malaysia is anticipating an increase of 68.86% in CO2 emission in 2020, compared with the 2000 baseline, reaching 285.73 million tonnes. A major contributor to Malaysia's CO2 emissions is coal-fired electricity power plants, responsible for 43.4% of the overall emissions. Malaysia's forest soil offers organic sequestration of 15 tonnes of CO2 ha(-1) year(-1). Unlike organic CO2 sequestration in soil, inorganic sequestration of CO2 through mineral carbonation, once formed, is considered as a permanent sink. Inorganic CO2 sequestration in Malaysia has not been extensively studied, and the country's potential for using the technique for atmospheric CO2 removal is undefined. In addition, Malaysia produces a significant amount of solid waste annually and, of that, demolition concrete waste, basalt quarry fine, and fly and bottom ashes are calcium-rich materials suitable for inorganic CO2 sequestration. This project introduces a potential solution for sequestering atmospheric CO2 inorganically for Malaysia. If lands associated to future developments in Malaysia are designed for inorganic CO2 sequestration using demolition concrete waste, basalt quarry fine, and fly and bottom ashes, 597,465 tonnes of CO2 can be captured annually adding a potential annual economic benefit of (sic)4,700,000.</p

Simulated data of urea of haemodialysis patient using one-compartment model

End stage kidney disease (ESKD) is a disease where both kidneys are no longer functioning properly and patients require renal replacement therapy (RRT) such as haemodialysis to prolong their life. The numbers of modality for haemodialysis had increased, as the numbers of new patient of ESKD increased. In Malaysia, the numbers of new patient had increased three-fold for the past 10 years, from year 2002 to year 2012. In this paper, demographic data, clinical data and input and output of dialysis for the selected patients are presented. The post-dialysis urea concentrations for each patient with different weightfor one compartment model were simulated using MATLAB software. The simulated values of post-dialysis urea concentration were then compared with the clinical values of post-dialysis urea concentration for different weight. The simulated values of post-dialysis urea concentration underestimated the clinical value of post-dialysis urea concentration for patient with weight less than 70kg but overestimated to patient with weight greater than 70kg. The ratio of simulated values and clinical values of post-dialysis urea concentration were found to increase with the weight of patien

Correlation Between BMI and ADLs with Mental Emotional Disorder Among Elderly

The increasing of life expectancy led to a growing number of elderly. There are many thing that occurs caused by aging process, one of them is decreases of body function. Decreasing body function can cause variety of health problems. The problem that may occurs are malnutrition, chronic disease, and lack of independence in performing daily activities. All of them are risk factor of mental emotional disorder. This study was conducted to determined the relationship between mental emotional disorders in the Elderly Care Unit Jombang in Kediri. This research was analytical study and used cross sectional. Dependent variable was mental emotional disorders. Independent variables were BMI, physical independence and characteristic. Interview was conducted to obtain information about variables in this study. Data analyzed by chi square and pearson correlation. The prevalence of mental disorders found in this study was 23,4%. Respondents who didn't have physical independence were 4,3%. Respondents who had body mass index that wasn't normal is 26,2%. The variables showed correlation only variable physical independence and mental emotional disorders (p = 0,008). While the body mass index and characteristic were not showed correlation with mental emotional disorders. The conclusion of this study was the elderly at risk for emotional suffering mental disorders. Physical independence associated with mental disorders in elderly emotional. So the need for more attention especially from the psychological to the elderly who do not have physical independence

GAMBARAN PENGGUNAAN OBAT PADA PASIEN INFEKSI SALURAN PERNAPASAN AKUT DI KLINIK PRATAMA ASTY SUKOHARJO

Acute Respiratory Infection (ARI) is an acute infectious disease that attacks one or more parts of the respiratory tract from the nose (upper tract) to the alveoli (lower tract). The primary therapy for ARI patients is antibiotic therapy and supportive therapy. This study aims to determine the use of drugs in patients with acute respiratory infections at the Pratama Asty Clinic Sukoharjo. The research is descriptive and data collection is done retrospectively. Sampling used the slovin formula with 95 medical records. This research was conducted in January-June 2021. Data analysis used descriptive analysis and presented in tables and percentages. The results showed that the use of drugs for acute respiratory infections at the Pratama Asty Clinic Sukoharjo the use of drugs based on the most widely used drug class, namely the antibiotic group, amounting to 21.82% with the type of antibiotic used the most, namely cefadroxil as much as 11.16%. The most widely used supporting therapy is the combination drug group with a total of 18.03% with the type of drug calortusin amounting to 6.85%. The multivitamin group was 18.03% with the type of multivitamin boost drug as much as 8.88%. The class of corticosteroid drugs was 18.28% and the type of dexamethasone was 9.40%. The conclusion of this study shows that the description of drug use in ARI patients at the Pratama Clinic Asty Sukoharjo using antibiotic therapy with the addition of supporting therapy as much as 90.5% and the results obtained are patients who only use supportive therapy without antibiotics as much as 9.5%

Program Pengembangan Usaha Kecil pada Sentra Industri Kecil Handycraft di Desa Tutul Kecamatan Balung Kabupaten Jember (Small Business Development Programs on the Center of Handicraft Small Industry in Tutul Village, Balung Sub-ditsrict, Jember Regency)

AbstractStarting from the existing problems related to human resources where the majority of the people of TutulVillage have a low educational background. Although in making handicrafts, formal education is not veryimportant, the low quality of the human resources is not supported by the development programs of skillstraining. The second problem is that small handicraft industry businessmen in Tutul village are stillconstrained by financial or capital resources, and the marketing process is still conventional, so the amountof production is still based on the number of orders coming. Therefore, this research aims to describe theimplementation of small business development programs in the center of handicraft small industry in Tutulvillage.This descriptive research employed a qualitative approach. The research data were collected throughobservation, documentation, and interview. The village head as themain informant was determined by usingsnowball sampling technique. To test thedata validity, the researchers conducted perseverance ofobservation and data triangulation. Data were analyzedby using qualitative data analysis by Miles andHuberman, namely data reduction, data presentation, and drawing conclusion. The findings indicated thatthe small business development programs in the center of handicraft small industry in Tutul village werethedevelopment programs of managerial aspect, especially human resource skills and marketing. The second,capital development programwas carried out by providing capital assistance to craftsmen by PT TelkomJember. The next program was through a partnership program that aimed to expand the network ofcraftsmen. The fourth program was the development of the center of small industry under the auspices ofTechnical Implementation Unit of Job Training Center (UPT BLK)Jember. The last development programwas coaching carried out by PT Telkom and UPT BLK Jember by conducting training and the assistance offacilities and infrastructures.Keywords: program, development, and small industr

Analisis Pengaruh Fenomena El Nino dan La Nina terhadap Curah Hujan Tahun 1998 - 2016 Menggunakan Indikator Oni (Oceanic Nino Index) (Studi Kasus : Provinsi Jawa Barat)

Perubahan iklim dalam rentang 10 tahun terakhir membawa Perubahan yang sangat drastis di permukaan bumi. Beberapa pengaruh iklim ini salah satunya anomali suhu udara yang mencolok seperti fenomena El Nino dan La Nina atau yang lebih dikenal dengan fenomena ENSO (El Nino Southern Oscillation). Fenomena ENSO merupakan suatu kondisi permukaan laut di wilayah Samudera Pasifik mengalami kenaikan atau penurunan suhu permukaan laut sehingga menyebabkan adanya pergeseran musim di wilayah Indonesia. Pergeseran musim yang terjadi karena fenomena ENSO juga berpengaruh besar terhadap produksi pangan dan komoditas pertanian yang lain.Pada penelitian ini, metode pengolahan data penelitian menggunakan bahasa pemograman untuk mengolah data SST dan data curah hujan dari tahun 1998 sampai tahun 2016. Data yang digunakan berupa data suhu permukaan laut yang berasal dari satelit NOAA yaitu SST Reynolds (Sea Surface Temperature) serta data curah hujan harian yang berasal dari satelit TRMM. Pembuatan indeks ONI pada penelitian ini menggunakan data SST bulanan yang telah dikonversikan kemudian dikelompokkan kedalam masing-masing kelas. Masing-masing kelas mempunyai nilai kurang dari -0,5 yaitu keadaan La Nina, lebih dari 0,5 yaitu keadaan El Nino dan nilai diantara -0,5 sampai 0,5 yaitu keadaan normal. Pengujian data menggunakan analisis pola spasial curah hujan dan suhu permukaan laut yang dipengaruhi oleh fenomena El Nino dan La Nina.Hasil penelitian berupa peta sebaran SST dan curah hujan secara musiman untuk mengetahui pengaruh dari fenomena El Nino dan La Nina di wilayah Jawa Barat. Fenomena El Nino dan La Nina di Laut Jawa terjadi pada bulan Agustus sampai bulan Februari. Pada saat El Nino, nilai suhu permukaan laut (SST) 27ᵒC -28ᵒC dengan rata-rata 27,71ᵒC sedangkan untuk intensitas curah hujannya yaitu 1,0mm/hr-2,0mm/hr dengan rata-rata 1,63mm/hr. Pada saat La Nina, nilai suhu permukaan laut (SST) 29ᵒC-30ᵒC dengan rata-rata 29,06ᵒC sedangkan intensitas curah hujannya yaitu 9,0mm/hr-10mm/hr dengan rata-rata 9,74mm/hr. Korelasi antara curah hujan dan SST sebesar 0,413 yang menyatakan hubungan yang cukup kuat antar parameter. Sehingga dapat disimpulkan, kenaikan SST saat La Nina mempengaruhi kenaikan intensitas curah hujan sedangkan untuk penurunan SST saat El Nino mempengaruhi penurunan intensitas curah hujan

Penyelenggaraan Pelayanan Administrasi Terpadu Kecamatan (Paten) Di Kabupaten Temanggung

Public service is one way to achieve general welfare. The implementation of public services effectively and efficiently is something that is expected by the public. Because today is the quality of public services in Indonesia is still far from good. In order to create a public service that is effective and efficient, the government made the Integrated Administrative Service District (PATEN).The aim of the study is to examine how the implementation of the Integrated Administrative Service District (PATEN) in Temanggung Regency, and identify factors inhibiting the implementation and to know the efforts that have been made by the government to improve the quality of the PATEN. This study uses a descriptive qualitative research method. Triangulation of data or sources technique is used in this study to obtain a data validity. Data collected by field research and interviews with relevant parties. This research was carried out at Temanggung Regency.Results of this study describe the implementation of the Integrated Administrative Service District (PATEN) which can be seen from the substantive requirements, administrative and technical, the type of services provided and the flow of services, financing, human resources, as well as the completeness of infrastructure and facilities. In its implementation found that inhibiting factors internal and external factors. In addition, it was found that several attempts have been made to improve the quality of the PATEN. Recommendations are given, the expected the implementation of the Integrated Administrative Service District (PATEN) in Temanggung Regency work effectively and efficiently

Standardisasi Keselamatan dan Kesehatan Kerja (K3) di Ruang Kerja Praktik Tata Busana pada Kelas XI SMK Negeri 1 Brondong Lamongan

This study aims to describe the standardization of OSH in the fashion practice workspace of SMK Negeri 1 Brondong Lamongan, using a qualitative descriptive method, data collection techniques in the form of observation, questionnaires, interviews and documentation. The research instruments used were observation sheets and student response questionnaires totaling 24 students of class XI. The basis for OSH standardization uses the Undang-Undang Dasar Peraturan Pemerintah Republik Indonesia No.1 Tahun 1970 tentang keselamatan kerja. The results of this study indicate that: (1) Observations on the application of OSH in the practical workspace have not met the standards. (2) The participants' responses to the OSH questionnaire obtained results that 1% of students answered disagree, 23% neutral, 58% agreed, and 18% of students answered strongly agreed, with an overall result of 79 which stated that most students had implemented OSH. (3) the factors that influence the implementation of the completeness of Occapational Safety and Health (OSH) standardization are the budget or finance (economy)