Insights into centriole geometry revealed by cryotomography of doublet and triplet centrioles.

Centrioles are cylindrical assemblies comprised of 9 singlet, doublet, or triplet microtubules, essential for the formation of motile and sensory cilia. While the structure of the cilium is being defined at increasing resolution, centriolar structure remains poorly understood. Here, we used electron cryo-tomography to determine the structure of mammalian (triplet) and Drosophila (doublet) centrioles. Mammalian centrioles have two distinct domains: a 200 nm proximal core region connected by A-C linkers, and a distal domain where the C-tubule is incomplete and a pair of novel linkages stabilize the assembly producing a geometry more closely resembling the ciliary axoneme. Drosophila centrioles resemble the mammalian core, but with their doublet microtubules linked through the A tubules. The commonality of core-region length, and the abrupt transition in mammalian centrioles, suggests a conserved length-setting mechanism. The unexpected linker diversity suggests how unique centriolar architectures arise in different tissues and organisms

CHANGES OF WEIGHT AND IN-KERNEL CONTENT VALUES OF MAIZE HYBRIDS (OCCITAN, COLOMBA, DK-471) AS A RESULT OF DAMAGING BY EUROPEAN CORN BORER (OSTRINIA NUBILALIS HBN)

The research was conducted in the year 2001 on two fields sown with three different maize hybrids and was based on randomly chosen sample plots. The first survey was carried out on the field sown with hybrids COLOMBA and OCCITAN (total area 120 ha), the second survey was performed on 50 ha of hybrid DK-471. The assessment was focused primarily on the difference between the cobs developed by healthy and damaged maize plants with regards to their weight and the content of protein, fat and starch in the maize kernel. The hybrids unambiguously reacted to infestation by the European Corn Borer by cob weight reduction. The decrease of kernel fat content in the case of COLOMBA and that of the starch content in the case of OCCITAN displayed significant dependence on the degree of infestation by the European Corn Borer. The results of the survey verifiably showed that in both cases either metabolism, or incorporation of protein, fat and starch had experienced disorder. A close link has been ascertained between the growth of larvae population and the kernel-cob rate of the examined hybrids. The distribution and number of larvae significantly affected the weight of maize cobs of hybrid DK-471 and the quantity of the basic nutrient components (raw protein, fat and starch) incorporated in the kernel

Possible pair-instability supernovae at solar metallicity from magnetic stellar progenitors

Near-solar metallicity (and low-redshift) Pair-Instability Supernova (PISN) candidates challenge stellar evolution models. Indeed, at such a metallicity, even an initially very massive star generally loses so much mass by stellar winds that it will avoid the electron-positron pair-creation instability. We use recent results showing that a magnetic field at the surface of a massive star can significantly reduce its effective mass-loss rate to compute magnetic models of very massive stars (VMSs) at solar metallicity and explore the possibility that such stars end as PISNe. We implement the quenching of the mass loss produced by a surface dipolar magnetic field into the Geneva stellar evolution code and compute new stellar models with an initial mass of $200\,M_\odot$ at solar metallicity, with and without rotation. It considerably reduces the total amount of mass lost by the star during its life. For the non-rotating model, the total (CO-core) mass of the models is $72.8\,M_\odot$ ($70.1\,M_\odot$) at the onset of the electron-positron pair-creation instability. For the rotating model, we obtain $65.6\,M_\odot$ ($62.4\,M\odot$). In both cases, a significant fraction of the internal mass lies in the region where pair instability occurs in the $\log(T)-\log(\rho)$ plane. The interaction of the reduced mass loss with the magnetic field efficiently brakes the surface of the rotating model, producing a strong shear and hence a very efficient mixing that makes the star evolve nearly homogeneously. The core characteristics of our models indicate that solar metallicity models of magnetic VMSs may evolve to PISNe (and pulsation PISNe).Comment: 4 pages, accepted for publication in A&

Counterions and the bacteriorhodopsin proton pump

AbstractTheoretical and new experimental arguments are given to explain the reversal of photoelectric signals from purple membranes oriented and immobilized in gel due to the presence of TEMED. The continuous current induced by continuous illumination demonstrates a photoelement-like behaviour, the polarity of which is reversed by TEMED. The data render the counterion-collapse mechanism highly questionable

A global profile of replicative polymerase usage

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) α–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polɛ replicates the leading strand, whereas Polδ performs lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Polδ followed by exchange for Polɛ

Changing of flight phenology and ecotype expansion of the European corn borer (Ostrinia nubilalis Hbn.) in Hungary Part

The studies aimed to acquire the widest possible information on the annual flight in Hungary of the European corn borer (ECB), Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). The investigations used biomathematical (Part 1) and graphical (Part 2) evaluation to document changes in the individual population number.The study was conducted in Hungary using ECB moth capture records from the Plant Protection Information System black light trap system (1991–2004). We have drawn conclusions on the appearance of annual flights and the tendency of alterations in flight direction by means of light trap results in four different areas in Hungary. We calculated the flight peak quotients, the individual population numbers of the second flight peak, the distinctions of individual numbers of two flight peaks in this part.As previously published, alterations in flight direction of ECB flights began at different times in Hungary. In the current study, a gradual disappearance of the univoltine ecotype and gradual appearance of the bivoltine ecotype ECB in Hungary is confirmed by the data obtained between 1991–2004. Flight peak quotients and data concerning the second flight peak have confirmed change this process, too: the appearance of a second flight peak in Northwestern Hungary from 1995–1996 (FP = 1.27), the more significant appearance of flights in August in Western Hungary (FP = 1.05) and Northeastern Hungary (FP = 1.45), and a three and four times more individual number of the second flight peak in Southeastern Hungary (FP = 3.44). Flight peak quotients, individual population numbers of the second flight peak, the tendency towards a difference in population number of the two peaks, and size of increase of these values demonstrates the southeastern-northwestern presence of the bivoltine ecotype in Hungary

The effects of surface fossil magnetic fields on massive star evolution. III:The case of τ Sco

$\tau$ Sco, a well-studied magnetic B-type star in the Upper Sco association, has a number of surprising characteristics. It rotates very slowly and shows nitrogen excess. Its surface magnetic field is much more complex than a purely dipolar configuration which is unusual for a magnetic massive star. We employ the CMFGEN radiative transfer code to determine the fundamental parameters and surface CNO and helium abundances. Then, we employ MESA and GENEC stellar evolution models accounting for the effects of surface magnetic fields. To reconcile $\tau$ Sco's properties with single-star models, an increase is necessary in the efficiency of rotational mixing by a factor of 3 to 10 and in the efficiency of magnetic braking by a factor of 10. The spin down could be explained by assuming a magnetic field decay scenario. However, the simultaneous chemical enrichment challenges the single-star scenario. Previous works indeed suggested a stellar merger origin for $\tau$ Sco. However, the merger scenario also faces similar challenges as our magnetic single-star models to explain $\tau$ Sco's simultaneous slow rotation and nitrogen excess. In conclusion, the single-star channel seems less likely and versatile to explain these discrepancies, while the merger scenario and other potential binary-evolution channels still require further assessment as to whether they may self-consistently explain the observables of $\tau$ Sco.Comment: Accepted for publication in MNRAS. A full reproduction package is shared on zenodo in accordance with the Research Data Management plan of the Anton Pannekoek Institute for Astronomy at the University of Amsterdam: 10.5281/zenodo.463340