Trespassers on Indian lands

50-1Indian AffairsTrespassers on Indian Lands. [2600] Legislation proposed.1888-10

Global Efforts to Address Severe Acute Malnutrition.

Detailed description on the two formats presented, proBAM (S1A) and proBed (S1B). (XLSX 46 kb

minor

Viola adunca Smithhooked violet;early blue violet;hooked-spur violet;sand violet;western dog violet;heath dog violetviolette à éperon crochuCrandell Mountain, S. slopedamp dirt & rock6800 feetviole

世代間交流で生まれるもの(第22回児童文化研究大会 研究報告)

application/pdfdepartmental bulletin pape

minor

Viola adunca Smithhooked violet;early blue violet;hooked-spur violet;sand violet;western dog violet;heath dog violetviolette à éperon crochuSect. of Livingstone Range, W of AGT Util. Stn (N Burmis Road)Moist shrubby patch sheltered in lee of E slope, below med el

Legislative, Executive, and Judicial Appropriations Bill

52-1AppropriationsLegislative, Executive, and Judicial Appropriations Bill. [2914] Year ending 30 June 1893; OIA.1892-21

TC-1 enhanced migration is associated with induction of EMT markers: TC-1 lysate was subjected to Western Blot using antibodies for Vimentin (57kDa) and Snail (29kDa).

<p>Histograms show the relative protein levels of each normalized to the intensity of the corresponding GAPDH values. In all cases, differences were considered significant at: * P<0.05; ** P<0.01; *** P<0.001.</p

Settlement of Cherokee Claims

49-1Indian AffairsSettlement of Cherokee Claims. [2360] Western, or "Old Settler" Cherokees; fulfillment of treaty stipulations.1886-16

A) Effect of Irradiation and culture with CM on TGF-β Expression in Wt fibroblasts.

<p>Whole cell lysate from Wt Fibroblasts was subjected to Western Blot using antibodies for TGF-β1 (13 kDa). Histogram shows relative protein levels normalized to the intensity of the corresponding GAPDH values. In all cases, differences were considered significant at: * P<0.05; ** P<0.01; *** P<0.001. <b>B) Modulation of TC-1 migration by SB 41542</b>. Migratory potential of TC-1 cells was analyzed and quantified in various culture conditions at 0 and 24 Hrs after wounding. Culture conditions are: CM from TC-1 non Irradiated (0Gy) and irradiated at 2, 10 Gy alone or cultured with CM from Wt fibroblasts with SB41542. Images are acquired at ×20 with Nikon Phase contrast, Japan. In all cases, differences were considered significant sat: * P<0.05; ** P<0.01; *** P<0.001.</p

Experimental Scheme.

<p><b>A) Step 1</b> Wt and RhoB-/- fibroblasts are cultured at subconfluence and irradiatedTC-1 are also cultured at subconfluency. <b>Step 2</b> After 24hrs, Conditioned media (CM) produced by the three type of cells irradiated or not are collected. CM from irradiated or Wt and RhoB -/- fibroblasts are used to culture TC-1 CM from TC-1 is used to culture Wt and RhoB-/- fibroblasts <b>Step 3</b> 24hrs later various experiments are performed. <b>B)</b> For simultaneous irradiation experiments. <b><i>Left panel</i> Step 1</b> TC-1 Cells are irradiated <b>Step 2</b> 24h later irradiation TC-1 medium is replaced by the CM produced by irradiated Wt or RhoB-/- fibroblasts <b>Step 3</b> 24 h later experiments are performed: <b><i>Right panel</i> Step 1</b> Wt and RhoB -/- fibroblasts are irradiated <b>Step 2</b> 24h later irradiation fibroblasts medium is replaced by the CM produced by irradiated TC-1 <b>Step 3</b> 24 h later experiments are performed.</p