单个纳米颗粒的光散射检测技术进展

纳米颗粒因其在生物医学和生物分析领域具有重要的应用前景而备受关注.单个纳米颗粒的光散射检测技术是一种简单、有效地对纳米颗粒的尺寸、尺寸分布及浓度等进行表征的分析方法,尤其在揭露纳米颗粒的内在异质性方面具有独特优势.然而瑞利散射强度随粒径减小呈六次方衰减,使得小尺寸单个纳米颗粒的检测非常具有挑战性.本文对近年发展起来的多种单个纳米颗粒的光散射检测技术进行综述

修形齿轮弯曲疲劳寿命对比试验研究

为探究修形参数对齿轮疲劳特性的影响,采用罗卡提法进行齿轮疲劳特性对比试验。试验采用错齿啮合方案,利用Romax确定了试验载荷相应的弯曲应力,并用Matlab对试验数据进行了处理分析,得到不同修形参数下的齿轮弯曲疲劳强度。试验结果表明,在给定工况下,选择合适的齿顶修形能够提高齿轮的弯曲疲劳寿命,为修形方法在齿轮中的应用提供了基础数据参考

EFFECTS OF THYMOSIN ON CONCENTRATIONS OF CYTOKINE IN SERUM OF PATIENTS WITH CHRONIC HEPATITIS B

目的观察大剂量国产胸腺肽对慢性活动性乙肝患者血清细胞因子的影响，探讨胸腺肽治疗慢性乙肝的机制。方法选择慢性活动性乙肝患者34例，分成两组：胸腺肽组19例，一般护肝组15例。比较观察治疗前后患者血清IFN—α、sIL－2r、TNF，和β2－MG为指标的变化。结果胸腺肽治疗后，患者PBMIFN—α诱生能力较治疗前上升119．99％，血清sIL—2r和TNF含量分别下降了35．56％和41．80％，治疗初期对β2—MG有提升作用。一般护肝组对慢性乙肝患者PBMIFN－α诱生能力和下调血清sIL-2r、TNF的作用弱于胸腺肽，对β2—MG指标没有影响。结论胸腺肽治疗慢性乙肝疗效与促进IFN产生和下调sIL-2r和TNF等多项免疫调节功能有关。Aim To dtudy the effects of high-dose thymosin on the cytokine conentration in serum of the patients with chronic hepatitis B and its therapy mechanism.Methods 34 patients with chronic active hepatitis B were divided into two groups; 19 patients were treated with thymosin and 15 patients with common drugs of protecting liver cells (PLC) .Level changes of IFN-α,sIL-2r, TNF, and β2-MG in the patients' serum were analyzed comparatively before and after treatment.Results After treating of thymosin, ability of induced-producing IFN-αin patients' PBMC was increased 119.99% over before treatment; concentrations of sIL-2r anu TNF in serum were decreased by 33.56% and 41.80%, respectively;and β2-MG in serum were increased in the early days of thymosin treatment.PLC showed similar, weaker effect on IFN-α, sII-2r, and TNF than that thymosin did; but no significant effects on β2-MG. Conclusions Mechanism of thymosin against HBV reproducing is relative to the the effets enhancing IFN-α producing abilities ,downing the serum levels of sIL-β2r and TNF,and so on.厦门市科委资助!Z9880

Characteristics of Vibrio alginolyticus Biofilm Formation

作者简介：姚刚(1983-)，男，硕士研究生；研究方向：环境微生物学.E-mail:[email protected] ,通讯作者：鄢庆枇(1963-),男, 教授；研究方向：水产病害．E-mail:[email protected][中文文摘]采用改良的微孔板法研究静置培养条件下致病性溶藻弧菌ND-01在酶标板上成膜情况。结果显示,溶藻弧菌的生物膜的OD590在16h达到峰值后,随培养时间延长出现下降;在102～108cfu/mL范围内,不同的初始菌密度对生物膜的OD590影响并不显著;NaCl质量分数为4.5%时,所形成生物膜的OD590最高;30℃,溶藻弧菌的生物膜OD590显著高于其他温度组(P<0.05);在胰蛋白胨大豆肉汤培养液中加入质量分数1.5%的CaCl2,能够显著促进生物膜形成,而加入MgCl2对于生物膜的影响则不显著;经过大黄鱼表皮黏液包被后,96孔板中生物膜的OD590显著高于其他部位组织提取液包被组(P<0.01)。致病性溶藻弧菌ND-01能够在聚苯乙烯材料的96孔酶标板表面形成稳定而明显的生物膜,且其生物膜的形成与多种环境因素有着密切关系。[英文文摘]A modified microtiter-plate test was introduced to determine biofilm formation of pathogenic Vibrio alginolyticus ND-01 on the surface of the polystyrene 96-well microtiter plate.Results showed that OD590 of V.alginolyticus strain ND-01 biofilm was found to peak after 16 h;within the 102~108cfu/mL,bacterial concentrations of V.alginolyticus exhibited no significantly effect on the biofilm formation;that the OD590 values of bacterial biofilm increased with the incubation temperatures of 4~30 ℃ and peaked at 30℃(P＜0.05); and that the OD590 values of bacterial biofilm fluctuated with different NaCl concentrations and optimized at 1% to 7%,the remarkablly higher OD590 values at pH 7-9,and peaked at pH7.Addition of 1.5% CaCl2 signficantly promoted the bacerial biofilm formation while MgCl2 played no significantly role:The OD590 of large yellow croaker the biofilm developed on the surface of the microtiter plate coated by the mucus layer on the skin of large yellow croaker was significant (P＜0.01).The results showed that V.alginolyticus strain ND-01 developed stable and evident biofilm,the biofilm formation was remarkably affected by environmental factors,such as temperature ,pH,NaCl concentration,and Ca2+ level.The results also showed that the bacterial biofilm formation of V.alginolyticus was governable,which would be useful for the epidemic control in fish culture.国家“863”计划项目(2007AA09Z115); 农业部公益性行业科研专项(200903029