STUDIES ON THE CHEMICAL CONSTITUENT FROM SEA CUCUMBER MENSAMARIA INTERCEDENS──Ⅱ.PMI-1, A NOVEL FUCOSAN

利用乙醇沉淀分级技术从二色桌片参双酶水解液里分离到一种纯的活性多糖，定名PMI-1。用高效薄层层析法鉴定其组成的单糖，发现PMI-1只含有L-岩藻糖。根据其分析结果和理化特性，PMI－1应是由若藻糖基和岩藻糖-4硫酸酯基组成的均多糖。研究PMI－1的核磁共振谱，可认定它是一种具有直链的和α1－α联接的均多糖。因此它是一种新的岩藻聚糖。Using double enzymes digestion and fractionations by precipitation with alcohol, a pure active polysaccharide PMI-1 was obtained from the dried body wall of Mensamaria intercedens. Monosaccharide composition was identified by thin-layer chromatography, the results indicate that PMI-1 only consisits of L-fucose. According to analytical and chemicophysical data, PMI-1 seemed to be homosaccharide consisted of fucose and fllcose-4sulfate with the molar ratio of 1: 1. Studied on the 1 HNMR and 13MNMR data of PMI-1 showed that PMI-1 had a straightsaccharide chain and α1-α linkage.福建省重中之重项

Regulation effect of exogenous nitric oxide on anti-oxidation system of Kandelia candel seedlings treated with NaCl

研究了外源一氧化氮(NO)供体硝普钠(SNP)对NaCl处理下红树植物秋茄(Kan-deliacandel)幼苗叶片中抗氧化酶活性、抗氧化物质及脯氨酸含量的影响。结果表明:NaCl处理下,秋茄幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等4种活性氧清除酶的活性均受到明显抑制(P<0.05),SNP可以不同程度地恢复SOD、POD、CAT的活性,但对APX活性影响不大;SNP提高谷胱甘肽(GSH)及类胡萝卜素(Car)的含量,促进脯氨酸含量的上升,显著降低叶片中过氧化氢(H2O2)和丙二醛(MDA)的累积。表明外源NO可以缓解NaCl处理诱导的秋茄幼苗叶片氧化损伤,降低膜脂过氧化水平,有利于秋茄适应盐生环境。By using sodium nitroprusside(SNP)as the donor of exogenous nitric oxide,this paper studied its effects on the antioxidant enzyme activities,anti-oxidation substances,and proline content in the leaves of Kandelia candel seedlings treated with NaCl.The results showed that after treated with NaCl,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)in K.candel seedling leaves were depressed significantly(P<0.05),while SNP could recover the activities of SOD,POD and CAT to different degrees but showed little effect on APX.SNP increased the contents of glutathione(GSH)and carotenoid(Car),promoted the production of proline,and decreased the accumulation of H2O2 and malondialdehyde(MDA)significantly.It was indicated that SNP could relieve the oxidation damages induced by NaCl via decreasing the level of membrane lipid peroxidation in K.candel leaves,and facilitate K.candel to adapt to saline environment.国家自然科学基金项目(30670317和39970438);; 厦门大学新世纪优秀人才支持计划项目(0000-X07115)资

Chemical Structure Study of Polysaccharide PCA1 Derived from Chlorella autotrophica

选取自养小球藻(Chlorella autotrophica)为研究对象,从中分离纯化得到3个组分,对其中的组分PCA1进行研究.电泳及高效凝胶过滤色谱法(HPGFC)鉴定其为均一多糖组分,熔点为226.9℃.相对分子量为1.3×105,气相色谱法及红外光谱显示其主要由α-D-吡喃葡萄糖组成,并含有一定量硫酸基.甲基化及13C-NMR结果表明PCA1是一种以1,4-的α-D-吡喃葡萄糖构成的均一多糖.Studies on algae have attracted more attention in these years due to the potential biological active materials.Recently,purified seaweed polysaccharides were found to have biological activities,such as antitumor,antivirus,antihyperlipidemia and anticoagulant.Chlorella autotrophica is a widely distributing alga that contains different polysaccharides in cell.Previous study on extract of chlorella autotrophica shows that it has antivirus ability to VHSV and ASFV.In order to test their bioactivities,three fractions of polysaccharide were isolated from Chlorella autotrophica and one of them,PCA1,was studied in this paper.Purity of PCA1 was determined with methods of electrophoresis and HPGFC,and the melting point is 226.9℃.The molecule weight of PCA1 is 134690,GC and FT-IR results show that it is mainly made of α-D-glucopyranoside and also functional group of sulfate radical.Both results of methylation and 13C-NMR suggest that PCA1 is most likely a α-(1→4) bonded glucopyranoside.国家自然科学基金(39870565);; 厦门大学科研基金(20005003)资

SiO_2-丙烯酸树脂复合涂层的电化学行为

为了增强丙烯酸树脂涂层的防腐蚀性能,采用硅烷偶联剂KH550分别对微米、纳米SiO_2粒子进行化学改性,再以丙烯酸树脂为基体材料,选择纳米、微米SiO_2填料并控制填料比例制成涂料,涂布在铸铁表面制成SiO_2-丙烯酸树脂复合涂层。采用傅里叶红外光谱仪(FT-IR)表征树脂固化程度,用电化学工作站测试涂层的电化学性能,以扫描电镜(SEM)观察加入纳米、微米SiO_2前后丙烯酸树脂涂层的断面形貌。结果表明:纳米和微米SiO_2的加入有助于提高丙烯酸树脂的耐腐蚀性能,尤其是纳米SiO_2,0.50%(质量分数)改性纳米SiO_2-丙烯酸树脂涂层耐蚀性最好,其Bode谱中低频区的阻抗值达到1.0×105Ω·cm2;改性微/纳米SiO_2复合改性同样可以提高丙烯酸树脂涂层的防腐蚀性能,涂层耐蚀性介于单组分改性纳米SiO_2涂层和改性微米SiO_2涂层之间,改性微、纳米SiO_2总用量0.50%,二者质量比1∶4时涂层抗腐蚀性能最佳

Effects of Exogenous Nitric Oxide on the Reactive Oxygen Species Metabolisms in Salt-treated Avicennia marina Seedlings

研究了在600 mmol.L-1NaC l处理下,外源一氧化氮供体SNP对白骨壤幼苗叶片中O2-.和H2O2水平、活性氧清除酶活性以及抗氧化小分子AsA、GSH和Car等含量的影响.结果表明:0.1 mmol.L-1SNP可以显著减少NaC l处理下白骨壤叶片中O2-.和H2O2的积累,增强SOD、POD和APX活性,但一定程度抑制CAT和LOX活性,促进AsA消耗,提高GSH和Car含量,从而缓解了NaC l处理下白骨壤叶片的活性氧胁迫.一氧化氮信号传递途径关键酶鸟苷酸环化酶抑制剂亚甲基蓝可以不同程度消除SNP对H2O2、CAT、LOX、POD以及抗氧化小分子AsA、GSH和Car的调节作用,但不能消除SNP对O2-.、SOD和APX的调节作用. 【英文摘要】 The producing rate of O2-·,contents of H2O2,AsA,GSH and Car,activities of the antioxidative enzymes involved in ROS scavenging invoked by sodium nitroprusside(SNP),an nitric oxide(NO) donor,were surveyed in Avicennia marina seedling leaves under 600 mmol·L-1 NaCl treatment.The results showed that,the producing rate of O2-· and content of H2O2 in A.marina leaves were dramatically reduced by 0.1 mmol·L-1 SNP under NaCl treatment.Meanwhile the activities of SOD,POD and APX were increased simultaneously,while t...国家自然科学基金(30271065,39970438);; 厦门大学新世纪优秀人才支持计划(0000-X07115)资

Studies on the Chemical Composition of Sea Cucumber Mensamaria Intercedens Ⅲ. Immunomodulative Effects of PMI-1

研究了二色桌片参多糖－１（ＰＭＩ－１）在体内、体外的免疫调节作用．结果表明，二色桌片参多糖－１体外能促进小鼠脾淋巴细胞增殖，促进小鼠脾淋巴细胞分泌ＩＬ－２；体内能显著促进迟发型超敏反应，增加脾指数、胸腺指数；具有显著的免疫增强作用。The immunoactivities of PMI-1 are studied in vivo and in vitro. PMI-1(5. 50. 100u g/mL ) in vitro could promote the proliferation of mice lymphocyte, and increase IL-2 production as well. In vivo, PMI-1 could significantly improve the delayed-type hypersensitivity (DTH) degree and the weights of spleen and thymus, although it has little effect on the production of hemolysin antibodies. The results indicate that PMI-1 could remarkedly enhance the immunofunction in mice

水溶性聚氨酯缓蚀剂及其制备方法与应用

本发明公开了一种水溶性聚氨酯缓蚀剂及其制备方法与应用。所述制备方法包括：以重量份数计，将10～30份聚乙二醇类化合物溶于有机溶剂中，并加入20～60份氰酸酯类化合物和1～3份催化剂于15～80℃混合反应，获得第一反应混合物；向所述第一反应混合物中加入10～30份苯胺低聚物，并于20～100℃反应，再经后处理获得所述水溶性聚氨酯缓蚀剂。本发明提供的新型水溶性聚氨酯缓蚀剂的制备工艺简单，原料廉价易得，同时所获的水溶性聚氨酯缓蚀剂使用方法简单，加水稀释即用，缓释时间长且稳定，安全环保，而且用量少，使用效果好，例如当该水溶性聚氨酯缓蚀剂的浓度为200mg-L时，对碳钢缓蚀率高达97.28％，适用于大规模应用