Protein Patterns and Plasmid Profiles of the Bacterial Strains Isolated from a Poultry Slaughterhouse in Ankara, Turkey

A total of 65 identified isolates of coliform bacteria Salmonella, including Campylobacter and Staphylococcus isolated from different control points of a poultry slaughterhouse in Ankara, Turkey were characterized by morphological, biochemical and physiological tests including API 10 S system, and by plasmid profiles on agarose gel electrophoresis and whole-cell protein patterns on SDS-PAGE. Plasmids were detected in 53.8 % of the isolates. The molecular mass of the plasmids was within the range from 0.66 to 12.66 mDa. Electrophoretic banding patterns showed that whole cell protein profiles differed in several protein bands in Salmonella, Campylobacter and Staphylococcus species, but the differences were insufficient for reliable differentiation of bacteria species by SDS-PAGE method

Protein Patterns and Plasmid Profiles of the Bacterial Strains Isolated from a Poultry Slaughterhouse in Ankara, Turkey

A total of 65 identified isolates of coliform bacteria Salmonella, including Campylobacter and Staphylococcus isolated from different control points of a poultry slaughterhouse in Ankara, Turkey were characterized by morphological, biochemical and physiological tests including API 10 S system, and by plasmid profiles on agarose gel electrophoresis and whole-cell protein patterns on SDS-PAGE. Plasmids were detected in 53.8 % of the isolates. The molecular mass of the plasmids was within the range from 0.66 to 12.66 mDa. Electrophoretic banding patterns showed that whole cell protein profiles differed in several protein bands in Salmonella, Campylobacter and Staphylococcus species, but the differences were insufficient for reliable differentiation of bacteria species by SDS-PAGE method

Phosphorus-nitrogen compounds. Part 48. Syntheses of the phosphazenium salts containing 2-pyridyl pendant arm: Structural characterizations, thermal analysis, antimicrobial and cytotoxic activity studies

The phosphazenium salts (protic ionic liquids, PILs/protic molten salts, PMOSs) (6a-6d and 7a) of the free phosphazene bases (4a-4d and 5a) were prepared by the reactions of the corresponding cyclotriphosphazenes with the bulky gentisic acid. The structures of the PMOS were evaluated using the elemental analyses, FTIR, 1H, 13C{1H} and 31P{1H} NMR data. The molecular and crystal structures of 4a and 6c were established by X-ray crystallography. The thermal properties of the PMOS were determined using TG and DTA techniques. On the other hand, the antimicrobial activities of the free phosphazene bases (4a-4d and 5a-5d) and PMOSs (6a-6d and 7a) were screened against the selected bacteria and yeast strains. The antimicrobial activities of the free phosphazene bases and the PMOSs were compared. The interactions of the phosphazenes and their salts with plasmid DNA were elucidated by the agarose gel electrophoresis. The evaluations of the cytotoxic activities of these compounds were also studied against to L929 fibroblast and breast cancer cells (MDA-MB-231).  

Synthesis, (E)/(Z)-isomerization, and DNA binding, antibacterial, and antifungal activities of novel oximes and O-substituted oxime ethers

A series of novel positional oximes (2a-2d), O-methyl oxime ethers (3a-3d), and O-benzyl oxime ethers (4a-4d) were synthesized in high yields starting from their corresponding methyl 3-, 4-, 6-, and 13-keto tetradecanoates. The synthesized compounds were characterized by H-1 NMR, C-13 NMR, FT-IR, mass, and elemental analyses for their structures and (E) /(Z) isomerizations. Their DNA binding abilities were investigated in vitro by agarose gel electrophoresis. The antibacterial and antifungal activities were tested also in vitro against eleven bacterial strains and three fungal strains. The relationship between the structure and the mentioned biological activities was discussed. Compound 2a showed good antibacterial activity against five types of bacteria. Compounds 2b, 2c, 2d, and 4d were effective against several microorganisms. Among these, 2a showed the best DNA binding, antibacterial, and antifungal activities. Therefore, 2a can be a pro-drug as an anticancer, antibacterial, and antifungal agent

Numerical analysis of Candida species from uriner system infections based on SDS-PAGE and detection of antifungal resistance

Aim: The aim of the present study was to evaluate the protein patterns and numerical analysis of Candida species isolates from urinARY system infections by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and to detect antifungal susceptibility. Methods: In this study, 96 Candida spp. belonging to 46 strains of C.albicans, 18 strains of C. glabrata, 14 strains of C. tropicalis, 8 strains of C. krusei, 6 strains of C. parapsilosis, 3 strains of C. dubliensis and 1 strain of C. kefyr from urine samples or urinary system infections were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The susceptibility of Candida strains against antifungal agents were assessed by minimal inhibitory concentrations (MIC). Results: Nineteen distinct band patterns in the range of 63-120 kDa were obtained. Different isolates of each species were clearly different in each of the seven species. But, some variation in the protein patterns was detected within the same species. Conclusion: The whole-cell protein profiles performed by SDS-PAGE associated with computer-assisted numerical analysis may provide preliminary criteria for taxonomic and epidemiological studies of such microorganisms