Quercetin solubilisation in bile salts: A comparison with sodium dodecyl sulphate

AbstractTo understand the bioaccessibility of the flavonoid quercetin we studied its interaction with bile salt micelles. The environmental sensitivity of quercetin’s UV–visible absorption spectrum gave information about quercetin partitioning. Two quercetin absorption peaks gave complementary information: Peak A (240–280nm) on the intermicellar phase and Peak B (340–440nm) on the micellar phase. Thus, by altering pH, we showed that only non-ionised quercetin partitions into micelles. We validated our interpretation by studying quercetin’s interaction with SDS micelles. Pyrene fluorescence and the quercetin UV–visible spectra show that the adsorption site for pyrene and quercetin in bile salt micelles is more hydrophobic than that for SDS micelles. Also, both quercetin and pyrene reported a higher critical micelle concentration for bile salts than for SDS. Our method of using a flavonoid as an intrinsic probe, is generally applicable to other lipophilic bioactives, whenever they have observable environmental dependent properties

The effects of type of discourse and of a second language on comprehension processes : a new linguistic relativism?

A través de este trabajo se examinan diversas cuestiones relacionadas con los procesos de comprensión del discurso escrito y de la adquisición de segundas lenguas como el español, inglés o portugués. El objetivo fundamental de este artículo es tratar de desvelar si el procesamiento que realiza el lector durante la comprensión de un discurso determinado o ante la adquisición de una segunda lengua requiere de una actividad cognitiva diferente a cuando se trata de comprender otro de naturaleza diferente o bajo una lengua diferente. Para ello se analiza por un lado, las diferencias existentes entre diferentes tipos de texto, centrándonos fundamentalmente en el narrativo y el expositivo, analizando sus repercusiones sobre varios niveles de la cognición humana. Por otro, se analizan algunos estudios sobre la adquisición de diferentes segundas lenguas y sus repercusiones sobre su nivel de activación cerebral utilizando la técnica de fMRI. Por último, se plantea hasta qué punto el relativismo lingüístico influye en los procesos de comprensión y de la realización de inferencias y de la adquisición de una segunda lengua.In this paper we address issues related to comprehension processes, and how they can be affected by types of text or written discourse, and by a reader’s first or second language. To this end, we discuss the processing of narrative and expository texts, and their effects on different levels of human cognition. Likewise, we discuss the effects of texts written in different codes, that is, in first and second languages. We propose that differences in text comprehension processes occur as a function of their causal nature and of the inferences that are generated during comprehension. In terms of second language comprehension, modern research applying functional magnetic resonance imaging, or fMRI, with bilinguals is discussed in support of the notion that first language comprehension processes are generally replicated in the second language. Therefore, we focus on comprehension processes, generation of interferences, and the processing of a second language as influenced by linguistic relativism

Caracterização agrônomica de populações locais de azevém na região sul do Brasil.

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Arithmetically Cohen-Macaulay Bundles on complete intersection varieties of sufficiently high multidegree

Recently it has been proved that any arithmetically Cohen-Macaulay (ACM) bundle of rank two on a general, smooth hypersurface of degree at least three and dimension at least four is a sum of line bundles. When the dimension of the hypersurface is three, a similar result is true provided the degree of the hypersurface is at least six. We extend these results to complete intersection subvarieties by proving that any ACM bundle of rank two on a general, smooth complete intersection subvariety of sufficiently high multi-degree and dimension at least four splits. We also obtain partial results in the case of threefolds.Comment: 15 page

D-brane Categories for Orientifolds -- The Landau-Ginzburg Case

We construct and classify categories of D-branes in orientifolds based on Landau-Ginzburg models and their orbifolds. Consistency of the worldsheet parity action on the matrix factorizations plays the key role. This provides all the requisite data for an orientifold construction after embedding in string theory. One of our main results is a computation of topological field theory correlators on unoriented worldsheets, generalizing the formulas of Vafa and Kapustin-Li for oriented worldsheets, as well as the extension of these results to orbifolds. We also find a doubling of Knoerrer periodicity in the orientifold context.Comment: 45 pages, 6 figure

Characterizing normal crossing hypersurfaces

The objective of this article is to give an effective algebraic characterization of normal crossing hypersurfaces in complex manifolds. It is shown that a hypersurface has normal crossings if and only if it is a free divisor, has a radical Jacobian ideal and a smooth normalization. Using K. Saito's theory of free divisors, also a characterization in terms of logarithmic differential forms and vector fields is found and and finally another one in terms of the logarithmic residue using recent results of M. Granger and M. Schulze.Comment: v2: typos fixed, final version to appear in Math. Ann.; 24 pages, 2 figure

Visualizing metabolic network dynamics through time-series metabolomic data.

BACKGROUND: New technologies have given rise to an abundance of -omics data, particularly metabolomic data. The scale of these data introduces new challenges for the interpretation and extraction of knowledge, requiring the development of innovative computational visualization methodologies. Here, we present GEM-Vis, an original method for the visualization of time-course metabolomic data within the context of metabolic network maps. We demonstrate the utility of the GEM-Vis method by examining previously published data for two cellular systems-the human platelet and erythrocyte under cold storage for use in transfusion medicine. RESULTS: The results comprise two animated videos that allow for new insights into the metabolic state of both cell types. In the case study of the platelet metabolome during storage, the new visualization technique elucidates a nicotinamide accumulation that mirrors that of hypoxanthine and might, therefore, reflect similar pathway usage. This visual analysis provides a possible explanation for why the salvage reactions in purine metabolism exhibit lower activity during the first few days of the storage period. The second case study displays drastic changes in specific erythrocyte metabolite pools at different times during storage at different temperatures. CONCLUSIONS: The new visualization technique GEM-Vis introduced in this article constitutes a well-suitable approach for large-scale network exploration and advances hypothesis generation. This method can be applied to any system with data and a metabolic map to promote visualization and understand physiology at the network level. More broadly, we hope that our approach will provide the blueprints for new visualizations of other longitudinal -omics data types. The supplement includes a comprehensive user\u27s guide and links to a series of tutorial videos that explain how to prepare model and data files, and how to use the software SBMLsimulator in combination with further tools to create similar animations as highlighted in the case studies

Quiver GIT for varieties with tilting bundles

In the setting of a variety X admitting a tilting bundle T we consider the problem of constructing X as a quiver GIT quotient of the algebra A:=EndX(T)opA:=EndX(T)op . We prove that if the tilting equivalence restricts to a bijection between the skyscraper sheaves of X and the closed points of a quiver representation moduli functor for A=EndX(T)opA=EndX(T)op then X is indeed a fine moduli space for this moduli functor, and we prove this result without any assumptions on the singularities of X. As an application we consider varieties which are projective over an affine base such that the fibres are of dimension 1, and the derived pushforward of the structure sheaf on X is the structure sheaf on the base. In this situation there is a particular tilting bundle on X constructed by Van den Bergh, and our result allows us to reconstruct X as a quiver GIT quotient for an easy to describe stability condition and dimension vector. This result applies to flips and flops in the minimal model program, and in the situation of flops shows that both a variety and its flop appear as moduli spaces for algebras produced from different tilting bundles on the variety. We also give an application to rational surface singularities, showing that their minimal resolutions can always be constructed as quiver GIT quotients for specific dimension vectors and stability conditions. This gives a construction of minimal resolutions as moduli spaces for all rational surface singularities, generalising the G-Hilbert scheme moduli space construction which exists only for quotient singularities

On the monoidal structure of matrix bi-factorisations

We investigate tensor products of matrix factorisations. This is most naturally done by formulating matrix factorisations in terms of bimodules instead of modules. If the underlying ring is C[x_1,...,x_N] we show that bimodule matrix factorisations form a monoidal category. This monoidal category has a physical interpretation in terms of defect lines in a two-dimensional Landau-Ginzburg model. There is a dual description via conformal field theory, which in the special case of W=x^d is an N=2 minimal model, and which also gives rise to a monoidal category describing defect lines. We carry out a comparison of these two categories in certain subsectors by explicitly computing 6j-symbols.Comment: 43 pages; v2: corrected a mistake in sec. 1 and app. A.1, the results are unaffected; v3: minor change