El uso de las redes sociales para la gestión de la reputación de marca: El caso de Antena 3 Noticias

El objetivo de este trabajo es analizar en qué medida el uso de las redes sociales por parte de los servicios informativos de una cadena de televisión generalista de ámbito nacional supone una ventaja competitiva desde la perspectiva de la gestión de su propia reputación. Para ello se analizará en primer lugar el contexto de los medios de comunicación en España desde el cambio de siglo (capítulo 1), y se profundizará especialmente en el sector televisivo (capítulo 2). De forma inductiva, se constatará que uno de los elementos clave para la gestión de la transición estructural que viven hoy los medios de comunicación, tras el cambio de paradigma producido por la consolidación de Internet como principal canal de información y entretenimiento, es la reputación de su marca, y se analizará el concepto de marca empresarial y de su reputación (capítulo 3)

El uso de las redes sociales para la gestión de la reputación de marca: El caso de Antena 3 Noticias

El objetivo de este trabajo es analizar en qué medida el uso de las redes sociales por parte de los servicios informativos de una cadena de televisión generalista de ámbito nacional supone una ventaja competitiva desde la perspectiva de la gestión de su propia reputación. Para ello se analizará en primer lugar el contexto de los medios de comunicación en España desde el cambio de siglo (capítulo 1), y se profundizará especialmente en el sector televisivo (capítulo 2). De forma inductiva, se constatará que uno de los elementos clave para la gestión de la transición estructural que viven hoy los medios de comunicación, tras el cambio de paradigma producido por la consolidación de Internet como principal canal de información y entretenimiento, es la reputación de su marca, y se analizará el concepto de marca empresarial y de su reputación (capítulo 3)

Analysis of gene transcription alterations at the blastocyst stage related to the long-term consequences of in vitro culture in mice

We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the presence of FCS produces long-term effects (LTE) on development, growth and behaviour of the offspring at adult age. To analyse the mechanisms underlying this phenomenon, we have examined development and global alterations in gene expression in the mouse blastocysts produced in the presence of FCS, conditions known to be suboptimal and that generate LTE. Embryos cultured in vitro in KSOM and in KSOMC+FCS had a reduced number of cells in the inner cell mass at the blastocyst stage compared with in vivo derived embryos; however, only culture in KSOM+FCS leads to a reduction in the number of trophoblast cells. Gene expression levels were measured by comparison among three groups of blastocysts (in vivo, IVC in KSOM and IVC in KSOM+FCS). Different patterns of gene expression and development were found between embryos cultured in vitro or in vivo. Moreover, when we compared the embryos produced in KSOM versus KSOM+FCS, we observed that the presence of FCS affected the expression of 198 genes. Metabolism, proliferation, apoptosis and morphogenetic pathways were the most common processes affected by IVC. However, the presence of FCS during IVC preferentially affected genes associated with certain molecular and biological functions related to epigenetic mechanisms. These results suggest that culture-induced alterations in transcription at the blastocyst stage related to epigenetic mechanisms provide a foundation for understanding the molecular origin at the time of preimplantation development of the long-term consequences of IVC in mammals. © 2009 Society for Reproduction and Fertility

Spaceflight-related suboptimal conditions can accentuate the altered gravity response of Drosophila transcriptome

Genome-wide transcriptional profiling shows that reducing gravity levels during Drosophila metamorphosis in the International Space Station (ISS) causes important alterations in gene expression: a large set of differentially expressed genes (DEGs) are observed compared to 1g controls. However, the preparation procedures for spaceflight and the nonideal environmental conditions on board the ISS subject the organisms to additional environmental stresses that demonstrably affect gene expression. Simulated microgravity experiments performed on the ground, under ideal conditions for the flies, using the random position machine (RPM), show much more subtle effects on gene expression. However, when the ground experiments are repeated under conditions designed to reproduce the additional environmental stresses imposed by spaceflight procedures, 79% of the DEGs detected in the ISS are reproduced by the RPM experiment. Gene ontology analysis of them shows they are genes that affect respiratory activity, developmental processes and stress-related changes. Here, we analyse the effects of microgravity on gene expression in relation to the environmental stresses imposed by spaceflight. Analysis using ‘gene expression dynamics inspector’ (GEDI) self-organizing maps reveals a subtle response of the transcriptome to microgravity. Remarkably, hypergravity simulation induces similar response of the transcriptome, but in the opposite direction, i.e. the genes promoted under microgravity are usually suppressed under hypergravity. These results suggest that the transcriptome is finely tuned to normal gravity and that microgravity, together with environmental constraints associated with space experiments, can have profound effects on gene expression

NGFR regulates stromal cell activation in germinal centers

International audienceNerve growth factor receptor (NGFR) is expressed by follicular dendritic cells (FDCs). However, the role of NGFR in the humoral response is not well defined. Here, we study the effect of Ngfr loss on lymph node organization and function, demonstrating that Ngfr depletion leads to spontaneous germinal center (GC) formation and an expansion of the GC B cell compartment. In accordance with this effect, stromal cells are altered in Ngfr(-/-) mice with a higher frequency of FDCs, characterized by CD21/35, MAdCAM-1, and VCAM-1 overexpression. GCs are located ectopically in Ngfr(-/-) mice, with lost polarization together with impaired high-affinity antibody production and an increase in circulating autoantibodies. We observe higher levels of autoantibodies in Bcl2 Tg/Ngfr(-/-) mice, concomitant with a higher incidence of autoimmunity and lower overall survival. Our work shows that NGFR is involved in maintaining GC structure and function, participating in GC activation, antibody production, and immune tolerance

Co-option of neutrophil fates by tissue environments

Classically considered short-lived and purely defensive leukocytes, neutrophils are unique in their fast and moldable response to stimulation. This plastic behavior may underlie variable and even antagonistic functions during inflammation or cancer, yet the full spectrum of neutrophil properties as they enter healthy tissues remains unexplored. Using a new model to track neutrophil fates, we found short but variable lifetimes across multiple tissues. Through analysis of the receptor, transcriptional, and chromatin accessibility landscapes, we identify varying neutrophil states and assign non-canonical functions, including vascular repair and hematopoietic homeostasis. Accordingly, depletion of neutrophils compromised angiogenesis during early age, genotoxic injury, and viral infection, and impaired hematopoietic recovery after irradiation. Neutrophils acquired these properties in target tissues, a process that, in the lungs, occurred in CXCL12-rich areas and relied on CXCR4. Our results reveal that tissues co-opt neutrophils en route for elimination to induce programs that support their physiological demands