Biochemical and molecular characterization of olive β-glucosidase in seven olive varieties during the ripening process: the role of β-glucosidase in determining the phenolic content of virgin olive oil.

Virgin olive oil (VOO) is one of the essential components of the Mediterranean diet, which includes a series of cultural habits, especially alimentary, shared to a greater or lesser extent, by all countries lapped by Mediterranean Sea. VOO is enriched with bioactive compounds which are related to its unique organoleptic characteristics, and also to its antioxidant properties, which have been associated to with the reduction of risk to suffer cardiovascular diseases and to a protective effect against cancer. The most important bioactive components in VOO are phenolics compounds [2].The phenolic composition of VOO is closely related to the content of phenolic glycosides initially present in the olive tissue. In parallel, some studies indicate that there is a positive correlation between the activity levels of β-glucosidase in the olive fruit and the final content of phenolic compounds in VOO. This enzyme plays a key role hydrolyzing phenolic profile of VOO. In consequence, its biochemical and molecular characterization is of great interest from a biotechnological point of view [1, 2].In this study, seven olive varieties (Abou-Kanani, Dokkar, Klon-14, Menya, Picual, Piñonera y Shengeh) with different phenolic contents have been selected in the World Olive Germplasm Bank. The phenolic profiles of fruits harvested at different ripening stages and their oils have been analyzed by HPLC and at the same time β-glucosidase activity has been measured in olive fruits at different ripening stages. The methods for β-glucosidase extraction and activity assessment have been optimized. Spectrophotometric activity assays have been carried out with the synthetic substrate p-nitrophenyl galactoside (p-NPG) using a calculated molar extinction coefficient (ε=552,8 M-1cm-1) for the p-nitrophenyl liberated in the reaction medium. The results obtained show that olive varieties with the highest content of phenolic glycosides in the fruit such as Piñonera, Dokkar or Menya, also have the largest β-glucosidase activity levels, which usually decrease ripening process. These results will be completed with the pertinent studies of gene expression [3]

Informe GUESSS Universidad de Jaén 2021. El espíritu emprendedor de los estudiantes universitarios

La Universidad de Jaén apuesta con decisión por el emprendimiento y prueba de ello es la participación activa en el proyecto GUESSS (Global University Entrepreneurial Spirit Student´s Survey) desde el año 2018, en el que este proyecto se integra dentro de las actividades realizadas por el Observatorio de Emprendimiento de España, liderado por el profesor José Ruiz Navarro y su equipo en la Universidad de Cádiz. El proyecto GUESSS se realiza con carácter bienal y la Universidad de Jaén ha participado en el mismo en varias convocatorias consecutivas. No obstante, éste es el primer informe del espíritu emprendedor del estudiantado de la UJA. Esto ha sido posible por la reciente creación de la Cátedra Universitaria de Emprendimiento Fulgencio Meseguer, cuyos miembros han procesado la información de la encuesta y redactado el informe GUESSS Jaén 2021. El informe actual analiza los datos recopilados en el periodo comprendido entre los meses de marzo y mayo de 2021 a través de un cuestionario que fue distribuido a estudiantes de grado y posgrado de todas las titulaciones. La difusión de la encuesta se hizo con el apoyo del Secretariado de Empleabilidad y Emprendimiento de la UJA. Después de un envío inicial en marzo de 2021 y de un recordatorio de seguimiento realizado tres semanas después, se recogieron un total de 810 respuestas válidas, lo que supone una tasa de respuesta del 5,9%. El objetivo de esta publicación es tratar de aportar información que pueda contribuir a la compresión de los factores que influyen en las intenciones emprendedoras de los estudiantes universitarios. Los resultados que se derivan del estudio permiten aproximar el emprendimiento universitario, de forma que los agentes relacionados, tales como universidades, gobiernos y empresas, puedan disponer de herramientas adicionales que permitan la adopción de decisiones que contribuyan a dinamizar este aspecto en beneficio de nuestra sociedad. Más concretamente, las conclusiones de este informe GUESSS en la Universidad de Jaén presentan beneficios para diferentes grupos de interés. En primer lugar, la identificación de la posición relativa de la Universidad de Jaén permite evaluar la intención de crear un negocio por parte de los estudiantes de una determinada zona geográfica y valorar los indicadores obtenidos en el contexto nacional (o internacional). Una correcta interpretación de los resultados permitirá evaluar la situación y ofrecer recomendaciones que faciliten el diseño de políticas públicas en materia de emprendimiento. Adicionalmente, este tipo de trabajos permite poner el foco de atención en el espíritu emprendedor y ayuda a sensibilizar a la sociedad sobre la relevancia de la iniciativa empresarial. En segundo lugar, para las universidades es una herramienta que les permite evaluar si sus programas de formación y el contexto de la propia universidad contribuyen a la formación de intenciones emprendedoras. Por último, la participación en las encuestas ofrece a los estudiantes la posibilidad de recapacitar sobre sus objetivos profesionales y les ayuda a plantearse, con una orientación estratégica, su carrera profesional. En este informe también se aportan pruebas del emprendimiento entre nuestros estudiantes, como lo demuestran los 8 casos que se incluyen y que recogen muestras de emprendimiento femenino, rural, innovador y multidisciplinar. Estamos seguros de que servirán de ejemplo para los estudiantes de nuestra Universidad y para que muchos de ellos elijan el emprendimiento como una opción profesional. No queremos terminar sin agradecer el esfuerzo de todos aquellos que hacen posible el desarrollo del proyecto: a José Ruiz Navarro, coordinador GUESSS a nivel nacional, así como a todos los miembros de su equipo por el trabajo y el apoyo recibido a lo largo de todo este proceso tanto de la administración de la encuesta, análisis de datos, creación de informes, etc., a la Fundación Fulgencio Meseguer por su patrocinio, al Secretariado de Empleabilidad y Emprendimiento de la UJA, a los compañeros del equipo GUESSS Jaén y, finalmente, a todos los encuestados que han hecho posible que este trabajo sirva de referente y guía en el ámbito del emprendimiento.Cátedra Universitaria de Emprendimiento Fulgencio Meseguer. Fundación Fulgencio Mesegue

Revealing nature of GRB 210205A, ZTF21aaeyldq (AT2021any), and follow-up observations with the 4K×\times4K CCD Imager+3.6m DOT

Optical follow-up observations of optical afterglows of gamma-ray bursts are crucial to probe the geometry of outflows, emission mechanisms, energetics, and burst environments. We performed the follow-up observations of GRB 210205A and ZTF21aaeyldq (AT2021any) using the 3.6m Devasthal Optical Telescope (DOT) around one day after the burst to deeper limits due to the longitudinal advantage of the place. This paper presents our analysis of the two objects using data from other collaborative facilities, i.e., 2.2m Calar Alto Astronomical Observatory (CAHA) and other archival data. Our analysis suggests that GRB 210205A is a potential dark burst once compared with the X-ray afterglow data. Also, comparing results with other known and well-studied dark GRBs samples indicate that the reason for the optical darkness of GRB 210205A could either be intrinsic faintness or a high redshift event. Based on our analysis, we also found that ZTF21aaeyldq is the third known orphan afterglow with a measured redshift except for ZTF20aajnksq (AT2020blt) and ZTF19abvizsw (AT2019pim). The multiwavelength afterglow modelling of ZTF21aaeyldq using the afterglowpy package demands a forward shock model for an ISM-like ambient medium with a rather wider jet opening angle. We determine circumburst density of $n_{0}$ = 0.87 cm$^{-3}$, kinetic energy $E_{k}$ = 3.80 $\times 10^{52}$ erg and the afterglow modelling also indicates that ZTF21aaeyldq is observed on-axis ($\theta_{obs} < \theta_{core}$) and a gamma-ray counterpart was missed by GRBs satellites. Our results emphasize that the 3.6m DOT has a unique capability for deep follow-up observations of similar and other new transients for deeper observations as a part of time-domain astronomy in the future.Comment: Accepted for Special Issue of Journal of Astrophysics and Astronomy, 2022, Astrophysical jets and observational facilities: National perspective, 05 -09 April 2021, ARIES Nainita

Increasing crop rotational diversity can enhance cereal yields

9 Pág.Diversifying agriculture by rotating a greater number of crop species in sequence is a promising practice to reduce negative impacts of crop production on the environment and maintain yields. However, it is unclear to what extent cereal yields change with crop rotation diversity and external nitrogen fertilization level over time, and which functional groups of crops provide the most yield benefit. Here, using grain yield data of small grain cereals and maize from 32 long-term (10–63 years) experiments across Europe and North America, we show that crop rotational diversity, measured as crop species diversity and functional richness, enhanced grain yields. This yield benefit increased over time. Only the yields of winter-sown small grain cereals showed a decline at the highest level of species diversity. Diversification was beneficial to all cereals with a low external nitrogen input, particularly maize, enabling a lower dependence on nitrogen fertilisers and ultimately reducing greenhouse gas emissions and nitrogen pollution. The results suggest that increasing crop functional richness rather than species diversity can be a strategy for supporting grain yields across many environments.G.V., R.B. and S.H. acknowledge FORMAS grants 2018-02872 and 2018-02321. TMB acknowledges USDA AFRI grant 2017-67013-26254. LTEs managed by SRUC were supported by the Scottish Government RESAS Strategic Research Programme under project D3-, Healthy Soils for a Green Recovery. Swedish LTEs were funded by the Swedish University of Agricultural Sciences (SLU). We thank the Lawes Agricultural Trust and Rothamsted Research for data from the e-RA database. The Rothamsted Long-term Experiments National Capability (LTE-NC) was supported by the UK BBSRC (Biotechnology and Biological Sciences Research Council, BBS/E/C/000J0300) and the Lawes Agricultural Trust. The Woodslee site was supported by the Agro-Ecosystem Resilience Program (Agriculture & Agri-Food Canada) and field management provided by field crews over 6 decades is appreciated. La Canaleja LTE (Spain) was supported by RTA2017-00006-C03-01 project (Ministry of Science and Innovation. El Encín LTEs were supported by Spanish Ministry of Economy and Competitiveness funds (projects AGL2002-04186-C03-01.03, AGL2007-65698-C03-01.03, AGL2012-39929-C03-01 of which L. Navarrete was the P.I). R.A., A.G.D. and E.H.P. are also grateful to all members of the Weed Science Group from El Encín Experimental Station for their technical assistance in managing the experiments. The Brody/Poznan University of Life Sciences long-term experiments were funded by the Polish Ministry of Education and Science. We acknowledge the E-Obs dataset from the EU-FP6 project UERRA (http://www.uerra.eu) and the Copernicus Climate Change Service, and the data providers in the ECA&D project (https://www.ecad.eu/).Peer reviewe

Crop rotational diversity can mitigate climate-induced grain yield losses

Diversified crop rotations have been suggested to reduce grain yield losses from the adverse climatic conditions increasingly common under climate change. Nevertheless, the potential for climate change adaptation of different crop rotational diversity (CRD) remains undetermined. We quantified how climatic conditions affect small grain and maize yields under different CRDs in 32 long-term (10-63 years) field experiments across Europe and North America. Species-diverse and functionally rich rotations more than compensated yield losses from anomalous warm conditions, long and warm dry spells, as well as from anomalous wet (for small grains) or dry (for maize) conditions. Adding a single functional group or crop species to monocultures counteracted yield losses from substantial changes in climatic conditions. The benefits of a further increase in CRD are comparable with those of improved climatic conditions. For instance, the maize yield benefits of adding three crop species to monocultures under detrimental climatic conditions exceeded the average yield of monocultures by up to 553 kg/ha under non-detrimental climatic conditions. Increased crop functional richness improved yields under high temperature, irrespective of precipitation. Conversely, yield benefits peaked at between two and four crop species in the rotation, depending on climatic conditions and crop, and declined at higher species diversity. Thus, crop species diversity could be adjusted to maximize yield benefits. Diversifying rotations with functionally distinct crops is an adaptation of cropping systems to global warming and changes in precipitation.</p

The CARMENES search for exoplanets around M dwarfs High-resolution optical and near-infrared spectroscopy of 324 survey stars

The CARMENES radial velocity (RV) survey is observing 324 M dwarfs to search for any orbiting planets. In this paper, we present the survey sample by publishing one CARMENES spectrum for each M dwarf. These spectra cover the wavelength range 520–1710 nm at a resolution of at least R >80 000, and we measure its RV, Hα emission, and projected rotation velocity. We present an atlas of high-resolution M-dwarf spectra and compare the spectra to atmospheric models. To quantify the RV precision that can be achieved in low-mass stars over the CARMENES wavelength range, we analyze our empirical information on the RV precision from more than 6500 observations. We compare our high-resolution M-dwarf spectra to atmospheric models where we determine the spectroscopic RV information content, Q, and signal-to-noise ratio. We find that for all M-type dwarfs, the highest RV precision can be reached in the wavelength range 700–900 nm. Observations at longer wavelengths are equally precise only at the very latest spectral types (M8 and M9). We demonstrate that in this spectroscopic range, the large amount of absorption features compensates for the intrinsic faintness of an M7 star. To reach an RV precision of 1 m s−1 in very low mass M dwarfs at longer wavelengths likely requires the use of a 10 m class telescope. For spectral types M6 and earlier, the combination of a red visual and a near-infrared spectrograph is ideal to search for low-mass planets and to distinguish between planets and stellar variability. At a 4 m class telescope, an instrument like CARMENES has the potential to push the RV precision well below the typical jitter level of 3–4 m s−1

The CARMENES search for exoplanets around M dwarfs. Two temperate Earth-mass planet candidates around Teegarden’s Star

Context.Teegarden’s Star is the brightest and one of the nearest ultra-cool dwarfs in the solar neighbourhood. For its late spectral type (M7.0 V),the star shows relatively little activity and is a prime target for near-infrared radial velocity surveys such as CARMENES.Aims.As part of the CARMENES search for exoplanets around M dwarfs, we obtained more than 200 radial-velocity measurements of Teegarden’sStar and analysed them for planetary signals.Methods.We find periodic variability in the radial velocities of Teegarden’s Star. We also studied photometric measurements to rule out stellarbrightness variations mimicking planetary signals.Results.We find evidence for two planet candidates, each with 1.1M⊕minimum mass, orbiting at periods of 4.91 and 11.4 d, respectively. Noevidence for planetary transits could be found in archival and follow-up photometry. Small photometric variability is suggestive of slow rotationand old age.Conclusions.The two planets are among the lowest-mass planets discovered so far, and they are the first Earth-mass planets around an ultra-cooldwarf for which the masses have been determined using radial velocities.We thank the referee Rodrigo Díaz for a careful review andhelpful comments. M.Z. acknowledges support from the Deutsche Forschungs-gemeinschaft under DFG RE 1664/12-1 and Research Unit FOR2544 “BluePlanets around Red Stars”, project no. RE 1664/14-1. CARMENES isan instrument for the Centro Astronómico Hispano-Alemán de Calar Alto(CAHA, Almería, Spain). CARMENES is funded by the German Max-Planck-Gesellschaft (MPG), the Spanish Consejo Superior de InvestigacionesCientíficas (CSIC), the European Union through FEDER/ERF FICTS-2011-02 funds, and the members of the CARMENES Consortium (Max-Planck-Institut für Astronomie, Instituto de Astrofísica de Andalucía, LandessternwarteKönigstuhl, Institut de Ciències de l’Espai, Institut für Astrophysik Göttingen,Universidad Complutense de Madrid, Thüringer Landessternwarte Tautenburg,Instituto de Astrofísica de Canarias, Hamburger Sternwarte, Centro de Astro-biología and Centro Astronómico Hispano-Alemán), with additional contribu-tions by the Spanish Ministry of Economy, the German Science Foundationthrough the Major Research Instrumentation Programme and DFG ResearchUnit FOR2544 “Blue Planets around Red Stars”, the Klaus Tschira Stiftung, thestates of Baden-Württemberg and Niedersachsen, and by the Junta de Andalucía.Based on data from the CARMENES data archive at CAB (INTA-CSIC). Thisarticle is based on observations made with the MuSCAT2 instrument, devel-oped by ABC, at Telescopio Carlos Sánchez operated on the island of Tener-ife by the IAC in the Spanish Observatorio del Teide. Data were partly col-lected with the 150-cm and 90-cm telescopes at the Sierra Nevada Observa-tory (SNO) operated by the Instituto de Astrofísica de Andalucía (IAA-CSIC).Data were partly obtained with the MONET/South telescope of the MOnitoringNEtwork of Telescopes, funded by the Alfried Krupp von Bohlen und HalbachFoundation, Essen, and operated by the Georg-August-Universität Göttingen,the McDonald Observatory of the University of Texas at Austin, and the SouthAfrican Astronomical Observatory. We acknowledge financial support from theSpanish Agencia Estatal de Investigación of the Ministerio de Ciencia, Inno-vación y Universidades and the European FEDER/ERF funds through projectsAYA2015-69350-C3-2-P, AYA2016-79425-C3-1/2/3-P, AYA2018-84089, BES-2017-080769, BES-2017-082610, ESP2015-65712-C5-5-R, ESP2016-80435-C2-1/2-R, ESP2017-87143-R, ESP2017-87676-2-2, ESP2017-87676-C5-1/2/5-R, FPU15/01476, RYC-2012-09913, the Centre of Excellence ”Severo Ochoa”and ”María de Maeztu” awards to the Instituto de Astrofísica de Canarias (SEV-2015-0548), Instituto de Astrofísica de Andalucía (SEV-2017-0709), and Cen-tro de Astrobiología (MDM-2017-0737), the Generalitat de Catalunya throughCERCA programme”, the Deutsches Zentrum für Luft- und Raumfahrt throughgrants 50OW0204 and 50OO1501, the European Research Council through grant694513, the Italian Ministero dell’instruzione, dell’università de della ricerca andUniversità degli Studi di Roma Tor Vergata through FFABR 2017 and “Mis-sion: Sustainability 2016”, the UK Science and Technology Facilities Council through grant ST/P000592/1, the Israel Science Foundation through grant848/16, the Chilean CONICYT-FONDECYT through grant 3180405, the Mexi-can CONACYT through grant CVU 448248, the JSPS KAKENHI through grantsJP18H01265 and 18H05439, and the JST PRESTO through grant JPMJPR1775

The CARMENES search for exoplanets around M dwarfs HD147379 b: A nearby Neptune in the temperate zone of an early-M dwarf

We report on the first star discovered to host a planet detected by radial velocity (RV) observations obtained within the CARMENES survey for exoplanets around M dwarfs. HD 147379 (V = 8.9 mag, M = 0.58 ± 0.08 M⊙), a bright M0.0 V star at a distance of 10.7 pc, is found to undergo periodic RV variations with a semi-amplitude of K = 5.1 ± 0.4 m s−1 and a period of P = 86.54 ± 0.06 d. The RV signal is found in our CARMENES data, which were taken between 2016 and 2017, and is supported by HIRES/Keck observations that were obtained since 2000. The RV variations are interpreted as resulting from a planet of minimum mass mP sin i = 25 ± 2 M⊕, 1.5 times the mass of Neptune, with an orbital semi-major axis a = 0.32 au and low eccentricity (e < 0.13). HD 147379 b is orbiting inside the temperate zone around the star, where water could exist in liquid form. The RV time-series and various spectroscopic indicators show additional hints of variations at an approximate period of 21.1 d (and its first harmonic), which we attribute to the rotation period of the star.FEDER/ERF FICTS-2011-02 fundsMajor Research Instrumentation Programme and DFG Research Unit FOR2544 “Blue Planets around Red StarsEuropean Research Council (ERC-279347), Deutsche Forschungsgemeinschaft (RE 1664/12-1, RE 2694/4-1), Bundesministerium für Bildung und Forschung (BMBF-05A14MG3, BMBF-05A17MG3), Spanish Ministry of Economy and Competitiveness (MINECO, grants AYA2015-68012-C2-2-P, AYA2016-79425-C3-1,2,3-P, AYA2015-69350-C3-2-P, AYA2014-54348-C03- 01, AYA2014-56359-P, AYA2014-54348-C3-2-R, AYA2016-79425-C3-3-P and 2013 Ramòn y Cajal program RYC-2013-14875), Fondo Europeo de Desarrollo Regional (FEDER, grant ESP2016-80435-C2-1-R, ESP2015-65712-C5- 5-R), Generalitat de Catalunya/CERCA programme, Spanish Ministerio de Educación, Cultura y Deporte, programa de Formación de Profesorado Universitario (grant FPU15/01476), Deutsches Zentrum für Luft- und Raumfahrt (grants 50OW0204 and 50OO1501), Office of Naval Research Global (award no. N62909-15-1-2011), Mexican CONACyT grant CB-2012-183007