Retrograde Accretion and Merging Supermassive Black Holes

We investigate whether a circumbinary gas disc can coalesce a supermassive black hole binary system in the centre of a galaxy. This is known to be problematic for a prograde disc. We show that in contrast, interaction with a retrograde circumbinary disc is considerably more effective in shrinking the binary because there are no orbital resonances. The binary directly absorbs negative angular momentum from the circumbinary disc by capturing gas into a disc around the secondary black hole, or discs around both holes if the binary mass ratio is close to unity. In many cases the binary orbit becomes eccentric, shortening the pericentre distance as the eccentricity grows. In all cases the binary coalesces once it has absorbed the angular momentum of a gas mass comparable to that of the secondary black hole. Importantly, this conclusion is unaffected even if the gas inflow rate through the disc is formally super--Eddington for either hole. The coalescence timescale is therefore always $\sim M_2/\dot M$, where $M_2$ is the secondary black hole mass and $\dot M$ the inflow rate through the circumbinary disc.Comment: 8 pages, 4 figures. Accepted for publication in MNRAS. Movies of the simulations can be found at: http://www.astro.le.ac.uk/users/cjn12/RetroBinaryMovies.htm

The importance of glyoxylate in amino acid biosynthesis in plants

1. [14C2] glyoxylate was rapidly metabolized by carrot storage tissues, pea leaves, pea cotyledons, sunflower cotyledons, corn coleoptiles, corn roots and pea roots. In many tissues over 70% of the supplied [14C2]glyoxylate was utilized during the 6hr. experimental periods. 2. In all tissues, the chief products of [14C2]-glyoxylate metabolism were carbon dioxide, glycine and serine. In several of the tissues, there was also a considerable incorporation of the label into the organic acids, particularly into glycollate. 3. Degradations of the labelled serine produced during [14C2]glyoxylate metabolism showed that glyoxylate carbon was incorporated into all three positions of the serine molecule. 4. The results are interpreted as indicating that glyoxylate is utilized by the tissues by pathways involving transamination, transmethylation, reduction and oxidative decarboxylation of the supplied glyoxylate

Folylpolyglutamate synthesis in a methionine auxotroph of Neurospora crassa

Folylpolyglutamate synthesis in a auxotroph of Neurospora crass

Glycine stimulates polyglutamyl folate synthesis in N. crassa

Glycine stimulates polyglutamyl folate synthesi

Basking African striped mice choose warmer locations to heat up: evidence from a field study

International audienc

Comparison between SELEX and single-step selection for the identification of bivalent aptamers for thrombin

The identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation (massively parallel) sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods

Effect of salbutamol on neuromuscular junction function and structure in a mouse model of DOK7 congenital myasthenia.

Congenital myasthenic syndromes (CMS) are characterized by fatigable muscle weakness resulting from impaired neuromuscular transmission. β2-adrenergic agonists are an effective treatment for DOK7-CMS. DOK7 is a component within the AGRN-LRP4-MUSK-DOK7 signalling pathway that is key for the formation and maintenance of the synaptic structure of the neuromuscular junction (NMJ). The precise mechanism of action of β2-adrenergic agonists at the NMJ is not fully understood. In this study, we investigated whether β2-adrenergic agonists improve both neurotransmission and structural integrity of the NMJ in a mouse model of DOK7-CMS. Ex-vivo electrophysiological techniques and microscopy of the NMJ were used to study the effect of salbutamol, a β2-adrenergic agonist, on synaptic structure and function. DOK7-CMS model mice displayed a severe phenotype with reduced weight gain and perinatal lethality. Salbutamol treatment improved weight gain and survival in DOK7 myasthenic mice. Model animals had fewer active NMJs, detectable by endplate recordings, compared with age-matched wild-type littermates. Salbutamol treatment increased the number of detectable NMJs during endplate recording. Correspondingly, model mice had fewer acetylcholine receptor-stained NMJs detected by fluorescent labelling, but following salbutamol treatment an increased number were detectable. The data demonstrate that salbutamol can prolong survival and increase NMJ number in a severe model of DOK7-CMS

Screening for coping style increases the power of gene expression studies

Background: Individuals of many vertebrate species show different stress coping styles and these have a striking influence on how gene expression shifts in response to a variety of challenges. Principal Findings: This is clearly illustrated by a study in which common carp displaying behavioural predictors of different coping styles (characterised by a proactive, adrenaline-based or a reactive, cortisol-based response) were subjected to inflammatory challenge and specific gene transcripts measured in individual brains. Proactive and reactive fish differed in baseline gene expression and also showed diametrically opposite responses to the challenge for 80% of the genes investigated. Significance: Incorporating coping style as an explanatory variable can account for some the unexplained variation that is common in gene expression studies, can uncover important effects that would otherwise have passed unnoticed and greatly enhances the interpretive value of gene expression data