Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress

Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3¿-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed map

Market Power and Patent Strategies: Evidence from Renaissance Venice

This paper exploits the introduction of the first regularized patent system, which appeared in the Venetian Republic in 1474, to examine the factors shaping inventors\u2019 propensity to use a new form of intellectual property. We combine detailed information on craft guilds and patents in Renaissance Venice and show a negative association between patenting activity and guild statutory norms that strongly restricted entry and price competition. Our analysis shows that the heterogeneity in patenting activity documented by the industrial organization literature is not a special feature of modern technologies, but is rather a persistent phenomenon affected by market power

Application of the socio-ecological system framework to forest fire risk management: A systematic literature review

Although increasing concern about climate change has raised awareness of the fundamental role of forest ecosystems, forests are threatened by human-induced impacts worldwide. Among them, wildfire risk is clearly the result of the interaction between human activities, ecological domains, and climate. However, a clear understanding of these interactions is still needed both at the global and local levels. Numerous studies have proven the validity of the socioecological system (SES) approach in addressing this kind of interdisciplinary issue. Therefore, a systematic review of the existing literature on the application of SES frameworks to forest ecosystems is carried out, with a specific focus on wildfire risk management. The results demonstrate the existence of different methodological approaches that can be grouped into seven main categories, which range from qualitative analysis to quantitative spatially explicit investigations. The strengths and limitations of the approaches are discussed, with a specific reference to the geographical setting of the works. The research suggests the importance of local community involvement and local knowledge consideration in wildfire risk management. This review provides a starting point for future research on forest SES and a supporting tool for the development of a sustainable wildfire risk adaptation and mitigation strategy

The reintroduction of Castor fiber in Piedmont (Italy): An integrated SWOT-spatial multicriteria based approach for the analysis of suitability scenarios

The Castor fiber or Eurasian beaver can change its habitat by building dams and creating ponds. For this reason, Castor fiber is known as an “ecosystem engineer” for aquatic and riparian environments. Despite its ecological importance, at the beginning of the 20th century the population was reduced to only 1200 beavers in Europe and Asia, due to uncontrolled hunting. Recently, some reintroductions and translocations have partly re-established the population. In Italy, however, the beaver disappeared in the 16th century and no action has been taken despite the recommendation of the Council of Europe to perform a feasibility study. This research evaluates beaver reintroduction and identifies suitable areas in Italy and, in particular, in the Piedmont region. In order to achieve this, a SWOT analysis combined with a Spatial Multicriteria Analysis was performed. Firstly, the zoological and ethological aspects concerning this rodent were studied, as well as the historical reasons that led to its disappearance in Italy and near extinction in Eurasia. Secondly, Strengths, Weaknesses, Opportunities and Threats (SWOT) of the territory were identified for beaver reintroduction. The SWOT analysis was implemented, as the starting point for the spatial multicriteria analysis. Thirdly, the Multicriteria Spatial Decision Support System (MC-SDSS) was structured into two criteria, i.e. Potentials and Criticalities, representing the spatialization of strengths and weaknesses. The final result of the MC-SDSS is a map showing suitable areas for beaver reintroduction in Piedmont. This map is the weighted sum of the maps of criticalities and potentialities, performed through a set of GIS operations and weighted through a pairwise comparison of criteria by experts. The analysis was conducted for the Piedmont region, but the integrated approach and the set of criteria can also be applied in other regions. Moreover, this mixed-method approach takes into account the characteristics necessary for the choice of suitable beaver habitats and also includes economic and social aspects. Therefore, it is an improvement on the Habitat Suitability Index (HIS), generally used in reintroductions. The aspects considered in the analysis are fundamental for the future development of a shared action plan, which considers both technical and social motivations and acts for the long-term on a wide area

Biogas production by anaerobic co-digestion of cattle slurry and cheese whey

Biogas yield of mixtures of cattle slurry and cheese whey, rates of production of methane, removal efficiencies of chemical oxygen demand (COD) and biological oxygen demand (BOD) were investigated at 35 C. Stable biogas production of 621 l/kg volatile solids at a hydraulic retention time of 42 days in a mixture containing 50% slurry and whey was obtained. The concentration of methane in the biogas was around 55%. Maximum removal efficiencies for COD and BOD5 were 82% and 90%, respectively. A maximum biogas production increase of 79% with respect to the start-up phase was achieved. The result of this study show that co-digestion of a high volume of whey (up to 65% in volume) is possible without the use of chemicals for pH correction, but also that this kind of mix has a similar energetic potential for anaerobic digestion as energy crops such as maiz

Role of rock fragment cover on runoff generation and sediment yield in tilled vineyards.

The soil in conventional Mediterranean vineyards is an active and non-sustainable source of sediment and water. Lack of vegetation cover, small soil organic matter content and intense ploughing result in large rates of erosion in a millennia-old tillage system. There is a need for soil conservation strategies that enable sustainability of wine and grape production; therefore, it is essential to measure the rates and to investigate the processes and factors of soil erosion. This study evaluated factors that can reduce soil losses in traditional Mediterranean vineyards. The investigation was carried out with 96 rainfall simulation experiments at the pedon scale (0.24m2) to measure soil detachment and runoff yield under low frequency-high magnitude rainfall events of 1 hour at 55mmhour−1. On average, runoff was 40.6% of the rainfall, and the rate of soil erosion (i.e. amount of soil lost) was 71.5 g m−2. The key factor controlling erosion was the rock fragment cover. There was a clear decrease in soil losses with increased rock fragment cover on the soil surface, but an increase in surface runoff. The results of our study showed that rock fragments at the pedon scale reduced soil erosion in Mediterranean vineyards, but when a layer of embedded rock fragments developed, large rates of runoff were triggered

Factors determining the soil available water during the last two decades (1997–2019) in southern Spain

Funding for open access charge: Universidad de Granada/CBUA. This study was part of the research project "Incidence of global change in contrasted Mediterranean landscapes," GLOMED-LAND (CSO2016-75898-P), funded by the Ministry of Economy and Competitiveness.Assessing soil hydrological conditions can provide essential information for understanding the environmental processes that affect ecosystem services and, particularly in the context of ongoing climate change. This is key in areas affected by water scarcity such as the Mediterranean belt. Therefore, the main goals of this research are (i) to assess the main rainfall dynamics and trends of some representative hotspots along with southern Spain and (ii) to determine the impact on the soil available water content (AWC) over the last two decades. An analysis of daily precipitation and soil hydrological conditions was combined with soil sampling (543) and laboratory analyses to evaluate the properties related to the soil infiltration and retention capacity. The results show that the organic factors control soil properties and their hydrodynamics in southern Spain. Furthermore, a general declining trend in soil water availability is observed over the last two decades. This is more extreme in arid and semi-arid areas, where there have been several years in the last decade with more than 200 days without the available water content. Moreover, in these areas, heavy rainfall during specific moments of the year is the key factor that manifests a greater incidence in areas with steeper slopes, which in turn, also conditions the biological factors and the hydrodynamics of the soil. In short, in the context of climate change, the analysis of soil hydrological dynamics could be used to identify biodiversity thresholds in the Mediterranean area and even to detect phenological changes in specific plant species.Ministry of Economy and Competitiveness CSO2016-75898-