Improving the quality of Portuguese SMEs through competence evaluation

UIDB/04647/2020 UIDP/04647/2020Problem and goal. Since we are going through a key moment in terms of technological advances and their effect on the labour field, the investigation of the on-the-job training processes gains a predominant role. Moreover, the need to research an approach that promotes a balance between the four spheres of development of learning – economic, sustain-able, community and personal – is emerging. This study was conceived as a result of this ur-gency, aiming to respond to the following problem: to what extent does a training model, based on the VPL (Validation of Prior Learning) approach and supported by principles of the educational sciences, can contribute to individual empowerment and, consequently, to an improvement in the organizational quality of Portuguese SMEs (Small and Medium-sized Enterprises)? Methodology. The division of the research into two parts – Study 1 and Study 2 – al-lowed the collection and analysis of structural data (Study 1) that served as a basis for the defi- nition of a dynamic strategy (Study 2). Although undertaken at different times, they have thus proved to be complementary. In fact, the design of the training model was based, firstly, on the results obtained through exploratory interviews with key informants and, secondly, on the data obtained throughout the training process that involved ninety-two employees from ten Portuguese SMEs from diverse categories and sectors. Results. The training model, which emerged from the interaction between the theoreti-cal reflection and the practical context, comprises five components: distance learning, recog-nition of skills, language skills, intercultural skills and an individual action plan. Conclusion. This study, being an important contribution to the topic of on-the-job training in Portuguese SMEs, at the same time opens the way for the continuation of the research.publishersversionpublishe

The relative abundance of wheat Rubisco activase isoforms is post‑transcriptionally regulated

Diurnal rhythms and light availability affect transcription–translation feedback loops that regulate the synthesis of photosynthetic proteins. The CO2-fixing enzyme Rubisco is the most abundant protein in the leaves of major crop species and its activity depends on interaction with the molecular chaperone Rubisco activase (Rca). In Triticum aestivum L. (wheat), three Rca isoforms are present that differ in their regulatory properties. Here, we tested the hypothesis that the relative abundance of the redox-sensitive and redox-insensitive Rca isoforms could be differentially regulated throughout light–dark diel cycle in wheat. While TaRca1-β expression was consistently negligible throughout the day, transcript levels of both TaRca2-β and TaRca2-α were higher and increased at the start of the day, with peak levels occurring at the middle of the photoperiod. Abundance of TaRca-β protein was maximal 1.5 h after the peak in TaRca2-β expression, but the abundance of TaRca-α remained constant during the entire photoperiod. The redox-sensitive TaRca-α isoform was less abundant, representing 85% of the redox-insensitive TaRca-β at the transcript level and 12.5% at the protein level. Expression of Rubisco large and small subunit genes did not show a consistent pattern throughout the diel cycle, but the abundance of Rubisco decreased by up to 20% during the dark period in fully expanded wheat leaves. These results, combined with a lack of correlation between transcript and protein abundance for both Rca isoforms and Rubisco throughout the entire diel cycle, suggest that the abundance of these photosynthetic enzymes is post-transcriptionally regulated

Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis

Rubisco is a major target for improving crop photosynthesis and yield, yet natural diversity in catalytic properties of this enzyme is poorly understood. Rubisco from 25 genotypes of the Triticeae tribe, including wild relatives of bread wheat (Triticum aestivum), were surveyed to identify superior enzymes for improving photosynthesis in this crop. In vitro Rubisco carboxylation velocity (V c), Michaelis–Menten constants for CO2 (K c) and O2 (K o) and specificity factor (S c/o) were measured at 25 and 35 °C. V c and K c correlated positively, while V c and S c/o were inversely related. Rubisco large subunit genes (rbcL) were sequenced, and predicted corresponding amino acid differences analysed in relation to the corresponding catalytic properties. The effect of replacing native wheat Rubisco with counterparts from closely related species was analysed by modelling the response of photosynthesis to varying CO2 concentrations. The model predicted that two Rubisco enzymes would increase photosynthetic performance at 25 °C while only one of these also increased photosynthesis at 35 °C. Thus, under otherwise identical conditions, catalytic variation in the Rubiscos analysed is predicted to improve photosynthetic rates at physiological CO2 concentrations. Naturally occurring Rubiscos with superior properties amongst the Triticeae tribe can be exploited to improve wheat photosynthesis and crop productivity

Persistence of Left Superior Vena Cava Associated to Esophageal Atresia: Presentation of 5 Cases

Objectivos: A atresia do esófago está associada a uma variedade de malformações congénitas de outros órgãos. A persistência da veia cava superior esquerda e uma das malformações venosas torácicas mais comuns, ocorrendo em 0,3% da população. O objectivo deste estudo é caracterizar a persistência da veia cava superior esquerda nos doentes com atresia do esófago tratados no Hospital Dona Estefânia. Métodos: Estudo retrospectivo dos doentes admitidos por atresia do esófago desde Janeiro 2002 a Dezembro 2013. As seguintes variáveis foram estudadas, considerando, idade gestacional, o peso à nascença, o sexo, tipo de atresia do esófago, ecocardiograma no pré-operatório, malformações associadas, abordagem cirúrgica, eventual ecocardiograma no pós-operatório e angioressonância. Resultados: De um total de 107 doentes, em cinco casos foi diagnosticada persistência da veia cava superior esquerda. Destes, apenas um tinha diagnostico pré-natal. A restante investigação revelou um doente com atresia duodenal e outro com malformação do aparelho urinário, coloboma e malformação bilateral dos pavilhões auriculares. Todos os cinco doentes foram submetidos a toracotomia, esofagoesofagostomia primária após laqueação da fístula traqueo-esofágica e angio-ressonância para caracterizar a malformação vascular. Não houve registo de complicações, quer intra-operatórias quer pós-operatórias. Conclusão: Apesar do estudo pré-operatório, apenas se diagnosticou persistência da veia cava superior esquerda num dos doentes. O diagnóstico é geralmente feito de forma incidental durante a colocação rotineira de catéteres venosos centrais à esquerda. É importante identificar estes doentes e caracterizar o seu padrão de retorno venoso cardíaco, pelo risco de complicações embó1icas paradoxais para o sistema arterial

Characterization of natural cork agglomerate functionalised by plasma treatment

This work intends to study the chemical and physical modifications of natural cork agglomerate after plasma treatment using dielectric barrier discharge (DBD). Different experimental techniques were used to evaluate the surface alterations of the substrate pre- treated with DBD plasma, namely, static and dynamic contact angle, surface energy, Fourier transform infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS), scanning electronic microscopy (SEM) and differential scanning calorimetry (DSC). Plasma discharge greatly increases the wettability and surface energy of the samples. Chemical and physical analyses of the cork agglomerate confirmed considerable surface modification.The authors gratefully acknowledge the CsF – CNPq and CAPES Foundation, Brazil, for the doctoral grants 202539/2011-3 and BEX0978/12-4. We would also like to thank the Project no 2011/19280 (“COLTEC”), FCT and FEDER-COMPETE funding PEt-C/CTM/UI0264/2011

Functionalization of cork agglomerate composite with pcm microcapsules after dbd plasma treatment

This research intends to study the influence of dielectric barrier discharge (DBD) plasma treatment on the adsorption of Phase Change Materials (PCM) microcapsules applied to cork agglomerate laminated with a polymer membrane. Several experimental techniques were used to evaluate cork and membrane surface modification after plasma treatment and the influence on the microcapsules adsorption, namely, Static and Dynamic Contact Angle, Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM) and Tensile Strength. The plasma treatment greatly increases the hydrophilicity of both materials, justifying that more microcapsules are adsorbed on this composite. Chemical and physical characterization of the cork agglomerate and polymer membrane confirmed significant surface alteration.The authors gratefully acknowledge the financial support from - Programme CsF–CNPq and CAPES Foundation, Brazil, for the doctoral grants 202539/2011-3 and BEX0978/12-4. The authors would also like to express their acknowledgment to FCT and FEDER-COMPETE funding, under the project PEst-C/CTM/UI0264/2011

Obesity-related knowledge and body mass index : a national survey in Portugal

© 2019 Springer Nature Switzerland AG. Part of Springer Nature.Purpose: Obesity-related knowledge predicts weight control but previous studies only comprise individuals with excessive weight and assess very specific aspects of knowledge. This study aims to evaluate several domains of obesity-related knowledge according to the body mass index (BMI) in a representative sample of Portuguese-speaking dwellers in mainland Portugal. Methods: A sample of 1624 participants aged 16–79 years was analyzed. Eighteen questions comprising eight obesity domains were asked: prevalence, physical activity, number of calories, adiposity location, causes, diagnosis, treatment and consequences; each one was dichotomized into correct/incorrect knowledge. Results: The majority of Portuguese-speaking dwellers recognized the benefits of physical activity, the risks of abdominal obesity and most consequences of excessive weight, independently of their BMI. However, knowledge gaps were identified regarding prevalence, calories and BMI diagnosis. BMI influenced specific obesity-related knowledge: participants with a normal BMI knew the BMI formula more often, identified 22 as normal BMI more frequently and had the highest proportion of correct knowledge regarding the number of calories an adult should eat; obese individuals identified natural products as not being good treatments for obesity more often. After adjusting for age, sex and educational level, obese individuals identified natural products and supplements as not being good obesity treatments more often. Conclusions: Obesity-related knowledge gaps (prevalence, calories, and diagnosis) were identified among Portuguese adults. Moreover, correct knowledge does not necessarily translate into a healthier BMI. Besides the dissemination of accurate information, public health interventions should focus on the transfer of knowledge to behaviors that will guarantee better weight management. Evidence-based medicine rankings: Level V: Opinions of respected authorities, based on descriptive studies, narrative reviews, clinical experience, or reports of expert committees.This study was supported by FEDER funds through Operational Programme Competitiveness and Internationalization and by national funding from The Portuguese Foundation for Science and Technology—FCT (Portuguese Ministry of Education and Science) within the project (HMSP-IISE/SAU-ICT/0004/2009) and within the Epidemiology Research Unit—Institute of Public Health, University of Porto (EPIUnit) (POCI-01-0145-FEDER-006862; Ref. UID/ DTP/04750/2013). Additionally, we gratefully acknowledge the FCT Investigator contract IF/01674/2015 (Susana Silva) and also a Scientifc Employment Stimulus contract CEECIND/01793/2017 (Ana Henriques). The funding source had no role in the study design, collection, analysis, or interpretation of data, writing the manuscript, or the decision to submit the manuscript for publication.info:eu-repo/semantics/publishedVersio

Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency.

The threat to global food security of stagnating yields and population growth makes increasing crop productivity a critical goal over the coming decades. One key target for improving crop productivity and yields is increasing the efficiency of photosynthesis. Central to photosynthesis is ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco, which is a critical but often rate-limiting component. Here we present full Rubisco catalytic properties measured at three temperatures for 75 plants species representing both crops and undomesticated plants from diverse climates. Some newly characterised Rubiscos were naturally 'better' compared to crop enzymes and have the potential to improve crop photosynthetic efficiency. The temperature response of the various catalytic parameters was largely consistent across the diverse range of species, though absolute values showed significant variation in Rubisco catalysis, even between closely related species. An analysis of residue differences amongst the species characterised identified a number of candidate amino acid substitutions that will aid in advancing engineering of improved Rubisco in crop systems. This study provides new insights on the range of Rubisco catalysis and temperature response present in nature, and provides new information to include in models from leaf to canopy and ecosystem scale

Photonic sensors based on flexible materials with FBGs for use on biomedical applications

This chapter is intended for presenting biomedical applications of FBGs embedded into flexible carriers for enhancing the sensitivity and to provide interference-free instrumentation.This work was fully supported by the Algoritmi’s Strategic Project UI 319-2011-2012, under the Portuguese Foundation for Science and Technology grant Pest C/EEI/UI0319/2011