Sexual differentiation of microglia

Sex plays a role in the incidence and outcome of neurological illnesses, also influencing the response to treatments. Despite sexual differentiation of the brain has been extensively investigated, the study of sex differences in microglia, the brain's resident immune cells, has been largely neglected until recently. To fulfill this gap, our laboratory developed several tools, including cellular and animal models, which bolstered in-depth studies on sexual differentiation of microglia and its impact on brain physiology, as well as on the onset and progression of neurological disorders. Here, we summarize the current status of knowledge on the sex-dependent function of microglia, and report recent evidence linking these cells to the sexual bias in the susceptibility to neurological brain diseases

The SXI telescope on board EXIST: scientific performances

The SXI telescope is one of the three instruments on board EXIST, a multiwavelength observatory in charge of performing a global survey of the sky in hard X-rays searching for Supermassive Black Holes. One of the primary objectives of EXIST is also to study with unprecedented sensitivity the most unknown high energy sources in the Universe, like high redshift GRBs, which will be pointed promptly by the Spacecraft by autonomous trigger based on hard X-ray localization on board. The recent addition of a soft X-ray telescope to the EXIST payload complement, with an effective area of ~950 cm2 in the energy band 0.2-3 keV and extended response up to 10 keV will allow to make broadband studies from 0.1 to 600 keV. In particular, investigations of the spectra components and states of AGNs and monitoring of variability of sources, study of the prompt and afterglow emission of GRBs since the early phases, which will help to constrain the emission models and finally, help the identification of sources in the EXIST hard X-ray survey and the characterization of the transient events detected. SXI will also perform surveys: a scanning survey with sky coverage of about 2pi and limiting flux of 5x10^{-14}cgs plus other serendipitous. We give an overview of the SXI scientific performance and also describe the status of its design emphasizing how it has been derived by the scientific requirements.Comment: 9 pages, 6 figures, to be published in Proc. of SPIE, vol 7435-11, 200

Anterior cruciate ligament injury mechanisms through a neurocognition lens: implications for injury screening

Athletes in team sports have to quickly visually perceive actions of opponents and teammates while executing their own movements. These continuous actions are performed under time pressure and may contribute to a non-contact ACL injury. However, ACL injury screening and prevention programmes are primarily based on standardised movements in a predictable environment. The sports environment provides much greater cognitive demand because athletes must attend their attention to numerous external stimuli and inhibit impulsive actions. Any deficit or delay in attentional processing may contribute to an inability to correct potential errors in complex coordination, resulting in knee positions that increase the ACL injury risk. In this viewpoint, we advocate that ACL injury screening should include the sports specific neurocognitive demands

Systematic Video Analysis of Anterior Cruciate Ligament Injuries in Professional Male Rugby Players: Pattern, Injury Mechanism, and Biomechanics in 57 Consecutive Cases

Background: Anterior cruciate ligament (ACL) injuries represent a significant burden to rugby players. Improving our understanding of the patterns and biomechanics that result in ACL injury may aid in the design of effective prevention programs. Purpose: To describe, using video analysis, the mechanisms, situational patterns, and biomechanics of ACL injuries in professional rugby matches. Further aims were to document injuries according to pitch location and timing within the match. Study Design: Case series; Level of evidence, 4. Methods: A total of 62 ACL injuries were identified in players of the 4 most important rugby leagues across 4 consecutive seasons. We analyzed 57 (92%) injury videos for injury mechanism and situational patterns; biomechanical analysis was performed on indirect and noncontact ACL injuries only (38 cases available). Three reviewers independently evaluated each video. Results: More injuries occurred while attacking than defending (41 [72%] vs 16 [28%]; P <.01). Regarding mechanism, 18 (32%) injuries were direct contact; 15 (26%), indirect contact; and 24 (42%), noncontact. Most direct contact injuries involved being tackled directly to the knee (n = 10). Three situational patterns were identified for players who had a noncontact or indirect contact injury: offensive change of direction (COD) (n = 18), being tackled (n = 10), and pressing/tackling (n = 8). Injuries generally involved a knee-loading strategy in the sagittal plane, which was accompanied by knee valgus loading in most cases (94%). Overall, 73% of injuries occurred during the first 40 minutes of effective playing time. Conclusion: Most ACL injuries in professional male rugby players happened through a noncontact or indirect contact mechanism (68%). Three situational patterns were described, including offensive change of direction, being tackled, and pressing/tackling. Biomechanical analysis confirmed a multiplanar mechanism, with a knee-loading pattern in the sagittal plane accompanied by dynamic valgus. As most injuries occurred in the first 40 minutes, accumulated fatigue appears not to be a major risk factor for ACL injury

Circulating endothelial progenitor cells are increased in patients with classic Kaposi’s Sarcoma

Accumulating evidence indicates that tumor angiogenesis is supported by the mobilization and incorporation of endothelial progenitor cells (EPCs), highly proliferative elements derived from the bone marrow. EPCs have been detected at increased frequency in the circulation of patients with different types of cancer. Circulating EPCs have never been quantified in patients with Kaposi’s sarcoma (KS), an angioproliferative malignancy characterized by typical spindle-shaped tumor cells of endothelial origin. In this study, we analyzed the number of EPCs in the peripheral blood of 29 patients with classic KS (cKS) compared with 27 matched healthy controls. EPCs were measured by flow cytometry on fresh blood samples at a single time point. The number of circulating EPCs was significantly higher in cKS patients than controls. EPC count did not correlate with the plasmatic levels of the main proangiogenic factors possibly involved in EPC proliferation and mobilization, thus suggesting that the increased number of EPCs in cKS patients may rather be related to direct or indirect effects of viral environment sustained by HHV-8, the causative agent for KS. The increase of EPCs was significantly more pronounced in cKS patients with slowly evolving than rapidly evolving disease, likely reflecting a localization of EPCs within the lesions during the active phases of KS. Overall, this study demonstrates that EPCs are increased in the peripheral blood of cKS patients and may suggest that changes in the number of circulating EPCs may predict disease progression and may therefore be proposed as a biomarker in the follow-up of KS patients

Are interleukin-16 and thrombopoietin new tolls for the in vitro generation of dendritic cells?

The effects of interleukin 16 (IL-16) on dendritic cell (DC) generation from human CD34+ progenitor cells are not known. Here, we show that IL-16 added to a basal cocktail comprised of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-4, Flt-3 ligand (Flt3L), and tumor necrosis factor \u3b1 (TNF-\u3b1) does induce the CD34+ hematopoietic cells to proliferate in vitro and to differentiate into phenotypically and functionally mature DCs. IL-16 exerts this function more efficiently than stem cell factor (SCF) as a control, thrombopoietin (TPO), or IL-16 plus TPO. Moreover, we show that the combination of IL-16 plus TPO induces the generation of tolerogenic DCs, able to induce an anergic state in T cells that persists when T cells are rechallenged with immunogenic DCs. An altered pattern of cytokine production, a reduced expression of the C-type lectin DC-SIGN, and an increased surface expression of the inhibitory molecules immunoglobulin-like transcript 2 (ILT-2), ILT-3, and ILT-4 may all contribute to confer the tolerogenic properties of these DCs. Generation of tolerogenic DCs may aid the exploration of new therapeutic strategies to promote tolerance to autoantigens and prevent disease development. (Blood. 2004;104:4020-4028

Hybrid photonic-bandgap accelerating cavities

In a recent investigation, we studied two-dimensional point-defected photonic bandgap cavities composed of dielectric rods arranged according to various representative periodic and aperiodic lattices, with special emphasis on possible applications to particle acceleration (along the longitudinal axis). In this paper, we present a new study aimed at highlighting the possible advantages of using hybrid structures based on the above dielectric configurations, but featuring metallic rods in the outermost regions, for the design of extremely-high quality factor, bandgap-based, accelerating resonators. In this framework, we consider diverse configurations, with different (periodic and aperiodic) lattice geometries, sizes, and dielectric/metal fractions. Moreover, we also explore possible improvements attainable via the use of superconducting plates to confine the electromagnetic field in the longitudinal direction. Results from our comparative studies, based on numerical full-wave simulations backed by experimental validations (at room and cryogenic temperatures) in the microwave region, identify the candidate parametric configurations capable of yielding the highest quality factor.Comment: 13 pages, 5 figures, 3 tables. One figure and one reference added; minor changes in the tex

Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women

Background/Aims: T-lymphocyte reactivity against viral antigens may represent the only immunological marker of host contact with a virus. Aim of the present study was to investigate whether vertical exposure to hepatitis C virus (HCV) could activate HCV-specific T-cell responses that may represent a biomarker of previous contact with the virus, and possibly contribute to the low rate of vertical HCV transmission. Methods: We studied 28 children born from chronically HCV-infected mothers. HCV-specific activation and proliferation of CD4+-lymphocytes and cytokine production were evaluated in cultures of peripheral blood mononuclear cells (PBMCs) stimulated in vitro with HCV-peptides. Results: HCV-specific CD4+-cell reactivity was observed in 20 out of the 28 children (71%). The proliferation of HCV-specific CD4+-cells was more frequent and vigorous in children than in their mothers. In children, but not in the mothers, activation of CD4+-cells upon stimulation with HCV-peptides was directly correlated with proliferation. Early upon stimulation with HCV-peptides, lymphocytes from children produced lower levels of IL-10 than lymphocytes from the mothers. Conclusions: Vertical exposure to HCV induces the development of viral-specific CD4+-cell-mediated immune responses, possibly endowed with protective function against infection, which may contribute to the low rate of vertical HCV transmission