Subcellular localization of serum and glucocorticoid inducible protein kinase(SGK) in renal cells and relationship between SGK and aldose reductase

血清和糖皮质激素诱导蛋白激酶（serumandglucocorticoidinducibleproteinkinase,SGK）是一种新发现的丝/苏氨酸蛋白激酶，与其他蛋白激酶主要在酶活性水平上受翻译后的磷酸化和去磷酸化调控显著不同的是，SGK1的转录、活性以及亚细胞定位受到不同胞内和胞外刺激因素的调节，是多种胞内信号途径的功能性交汇点，参与了细胞增殖、渗透调节、离子通道调节以及细胞生存和/或凋亡应答等过程，与糖尿病肾病、高血压等疾病密切相关。 然而，SGK1在信号转导通路中的分子机制还不是很清楚，其底物的发现也很有限。尤其SGK1的亚细胞定位研究仍存在很多争议，在细胞内表达外源的SGK1与...Serum and glucocorticoid inducible protein kinase, SGK is a novel member of the serine/threonine protein kinase gene family. Unlike the vast majority of protein kinases, which are predominantly regulated at the enzymatic level by posttranslational phosphorylation and dephosphorylation, the transcript expression, activity and subcellular localization of SGK1 can be acutely controlled by a diverse s...学位：理学硕士院系专业：生命科学学院生物学系_水生生物学学号：2005130200

细胞三维动态培养微器件的设计与制作

细胞培养是进行细胞研究的基础,为了在细胞体外培养时提供一种近似于体内的微环境,设计了一种可供细胞三维动态培养的微器件。首先设计了用于输运流体的微通道网络,培养池对称布置于微通道网络中,通过一系列\"多进多出\"型微通道分别与进样口和出样口相连。利用Comsol软件中的层流物理场和多孔介质物理场耦合对培养池内的流场进行仿真,通过比较流场的均一性和稳定性优化微通道网络结构。然后,采用静电直写技术在培养池内集成聚己内酯(PCL)三维支架,构建细胞三维培养空间。最后,封合微器件,检测微器件培养池内的流体流动情况,并进行细胞实验。实验结果表明,\"2×2\"型微器件培养池内的流体稳定性和均一性较好;PCL三维支架的纤维间距400μm,纤维直径80μm,孔隙率64%,细胞存活率达到90%以上。该细胞三维动态培养微器件更好地模拟了生物体内细胞生存所需的微环境,培养池内的细胞生长良好,满足设计要求。国家自然科学基金资助项目(No.51475079,No.51375076

基因编辑的技术分析与思考

基因编辑技术已成为当今非常重要的生物工程技术，在生物医药、生物环保以及农业生产领域有着极 其广泛的应用。本研究通过对基因编辑相关专利和期刊文献的分析，揭示了基因编辑技术的发展现状与趋 势，并基于对CRISPR技术的专利分析以及重要研发机构的专利布局分析，对我国基因编辑技术的发展提出了 对策与建议，为相关领域的研究人员及决策者提供参考。</p

四苯基铁卟啉双氧加合物的密度泛函理论研究

采用密度泛函理论方法计算了自旋多重度为1、3、5的四苯基铁卟啉双氧加合物(FeTPPO2)的几何构型及电子性质。考察了四苯基铁卟啉对分子氧的活化。研究发现,FeTPPO2能量随自旋多重度升高而降低。五重态FeTPPO2的能量最低,但其铁卟啉对分子氧的活化效果最差。FeTPPO2中Oa&mdash;Ob键键长随自旋多重度变化的规律为三重态&gt;单重态&gt;五重态,说明三重态FeTPPO2中分子氧活化最明显。Fe&mdash;Oa键键长和Oa&mdash;Ob键键长呈负相关关系。电荷布居分析表明,FeTPPO2中分子氧活化程度与分子氧上所带负电荷多少有关,所带负电荷越多,分子氧活化越明显。分析自旋密度得知,三重态FeTPPO2中分子氧为单线态分子氧。三重态和五重态FeTPPO2中,铁离子均向分子氧转移了&beta;电子,有利于铁氧键的形成

Histochemical Study of Polysaccharides and Lipids on the Developing Anthers of Impatiens balsamina

以不同发育时期的凤仙花花药为实验材料,采用组织化学方法,对花药发育中的结构变化及多糖和脂滴物质分布进行观察。结果表明:(1)凤仙花的花药壁由6层细胞组成,包括1层表皮细胞,2层药室内壁细胞,2层中层细胞和1层绒毡层细胞。其中绒毡层细胞的形态不明显,很难与造孢细胞区分,且在小孢子母细胞时期退化。(2)在小孢子母细胞中出现了一些淀粉粒,但减数分裂后,早期小孢子中的淀粉粒消失,又出现了一些小的脂滴;随着花粉的发育,小孢子形成大液泡,晚期小孢子中的脂滴也消失;小孢子分裂形成二胞花粉后,营养细胞中的大液泡降解、消失,二胞花粉中又开始积累淀粉;接近开花时,成熟花粉中充满细胞质,其中包含了较多的淀粉粒和脂滴。(3)在凤仙花的花药发育中,绒毡层细胞很早退化,为小孢子母细胞和四分体小孢子提供了营养物质;其后的中层细胞退化则为后期花粉发育提供了营养物质。The anther development of Impatiens balsamina displays some special characters:(1)Anther wall consists of 6 layers of cells:one epidermis,two endotheciums,two middle layers and one tapedum.Tapetal cells are not clear during sporogenous stage and difficult to distinguish with sporogenous cells.The tapetal cells degenerate during the stage of microspore mother cell(MMC).(2)Some starch polysaccharides appear in the microspore mother cells which had formed a callose wall.After meiosis of MMCs,starches in early microspores disappear,and lipids were accumulated in early microspores.With microspore developing,late microspores formed several large vacuoles and lipids disappeared.After microspore division,comparing with the big vacuoles in vegetative cell disappearing,starches again appeared in the 2-cellular pollen.Nearly anthesis,mature pollen grains of I.balsaminaaccumulated many lipids and starches as storey materials.(3) During anther development of I.balsamina,some cells of anther wall degenerated and their cytoplasm transformed lipids for pollen absorbing,the tapetal cells degenerated and offered lipids for MMCs and microspores,and middle layer cells for bicellular pollen grains.国家自然科学基金(31100225); 公益性行业(农业)科研专项课题(200903016); 浙江省自然科学基金(Y3110395

基因编辑的技术分析与思考

基因编辑技术已成为当今非常重要的生物工程技术，在生物医药、生物环保以及农业生产领域有着极 其广泛的应用。本研究通过对基因编辑相关专利和期刊文献的分析，揭示了基因编辑技术的发展现状与趋 势，并基于对CRISPR技术的专利分析以及重要研发机构的专利布局分析，对我国基因编辑技术的发展提出了 对策与建议，为相关领域的研究人员及决策者提供参考。</p

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies