Isolation of Local Lipolytic Isolate from Domestic Compost

Screening of lipolytic bacteria from domestic compost resulting an isolate namely AL17. Morphological analysis shows that the isolates were rod shape and belong to negative gram bacteria. The 16S rRNAs genes of the bacteria have been sequenced, and phylogenetic analysis showed that the isolates were close to genus Pseudoxanthomonas. The enzyme production was synchronized with bacterial growth and reached a maximum level during the late-stationary phase. The optimum pH and temperature of enzyme activity were at pH 9.0 and 60°C.The isolate also showed alcohol tolerance in medium containing 3% and 5% methanol. The ability of bacterial cells to tolerate methanol is an important cell characteristic that determines their use as a biocatalyst in transesterification and other industrial process

Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java

Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample. The characterization of the genes showed that the genes are high homology to the DNA polymerase B of Crenarhaea phyla. Phylogenetic analysis of the amino acid sequences showed that the enzymes are grouped in a new branch from the other Crenarchaea’s DNA Polymerase B. 3D structure analysis of the enzymes show that the structures are closed to the structure of DNA Polymerase B1 from Sulfolobus solfataricus. The nine structures of the enzymes could be grouped into four different structures

Penerapan Model GARCH dalam Peramalan Volatilitas di Bursa Efek Indonesia

Tujuan penelitian – Resiko dan volatilitas adalah dua hal yang berkaitan terutama dalam penelitian mengenai pasar modal. Pergerakan saham maupun indeks yang dipengaruhi banyak faktor membuat volatilitas umum terjadi dan hal ini tentu mempengaruhi penilaian resiko. Penelitian ini mencoba melihat penggunaan model GARCH dalam menilai volatilitas di Bursa Efek Indonesia. Desain/Metodologi/Pendekatan – Penelitian ini bersifat deskriptif. Kami mennganalisa volatilitas di Bursa Efek Indonesia melalui return dari Indeks Harga Saham Gabungan (IHSG). Metode yang digunakan adalah model simetris GARCH dan asimetris TARCH dan EGARCH. Temuan – Hasil penelitian menunjukan bahwa pasar modal Indonesia memiliki gejala volatility clustering.. Pasar modal Indonesia lebih sensitif terhadap berita negatif daripada positif. Model GARCH yang dapat dilakukan adalah GARCH (1,1), TARCH (1,1) dan EGARCH (1,1) dengan model EGARCH(1,1) memiliki hasil ramalan yang sedikit lebih baik dari kedua model lainnya. Keterbatasan penelitian – Penelitian ini hanya menyasar pada volatilitas IHSG dan penerapan model GARCH untuk analisa dan peramalan. Penelitian selanjutnya dapat mengkaitkan hasil analisa dengan situasi ekonomi nasional maupun global. Originality/value – Penelitian ini menitikberatkan pada analisa univariat dengan memanfaatkan model ekonometrik GARCH

Association of hemochromatosis with infectious diseases: expanding spectrum

SummaryWithholding iron from potential pathogens is a host defense strategy. There is evidence that iron overload per se compromises the ability of phagocytes to kill microorganisms. Several hypotheses exist to explain the association of hemochromatosis with infection. A combination of mechanisms likely contributes to the increase in susceptibility to infection in these patients. A review of the current literature delineating various pathogens to which patients with hemochromatosis are potentially susceptible, and recent advances in the understanding of the association of hemochromatosis with infection, are discussed

Effect of Clark’s twin-block appliance (CTB) and non-extraction fixed mechano-therapy on the pharyngeal dimensions of growing children

Abstract INTRODUCTION: Narrow airway dimensions due to mandibular deficiency can predispose an individual to severe respiratory distress. Hence, treatment with mandibular advancement devices at an early age might help improving the pharyngeal passage and reduce the risk of respiratory difficulties. Therefore, the aim of the current study was to evaluate the mean changes in the pharyngeal dimensions of children with mandibular deficiency treated with Clark\u27s twin-block appliance (CTB) followed by fixed orthodontic treatment. METHODS: Orthodontic records of 42 children with mandibular deficiency were selected. Records comprised three lateral cephalograms taken at the start of CTB treatment, after CTB removal and at the end of fixed appliance treatment, and were compared with 32 controls from the Bolton-Brush study. Friedman test was used to compare pre-treatment, mid-treatment and post-treatment pharyngeal dimensions. Wilcoxon signed rank test was used to compare the airway between pre-treatment and post follow-up controls. Mann-Whitney U test was applied to compare the mean changes in pharyngeal dimensions between treatment group and controls from T2 to T0. Post-hoc Dunnet T3 test was used for multiple comparisons of treatment outcomes after CTB and fixed appliances, taking a p-value of ≤ 0.05 as statistically significant. RESULTS: Superior pharyngeal space (p \u3c 0.001) and upper airway thickness (p = 0.035) were significantly increased after CTB, and the change in superior pharyngeal space remained stable after fixed mechano-therapy. CONCLUSION: CTB can have a positive effect in improving pharyngeal space and the resultant increase in airway remains stable on an average of two and a half years

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nuclear origin in the progeny of a Volkamer lemon #Citrus volkameriana# Ten. & Pasq.) tree. The genetic variability within two species [#Citrus sinensis# (L.) Osbeck (sweet oranges) and #Citrus reticulata# Blanco and relatives (mandarins)] was evaluated. DNA amplified figerprinting with single primers was the more successful technique for discriminating between nucellular and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of #C. sinensis#, all 10 #C. reticulata# cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related. (Résumé d'auteur

Short sleep duration, complaints of vital exhaustion and perceived stress are prevalent among pregnant women with mood and anxiety disorders

Background: Psychiatric disorders have been associated with sleep disorders in men and non-pregnant women, but little is known about sleep complaints and disorders among pregnant women with psychiatric disorders. Methods: A cohort of 1,332 women was interviewed during early pregnancy. We ascertained psychiatric diagnosis status and collect information about sleep duration, daytime sleepiness, vital exhaustion and perceived stress. Logistic regression procedures were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs). Results: Approximately 5.1% of the cohort (n=68) reported having a physician-diagnosis of mood or anxiety disorder before interview. Compared with women without a psychiatric diagnosis, the multivariable-adjusted OR (95% CI) for short sleep duration in early pregnancy (≤6 hours) were 1.95 (1.03-3.69). The corresponding OR (95%CI) for long sleep duration (≥9 hours) during early pregnancy was 1.13 (0.63-2.03). Women with psychiatric disorders had an increased risk of vital exhaustion (OR=2.41; 95%CI 1.46-4.00) and elevated perceived stress (OR=3.33; 95%CI 1.89-5.88). Observed associations were more pronounced among overweight/obese women. Conclusions: Women with a psychiatric disorder were more likely to report short sleep durations, vital exhaustion and elevated perceived stress. Prospective studies are needed to more thoroughly explore factors that mediate the apparent mood/anxiety-sleep comorbidity among pregnant women

Reliability and validity of maxillary and sphenoid Sinus morphological variations in the assessment of skeletal maturity

BACKGROUND: The present study aimed at assessing the relationship between growth changes in maxillary (MS) and sphenoid sinus (SS) and cervical vertebral maturation (CVM) and to evaluate their reliability and validity in assessing the skeletal maturity of an individual. METHODS: A crosssectional study was conducted on the pretreatment lateral cephalograms of 224 patients (males=116, females=108) aged 8-17 years. MS and SS heights, widths and indices were evaluated. The subjects were classified according to six stages based on CVM using Baccetti\u27s method. Kruskal-Wallis test was applied to compare MS and SS measurements at different cervical stages for each gender. Kappa statistics, positive predictive value, negative predictive value, sensitivity and specificity were calculated to test the diagnostic accuracy of MS and SS indices. RESULTS: The MS and SS indices varied significantly (p\u3c0.001) at different cervical stages for both gender. Kappa statistics showed significant agreement using MS (p\u3c0.001) and SS indices (p\u3c0.05). The diagnostic performance of MS index (Sensitivity ≥71%) was found to be better than SS index (Sensitivity ≥65%).. CONCLUSIONS: The MS height, width and index in genders and SS height, width and index in males and only SS width and index in females were significantly associated with the CVM stages. The validity of MS and SS indices were comparable for females; whereas, the MS index offers significant advantage over SS index for the assessment of growth status of males

Organizational health and quality of life: survey among ambulance nurses in prehospital emergency care

Background: The workplace plays a central role in causing stress and different kinds of syndromes and diseases. More generally, organizational procedures and practices could have an impact on nurses’ quality of life. Although several studies have investigated this link, none of them considered nurses working in prehospital emergency care. Objectives: To investigate the role of organizational health factors that affect the quality of life and psychosomatic complaints of ambulance nurses.Method: Our sample included 411 ambulance nurses. Workers were administered two questionnaires to assess organizational health and quality of life. Descriptive and correlational analyses were used to test our assumptions. Conclusion: Several organizational health dimensions provided an explanation for the complaints reported by nurses working in prehospital emergency care in terms of quality of life and psychosomatic disorders.The results allowed identification of possible interventions focusing on specific duties and organizational aspects that would improve the quality of nurses’ health

STRATEGI KOMUNIKASI INTERPERSONAL ORANG TUA TERHADAP ANAK REMAJA DALAM MENGHADAPI PERGAULAN BEBAS DI NEGERI TULEHU KABUPATEN MALUKU TENGAH

Penelitian ini bertujuan untuk Memahami strategi komunikasi interpersonal yang dibangun orangtua terhadap remaja dalam menghadapi pergaulan bebas dikabupaten Maluku Tengah, Mengetahui dan menjelaskan faktor-faktor pendukung dan penghambat strategi komunikasi interpersonal orang tua terhadap anak remaja dalam  menghdapi pergaulan bebas dikabupaten Maluku Tengah. penelitian ini menggunakan pendekatan kulaitatif dengan analisis deskriptif. Data diperoleh melalui  pengamatan, observasi dan wawancara mendalam terhadap 5 informan dan analisis menggunakan Miles dan Huberman. Hasil penelitian menunjukan bahwa yang diperoleh menjelaskan bahwa sebagian orang tua ada yang berhasil  mencegah anak mereka untuk tidak terlibat atau terjerumus ke dalam pergaulan bebas dengan memberikan didikan, bimbingan, nasehat, arahan dan ada pula yang tidak berhasil yang mencegah anak remaja mereka terlibat atau terjerumus ke dalam pergaulan bebas meskipun telah berusaha mencegah dan tak perna berhenti dengan memberikan anak remaja mereka didikan, nasehat, bimbingan dan arahan disebabkan karena lingkungan yang dipengaruhi oleh pergaulan bebas dan media online yang mana lebih dominan berpengaruh atau mempengaruhi anak remaja terhadap pembentukan karakter, sikap dan perilaku anak remaja yang berada di Negeri Tulehu Kabupaten Maluku Tengah