Determination of Trace Ammonia in Water by Fluorescence Spectrophotometry Combining with Flow-Injection Analysis

研究了在碱性介质中， 基于氨（ 铵离子） 与邻苯二甲醛及2 - 巯基乙醇反应生成强荧光性吲哚取代衍生物的体系测定微量氨（ 铵离子） 的流动注射荧光分析法. 非离子表面活性剂曲拉通X-100 （TrITOn X- 100） 对该体系具有良好的增稳作用. 研究了该体系测定氨（ 铵离子） 的最佳实验条件. 测定氨（ 铵离子） 的线性范围0 ~0-7Mg/l， 相对标准偏差为1-3 % （ CnH3 ＝ 0-2Mg/l，n ＝ 11） ，检测限为0-0012Mg/l.A method is described for determination of trace ammonia by fluorescence spectrophotometer combining with flow-injection Analysis.The method is based on the system:in base media,ammonia reacts with o-phthaladehyde(OPA) and 2-mercaptoethanol(ME) producing derivative of indol which has intensive fluorescence.Nonionic surfactant Triton X-100 enhances stability in the system.The optimum reaction condition is investigated.A linear relationship between ammonia concentration and fluorescent intensity is observed over the range 0 1～0 7mg/L with a relative standard deviation(RSD) of 1 3% at ammonia concentration of 0 2 mg/L(n=11).The limit of determination is 0 001 2mg/L.国家863计划资助!（818－Q－09

Research of Oxygen Fiber-Optic Sensor with Complexes of Ru(Ⅱ) as Probe

研究了PH、温度对钌（ Ⅱ） 的配合物ru （bPy）3 Cl2 及ru （PHEn）3 Cl2（bPy ＝ 2 ，2′- 联吡啶，PHEn ＝ 1 ， 10 - 邻菲罗啉） 荧光性质的影响， 并试验了O2 对ru （PHEn）3Cl2 在水溶液、醋酸纤维素膜及多孔塑料光纤中的荧光猝灭情况.Determination of trace O 2 based on O 2 quenchingfluorescence of complexes of Ru(Ⅱ) has been an advanced research region in fiber-optic chemical sensor for recent years.Effects of pH,temperature on fluorescence of Ru(bpy) 3 Cl 2 and Ru(phen) 3 Cl 2 are described in this article.And O 2 quenching fluorescence of Ru(phen) 3 Cl 2 in water,celluloseacetate film,plastic porous fiber-optic is investigated too.国家863计划资助!（818－Q－09

Ammonia-Sensitive Porous Plastic Fiber Optical Probe Utilising Tri-iodo-fluorescein as Fluorescence Indicator

研究了以甲基丙烯酸甲酯、双甲基丙烯酸四乙二醇酯和庚烷为聚合原料， 藻红为荧光指示剂对氨敏感的多孔塑料光纤探头， 同时探讨PH 及氨对其荧光强度的影响.Ammonia-sensitive porous plastic fiber optical probe, which is polymerized by methyl methylacrylate,tetraethylene-glycol bis(methylacrylate) and heptane,utilising tri-iodo-fluorescein as fluorescent indicator is invistigated;effect of pH and ammonia is also discussed.国家863计划资助!（818－Q－09

DNA polymorphism difference between root system and rhizosphere soil arbuscular mycorrhizal fungi of Prunus mume by AFLP analysis

应用巢式PCr并进行梅根系与根围土壤丛枝菌根真菌(ArbuSCulAr MyCOrrIHIzAl fungI,AMf)dnA扩增片段长度多态性(AMPlIfIEd frAgMEnT lEngTH POlyMOrPHISM,AflP)的差异比较,研究梅共生AMf的作用机理。结果表明,从18个梅品种的30个根围土壤样品中有28个样品获得纯化的dnA片段,占样品数93.3%,样品平均多态性位点数为6.5个,nEI’S基因多样性为0.3559±0.1382,SHAnnOn信息指数为0.5299±0.1676。与梅根系AMf dnA多态性比较,根围土壤的平均多态性位点数明显较多;且根系AMf的dnA多态性位点绝大多数存在于土壤AMf的dnA多态性位点中,表明根系内AMf是由土壤AMf发育而来;根系与根围土壤AMf dnA的聚类均与梅品种群、品种关联性不强,表明AMf对宿主梅品种或品种群没有特异的共生关系。DNA polymorphism of arbuscular mycorrhizal fungi(AMF) in root system and those in rhizosphere soil of Prunus mume was analyzed through DNA amplification by nested PCR based on AFLP marker.The results show that the purified DNA can be extracted from 28 soil samples, accounting for 93.3% of totally 30 samples, collected from rhizosphere of 18 cultivars, averaging 6.5 loci for each sample.The average genetic identity was 0.3559±0.1382, Shannon information index was 0.5299±0.1676.The number of loci in rhizosphere soil was much more than that of loci in root sample, and most of loci in root sample could be found in soil sample.It was proved that the AMF in root might be developed from soil AMF.The clustered groups of AMF DNA by AFLP marker both from roots and from soils were not connected with cultivar groups or cultivars of P.mume, and indicated that there was no specific symbiotic relationship between AMF and P.mume.国家自然科学基金(30470006); 厦门市科技项目(3502Z20072010;3502Z20112004)~

Determination of Trace PO_4--(-3) by Quenching Fluorescence with Flow Injection Analysis

研究了以铝- 桑色素为荧光试剂， 在酸性条件下利用荧光熄灭法， 并结合了流动注射分析测定了水中微量磷酸根离子的方法. 测定PO3 -4 的线性范围1 ~9 Mg/l， 检测限为0-002 Mg/l， 1H可以测定60 份样品， 相对标准偏差为1-09 % .A method is investigated to determinate trace PO 4 3- in acid solution based on oxygen quenching aluminium-morin fluorescence intensity combining with flow-injection analysis.The linearship is between 1～9mg/L with the limit of detection 0 002mg/L.60 samples per hour can determinated by this method with RDS 1 09%.国家863计划资助!（818－Q－09

AFLP analysis of arbuscular mycorrhizal fungi in roots of Prunus mume

第一作者: 蔡邦平，博士，副研究员。主要研究方向: 园林植物与观赏园艺、植物菌根。电话: 0592--2039576 Email : cbangping@ 163.com 地址: 361003 福建省厦门市思明区虎园路25 号厦门市园林植物园。[中文文摘]为了解决梅根系共生的丛枝菌根(AM)真菌难以应用形态学鉴定的问题,以巢式PCR的AFLP方法研究梅根系AM真菌DNA多态性。试验采集梅花期的根系样品,应用改良CTAB法提取总DNA,经纯化处理后,应用巢式PCR扩增根系AM真菌基因片段,进行AFLP分析。结果表明,18个梅品种的30个根系样品中,仅有8个样品经巢式PCR后获得纯化的DNA片段,占试验样品数的26.7%;8个样品共得到指纹图谱带24条,各样品平均多态性位点数为3.0个,Nei’s基因多样性为0.4097±0.0848,Shannon信息指数为0.5968±0.0955;利用Nei’s遗传相似性系数聚类,梅品种根系内AMF基因组DNA的聚类类别与梅"品种群"这一分类级别无相关性。该试验为植物根系共生AM真菌DNA多态性研究提供了一种简便的技术。[英文文摘]DNA polymorphism of arbuscular mycorrhizal fungi (AMF) was analyzed through the method of DNA amplification by nested PCR based on AFLP marker,in order to solve the difficulty of identifying the species of AMF associated with mei flowers (Prunus mume Sieb.et Zucc.).A total of 30 root samples from 18 mei cultivars were collected in the flowering phase from Wuhan Mei Garden as experimental materials.The results show that the purified DNA can be extracted only from eight root samples that account for 26.7% of total root samples.Totally 24 polymorphic loci were obtained from eight sample roots，averaging 3. 0 loci for each sample.The average genetic identity was 0. 409 7 ±0. 084 8，and the Shannon information index was 0. 596 8 ± 0. 095 5． The clustered groups of AMF DNA by AFLP marker from different cultivars were not identical with cultivar groups of P.mume.The results indicate that the AFLP marker technology is a brief and effective method to study the DNA polymorphism for the AMF in the roots of a plant．教育部科学技术研究重点项目(104034);; 国家自然科学基金项目(30670047);; 厦门市科技计划项目(3502Z20072010、3502Z20112004

Study of pH Sensitive Membrane Based on Ion Pairs Technique

提出一种新型pH吸收传感膜。指示剂溴酚蓝与季铵盐阳离子以离子对的形式结合,均匀分布于增塑的PVC膜中。该传感膜测定pH的范围为4.0～6.0。对传感膜的制备方法及响应时间、可逆性、重现性等进行了研究。并应用于实际样品的测定,获得了比较好的结果。A new pH sensitive membrane based on ion pairs technique was developed. Ion pairs indicator, which was formed by bromo-phenol blue reacting with quaternary ammonium cetyl trimethyl ammonium (CTA) bromide, was homogeneously immobilized on plas-ticized PVC membrane. The sensitive membrane covers the pH range between 4.0 and 6.0. Preparation, response time, reversibility and reproducibility of the sensitive membrane were investigated. The sensitive membrane was used to determine the effluents in the mixture pool of antibiotic factory.教育部《高等学校骨干教师资助计划》项目;; 福建省自然科学基金(D9910009);; 福建省科技三项资助项目(K99037);; 福州大学科技发展基

Development on the Mass and Rapid Methods of Soil Testing and Plant Tissue Diagnosis

以3年的期程針對各地區大面積且具發展潛力之重點產業，研發及建立快速且大量化的土壤與植體營養診斷技術，結合各種作物體系肥料施用基準資訊，達成輔導農民準確施肥，生產優質農產品之目標。 The project will develop mass and rapid methods of soil testing and plant tissue diagnosis for the major crop sectors with large acreages and potential marketing. These methods will combine with many kinds of fertilizers application standards and information to guide the farmer produce good quality of agriculture products by accurate fertilizer application

空间实用背场Si太阳电池和GaAs/Ge太阳电池性能随质子辐照注量变化的比较

研究了空间实用背场Si太阳电池和GaAs/Ge太阳电池性能随质子辐照注量1 * 10~9 ～ 5 * 10~(13) cm~(-2)的变化。实验表明，两种太阳电池的电性能随辐照注量增加有不同的衰降趋势，背场Si太阳电池性能参数I_(sc)、V_(oc)和P_(max)衰降变化快，辐照注量为2 * 10~(10)cm~(-2)时，P_(max)就已衰降为原值的75％；而GaAs/Ge电池对应相同的衰降辐照注量达8 * 10~(11)cm~(-2), 且其I_(sc)、V_(oc)和P_(max)衰降变化起初缓慢，当辐照注量接近3 * 10~(12)~(-2)时才迅速下降。背场Si电池和GaAs/Ge电池性能衰降分别与擀子辐照引入的E_v + 0.14eV及E_v + 0.43eV和E_c - 0.41eV深能级有关

Study of pH sensitive membrane based on ion pairs technique

A new pH sensitive membrane based on ion pairs technique was developed. Ion pairs indicator, which was formed by bromophenol blue reacting with quaternary ammonium cetyl trimethyl ammonium (CTA) bromide, was homogeneously immobilized on plasticized PVC membrane. The sensitive membrane covers the pH range between 4.0 and 6.0. Preparation, response time, reversibility and reproducibility of the sensitive membrane were investigated. The sensitive membrane was used to determine the effluents in the mixture pool of antibiotic factory