HPLC analysis of technetium(I)-99m labelled C60(OH)22

Fullerenols, water-soluble polihydroxylated fullerenes, are very important kinds of fullerene derivatives because it is suitable for biological study. In order to get convenient substance for studies (in vivo and in vitro) we investigate possibilities of labeling fullerenol. The HPLC results performed by isocratic HPLC, confirmed that hydrophilic organometallic [99mTc(CO)3(H2O)3]+ precursor allows forming of 99mTc(I) complexes with fullerenol.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

NORMALIZATION OF HEALTH RECORDS IN THE SERBIAN LANGUAGE WITH THE AIM OF SMART HEALTH SERVICES REALIZATION

The development of information technology increases its use in various spheres of human activity, including healthcare. Bundles of data and reports are generated and stored in textual form, such as symptoms, medical history, and doctor’s observations of patients' health. Electronic recording of patient data not only facilitates day-to-day work in hospitals, enables more efficient data management and reduces material costs, but can also be used for further processing and to gain knowledge to improve public health. Publicly available health data would contribute to the development of telemedicine, e-health, epidemic control, and smart healthcare within smart cities. This paper describes the importance of textual data normalization for smart healthcare services. An algorithm for normalizing medical data in Serbian is proposed in order to prepare them for further processing (F1-score=0,816), in this case within the smart health framework. By applying this algorithm, in addition to the normalized medical records, corpora of keywords and stop words, which are specific to the medical domain, are also obtained and can be used to improve the results in the normalization of medical textual data.

Partitioning of quinapril anion between cetyltrimethylammonium bromide micelles and water

The interaction of the anion of quinapril (QUIN), angiotensin converting enzyme (ACE) inhibitor, with cationic surfactant cetyltrimethylammonium bromide (CTAB) was studied as a model system for drug/membrane interactions. From the dependence of differential absorbance at λ=272 nm on CTAB concentration, by using mathematical model that treats the solubilization of QUIN anion as its binding to specific sites in the micelles (Langmuir adsorption isotherm), the binding constant Kb was obtained.Physical chemistry 2006 : 8th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-29 September 200

Surface modification of TiO2 nanoparticles with catechol

Surface modification of nanocrystalline TiO2 particles with catechol was found to result in a red shift of the semiconductor absorption compared to unmodified nanocrystallites. The undercoordinated defect sites at TiO2 surface are the source of novel enhanced and selective reactivity of the nanoparticle toward bidentate ligand binding. Catechol, an enediol ligand, have the optimal geometry for chelating surface Ti atoms, resulting in a five-membered ring coordination complex and restoration of six-coordinated octahedral geometry of surface Ti atoms.Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 24-28 September 200

Gladiatorial games in province of Dalmatia: the role of spectacles in construction of Roman identity

Rad se bavi istraživanjem gladijatorskih spektakala i njihovog značaja u konstruisanju lokalnih identiteta u imperijalnom kontekstu provincije Dalmacije. Gladijatorski spektakli predstavljaju jedan od važnijih aspekata svakodnevnog života u društvima rimskih provincija. Međutim, pored velikog broja radova koji se bave različitim aspektima spektakala, (gladijatorima, organizacijom ili izgradnjom amfiteatara i sl.), malo pažnje je posvećeno ulozi koju igraju prilikom kontakta rimske imperijalne vlasti i lokalnih zajednica. Najveći broj radova, opterećen je tradicionalnim konceptom romanizacije koji podrazumeva binarne pozicije Rimljana i lokalnih zajednica i u kome su Rimljani superiorni u tehnološkom, vojnom, ekonomskom, političkom ili kulturnom smislu. Stoga se i proces promena posmatra jednosmerno, gde lokalne kulture postaju u sve većoj meri „rimske“. Pored ovakve prilično uprošćene pozicije, koncept podrazumeva i uvođenje termina „stepen romanizacije“ koji se obično meri prisustvom i obimom materijalne kulture koja je prethodno obeležena kao „rimska“. U takvom kontekstu spektakli postaju signal već završenog procesa romanizacije u kome nema mesta za lokalna razumevanja, nove rimske kulture. U ovom radu u potpunosti je napušten tradicionalni koncept romanizacije i otvoren je prostor za drugačije perspektive, koje se baziraju, pre svega, na teoriji identiteta. U tom smislu, materijalna kultura se posmatra kao sredstvo za konstrukciju, održavanje i naglašavanje društvenih identiteta. Ciljevi istraživanja su (1) razmatranje materijalne kulture u svetlu konstruisanja lokalnih identiteta, (2) prepoznavanje odnosa različitih individualnih i kolektivnih identiteta, kako bi se što jasnije predstavile prakse čije razumevanje vodi ka objašnjenju provincijalnog koncepta „biti Rimljanin“ u Dalmaciji i (3) pokušaj definisanja odnosa lokalnog provincijalnog društva prema učesnicima gladijatorskih spektakala, njihovog statusa i uloge u društvu rimske Dalmacije. Rezultati istraživanja baziraju se na uzorku koji se sastoji od građevinskih objekata (amfiteatara), epigrafskog i pokretnog arheološkog materijala. Raznovrsnost uzorka omogućila je uvid u različite aspekte organizacije igara, izgradnje objekata u Dalmaciji, popularnosti spektakla među njenim stanovništvom, ali i načina života i statusa učesnika spektakla. Pored osnovnih pitanja postavljenih u radu, tokom diskusije je otvoreno još važnih pitanja. Posebno bi trebalo naglasiti ulogu vojske u organizaciji spektakla. Trebalo bi pomenuti i važnost proučavanja svakodnevnice u razumevanju kulturnih promena. Dok rimski natpisi, svakodnevni predmeti ili arhitektura ne moraju nužno značiti dublje društvene promene (kako tradicionalan pogled na romanizaciju podrazumeva), običaji koji podrazumevaju nove svakodnevne prakse poput odlaska u kupatilo, teatar ili amfiteatar mogu biti indikator takvih procesa. Upravo takve promene na svakodnevnom planu vode do rasprave o širim promenama u okviru zajednica u prošlosti. Bez obzira sa koje strane posmatramo promene, da li podrazumevamo namernu politiku Rima ili spontane procese u sudaru različitih kulturnih ili političkih elemenata, smatram da upravo svakodnevnica može biti ključ za razumevanje zajednica u prošlosti i načina na koji su doživljavali sebe i svet oko sebe.This paper deals with gladiatorial spectacles and their significance in construction of local identities within imperial context of the Roman province of Dalmatia. The spectacles present one important aspect of provincial societies’ everyday life. However, despite great number of papers concerning different aspects of the spectacles, i.e., gladiators’ status, organization of the games or amphitheatre building, little attention has been paid to their role within contexts of contacts between Roman imperial authorities and local communities, which had no previous knowledge of spectacle concept. Most of these papers are burdened with traditional Romanization concept, which implies binary oppositions between the Romans and the local communities, supposing the Romans as technologically, militarily, economically, politically or culturally superior. In such interpretative setting, change is always perceived as unidirectional and the locals are supposed to be trying to become more Roman. Besides this simplified understanding of the identity constructions, the concept also involves the term of ‘Romanization level’ which, supposedly, can be measured by the amount of material culture previously labeled as Roman. In such a context, appearances of the spectacles are perceived as signs of fully accomplished process of Romanization, where one could not find place for local understanding of Roman culture. This paper abandons the traditional concept of Romanization and opens space for different perspectives on identity construction wherein material culture presents an important agency in the processes of construction, maintenance and emphasizing the local social identities. Research goals of this research are: (1) consideration of the material culture as an active participant in the local identities construction; (2) recognizing the importance of individual and collective identities interrelations in order to more accurately present practices of provincial concept of „being Roman“; and (3) defining the relations between the local societies as participants in gladiatorial spectacles and their subsequent emerging status and roles in society of Roman Dalmatia. Results derive from a sample consisting of: architectural structures (amphitheaters), epigraphic and archaeological finds. Varieties of the sample enable some insight into the different aspects of the game organization, architectural structures in Dalmatia, popularity of the spectacles with the population of the province, and the way of life and social status of the participants in the spectacles. Beside these basic questions, more problems that are important arise within discussion. The importance of Roman army in organization of the spectacles, and significance of leisure studies for understanding of the cultural changes are the most prominent ones. While Latin inscriptions, everyday objects or architecture do not necessary imply some deeper social changes, as the traditional Romanization concept implies, new customs including everyday practices and routines (e.g.: visiting baths, theaters or amphitheaters) indicate some social changes. These changes of everyday life enable the discussion of wider social changes in the past. Regardless of our point of view, whether we look on those changes as a result of deliberate Roman policy or spontaneous moves in collision of the different cultural and political elements, leisure and everyday life may be the key for better understanding of the past societies and their perception of one’s position within the local and imperial world

Derivative spectrophotometric determination of partition coefficient of hydrochlorothiazide between cetyltrimethylammonium bromide micelles and water

The interaction of hydrochlorothiazide (HCT), benzothiadiazine diuretic, with cationic surfactant cetyltrimethylammonium bromide (CTAB) was studied as a model system for drug/membrane interactions. From the dependence of first order derivative amplitude 1 D250.1 on CTAB concentration, by using mathematical models based on the partition of the drug between micellar and aqueous pseudo-phase, CTAB/water partition coefficient Kp was calculated.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

Viruses and apoptosis

Apoptoza je mehanizam kojim se neželjene stanice odstranjuju iz organizma, a poremećaji u regulaciji apoptoze čimbenici su u mnoštvu patoloških stanja. Obzirom na važnu ulogu apoptoze u djelovanju imunološkog sustava, virusi su razvili mnogobrojne mehanizme kojima ometaju njezin proces i time osiguravaju uspješno razmnožavanje i širenje. Sprečavanjem ili odgodom apoptoze može se produljiti raspoloživi vremenski period za proizvodnju novih viriona prije nego putovi biosinteze stanice domaćina, kojima se virus koristi, budu razoreni. S druge strane, određeni genski proizvodi virusa djeluju kao induktori apoptoze, vjerojatno s ciljem otpuštanja i širenja viriona iz inficirane stanice domaćina bez poticanja odgovarajućeg imunološkog odgovora. Ovim smo člankom željeli sažeti glavne mehanizme stanične apoptoze i pružiti primjere na koji način virusi i njihovi genski produkti mogu utjecati na taj proces. Napredak molekularne biologije otvorio je epohu u kojoj bi se virusni geni mogli iskoristiti za proizvodnju čitavog niza terapeutika.Apoptosis is an active process of cell death that doesn\u27t initiate immune system response. Manipulation of the signalling cascades regulating apoptosis can result in a wide variety of human diseases. Viruses have the ability to affect the apoptotic process within the host cell. They can benefit either from the promotion or the inhibition of apoptosis. The benefits of virus avoiding the apoptotic process are obvious but the onset of apoptosis can also be advantageous. It enables the dissemination of virus without initiating a concomitant inflammatory host response. The purpose of this article was to give an overview of the mechanisms of apoptosis and examples for some of the many ways in which viruses and their gene products can influence this process. The progress of molecular biology has opened an era in which viral genes could be used for the production of different therapeutics

Double Tooth

The form of primary and permanent teeth can differ morphologically from that which is considered normal, completely or in some parts. The changes in tooth form can be hereditary or caused by some disease or trauma. Fusion is a union of one or more teeth during development. Gemination means that two separate morphological units were created by division of the tooth germ. The intention of this study was to state the prevalence of double teeth (fusion and gemination) among the persons tested, as to gender, distribution in the maxilla or mandible, and whether the anomaly occurred bilaterally or unilaterally. The results of this investigation have shown that in a total of examined 3,517 plaster models, a prevalence of double teeth was 0.2%. 57.2% of them were fusioned and 42.9% geminated

Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?

Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted. [Projekat Ministarstva nauke Republike Srbije, br. 41008 and br. 173048