62 research outputs found

    Unpredictable Variable Prenatal Stress Programs Expression of Genes Involved in Appetite Control and Energy Expenditure

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    Exposure to stress in the womb shapes neurobiological and physiological outcomes of offspring in later life, including body weight regulation and metabolic profiles. Our previous work utilizing a centrifugation-induced hypergravity demonstrated significantly increased (8-15) body mass in male, but not female, rats exposed throughout gestation to chronic 2-g from conception to birth. We reported the same outcome in adult offspring exposed throughout gestation to Unpredictable Variable Prenatal Stress (UVPS). Here we examine gene expression changes using our UVPS model to identify a potential role for prenatal stress in this hypergravity programming effect. Specifically we focused on appetite control and energy expenditure pathways in prenatally stressed adult (90-day-old) male Sprague-Dawley rats. Time-mated female rats were exposed throughout their 22-day pregnancy to UVPS consisting of white noise, strobe light, and tube restraint individually once per day on an unpredictable schedule for 15, 30 or 60 min. To control for potential changes in postnatal maternal care, newborn pups were fostered to non-manipulated, newly parturient dams. At 90-days of age, we analyzed plasma concentrations of hormones involved in appetite control and energy expenditure (leptin and adiponectin), and quantified expression of key genes in epididymal fat pads harvested from adult male offspring and controls. Leptin regulates energy balance by inhibiting hunger, and adiponectin modulates glucose levels and fatty acid breakdown. Our findings indicate significantly elevated plasma leptin concentrations and reduced expression of epididymal fat leptin (OB) and adiponectin (ADIPOQ) genes compared to controls. Analyses presently underway include quantification of plasma insulin and glucose, and the expression of ghrelin, a peptide that acts on the central nervous system and the body's perception of hunger. Collectively, these findings will further understanding of the consequences of UVPS on body weight regulation and metabolism, and provide further insight into the effect of gravity modulation on mammalian fetal development

    A comparative assessment of the amount and rate of orthodontic space closure toward a healed vs recent lower premolar extraction site.

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    To investigate and compare the amount and rate of space closure and tooth tipping during orthodontic space closure toward a recent vs healed first premolar extraction site. The mandibular arches of 23 patients were included. Treatment plans included lower first premolar extractions. After reaching 0.019 × 0.025-inch stainless-steel archwires (SSAW), patients were subdivided into two groups (Group 1: space closure was carried out toward a healed first premolar extraction space and Group 2: space closure was carried out immediately after first premolar extraction). Elastomeric power chain from second molar to second molar was used to close lower extraction spaces. The following time points were defined: T1: just before space closure; T2-T4: 1-3 months after initial space closure. Records consisted of dental study models. The amount and rate of extraction space closure were evaluated at each time point. In Group 1 (healed socket), a total amount of 1.98 mm (coronally) and 1.75 mm (gingivally) of space closure was achieved. The rate of space closure was 0.66 mm/month coronally and 0.58 mm/month gingivally. In Group 2 (recent socket), the total amount of space closure was 3.02 mm coronally and 2.68 mm gingivally. The rate of space closure was 1.01 mm/month coronally and 0.89 mm/month gingivally. Differences between the two groups were significant (P .05). In the lower arch, the amount and rate of space closure toward a recent extraction site were higher than that toward a healed extraction socket with similar tipping of teeth in both groups.This study was supported by the Deanship of Research/Jordan University of Science and Technology (Grant number 53/2019)

    Clades of huge phages from across Earth's ecosystems

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    Bacteriophages typically have small genomes and depend on their bacterial hosts for replication. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of 735 kb, which is-to our knowledge-the largest phage genome to be described to date. Thirty-five genomes were manually curated to completion (circular and no gaps). Expanded genetic repertoires include diverse and previously undescribed CRISPR-Cas systems, transfer RNAs (tRNAs), tRNA synthetases, tRNA-modification enzymes, translation-initiation and elongation factors, and ribosomal proteins. The CRISPR-Cas systems of phages have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. In addition, some phages may repurpose bacterial CRISPR-Cas systems to eliminate competing phages. We phylogenetically define the major clades of huge phages from human and other animal microbiomes, as well as from oceans, lakes, sediments, soils and the built environment. We conclude that the large gene inventories of huge phages reflect a conserved biological strategy, and that the phages are distributed across a broad bacterial host range and across Earth's ecosystems

    Integrating Entrepreneurship into the Design Classroom: Case Studies from the Developing World

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    © 2017, Springer Science+Business Media, LLC. Developing countries are more and more committed to building a knowledge-based economy as a means to diversify from their current resource-based economy. The current focus of many governments is on technology with real insights on creative economy and arts. In this context, universities are seen as a key partner of the government. This article presents the results of two innovative case studies of professors working in the College of Art and Design collaborating with a professor in the College of Business to integrate the concepts of entrepreneurship into their interior design courses. This was done through designing space for entrepreneurial projects and by the students acting as entrepreneurs themselves with an external client. This dual model of training combines (1) learning processes about the habits and the needs of entrepreneurs and (2) learning by acting as an entrepreneur. Such methods demonstrate the role of universities to provide a proper theoretical background for students and to foster entrepreneurial behaviors through arts entrepreneurship education. Furthermore, the central role of professors to introduce innovative teaching methods to combine entrepreneurship and the creative economy into non-business courses is an important finding in these case studies

    The effect of intraoral aging of the working stainless steel archwire on the rate of premolar extraction space closure: a randomized clinical trial

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    Objectives: The aims of this study were to investigate the effect of the working archwire intra-aging on the rate of upper first premolar space closure and to measure frictional resistance during space closure. Methods: A total of 28 subjects participated in this study. All patients were treated by upper first premolar extraction. Subjects were subdivided into 2 groups: non-replacement side: consisted of the right or left sides of the upper arch where space closure was done using the same 0.019 × 0.025-inch SS; replacement side: comprised the other side of the subjects where the working archwire was replaced with a new one each visit. The working archwire in the upper arch was split into 2 halves (new archwire on one side and old one on the other side); each one-half was connected to the other in the midline by a joining shim. Elastomeric chain was used to close extraction spaces. The amount of space closure (mm) was recorded each visit for 3 months. Results: In the non-replacement side, the rate of upper space closure was 0.80 mm/month coronally and 0.75 mm/month gingivally. In the replacement side, it was 0.69 mm/month coronally and 0.67 mm/month gingivally (p > 0.05). Mean frictional force for the non-replacement side was 3.58 ± 1.20 N, and it was 2.43 ± 1.21 N for the replacement side (p < 0.01). Conclusions: Intraoral archwire aging has no effect on the rate of upper premolar space closure/month although frictional resistance of the aged archwire was higher than of the new one. Clinical relevance No need to replace 0.019 × 0.025-inch SS every visit during space closure to overcome corrosion and frictional resistance.The study was supported by the Deanship of Research at Jordan University of Science and Technology (research grant number 53/2019)

    DNA interference states of the hypercompact CRISPR–CasΦ effector

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    CRISPR-CasΦ, a small RNA-guided enzyme found uniquely in bacteriophages, achieves programmable DNA cutting as well as genome editing. To investigate how the hypercompact enzyme recognizes and cleaves double-stranded DNA, we determined cryo-EM structures of CasΦ (Cas12j) in pre- and post-DNA-binding states. The structures reveal a streamlined protein architecture that tightly encircles the CRISPR RNA and DNA target to capture, unwind and cleave DNA. Comparison of the pre- and post-DNA-binding states reveals how the protein rearranges for DNA cleavage upon target recognition. On the basis of these structures, we created and tested mutant forms of CasΦ that cut DNA up to 20-fold faster relative to wild type, showing how this system may be naturally attenuated to improve the fidelity of DNA interference. The structural and mechanistic insights into how CasΦ binds and cleaves DNA should allow for protein engineering for both in vitro diagnostics and genome editing
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