北京鸭、金定鸭及其杂种酯酶同工酶的研究

对北京鸭、金定鸭及其杂种大土北鸭酯酶同工酶的研究表明：同种鸭的胚胎与雏鸭及成鸭的酶谱存在较显著差别，表现了该同工酶的表达具有发育阶段特异性；在同样发育阶段同种鸭不同组织的酶谱也存在一定差异；这种组织特异性与组织的功能有密切关系；北京鸭与金定鸭酯酶同工酶表达在个体发育过程存在明显种质差异，这与二者不同经济性状特征的差异相一致；大土北鸭个体发育中酯酶同工酶表达接近于北京鸭，显示该杂种鸭酯酶同工酶性状较大程度继承北京鸭的遗传性福建省自然科学基

Universal primer PCR with SSCP and RFLP for identification of fish disease pathogens

采用通用引物PCR(UPPCR)、PCR RFLP、PCR SSCP技术 ,研究快速鉴别鱼病病原菌的分子生物学诊断技术。结果发现 ,采用细菌 16SrRNA基因保守区特异性引物 ,以嗜水气单胞菌、鲁克氏耶尔森菌、鳗弧菌、柱状曲挠杆菌、乙型链球菌、荧光假单胞菌等部分常见鱼病病原菌为对象 ,可以建立一种UPPCR技术。该技术能在保证实验条件不变的基础上 ,检出上述所有细菌 ,并还可检出大肠杆菌和双歧杆菌等非鱼病病原菌。并且认为 ,该法与SSCP配合即采用UPPCR SSCP技术能较好地鉴别被检菌而用于鱼病病原菌的快速诊断。A universal primer PCR(UPPCR), PCR RFLP and PCR SSCP had been screened for a molecular biological method that permitted the rapid identification of fish pathogens among them. The results showed that a universal primer PCR technology was available with specific primers from conserved regions of bacterial 16S ribosomal RNA genes. The bacteria tested included some common causative agents of fish diseases such as Aeromonas hydrophila, Yersinia ruckeri, Vibrio anguillarum, Flexibacter columnaris, beta Streptococcus, Pseudomonas fluorescens. The approach allowed the bacteria above and Escherichia coli and Bifidobacterium catenulatum detectable without any alterations of the experiment conditions. However, this study also found that the identification of species of bacteria tested depended on the combination of UPPCR and SSCP(PPCR SSCP), which was better than that of UPPCR and RFLP(UPPCR RFLP) and made rapid diagnosis of fish disease pathogens possible.国家自然科学基金!资助项目 (39770 5 85 );; IFS基金!(ImmunecomplexesinfreshfishinfectedFlexibactercolumnaris)资助项

菊芋块茎制高果糖浆的研究

通过菊芋干片与菊粉提取液的制备、菊粉酶解、精制生产高果糖浆，菊粉酶解的适宜条件为：底物糖浓度12%，加酶量26u/g糖，PH5.0-5.5，最适温度为50℃，酶解6H，底物降解率可达98.5%。菊粉酶解液经活性炭脱色、离子交换树脂处理、减压浓缩等步骤，制得糖浆的固形物含量为73.8%，果糖含量（占固形物）为83.6%，同时对糖浆的dE值、色度、灰分、微生物含量等指标进行了测定

Progress in Molecular Biological Studies of α_Tubulin in Maize (Zea mays)

自1963 年在植物细胞中发现微管以来，其研究取得了较大进展。α＿ 微管蛋白是组成微管的基本单位之一。本文综述了玉米α＿微管蛋白基因及其表达调控的研究进展The studies on microtubule have been made great progresses since microtubule was found in plant cells in 1963.Researches on the genes and the regulation of gene expression of α_tubulin in maize, a subunit of tubulin which is a major constituent of microtubule, are reviewed

乙肝病毒表面抗原基因在花生中的遗传转化及免疫原性检测

首次用花生半胚作为外植体,与农杆菌EHA105(含质粒p1301HBs)共培养5d,再生培养基中通过加入潮霉素进行抗性筛选,得到抗潮霉素的芽,经芽的伸长、诱导生根获得转化植株。经PCR、PCR-Southern杂交、Southern点杂交等分子检测,证实目的基因已整合到花生基因组中,ELISA检测证实了在花生中表达的HBsAg具有较好的活性。经初步定量,花生小芽的蛋白初提液中可溶性蛋白含量1.044g/L,HBsAg小蛋白的含量约占总可溶性蛋白的0.032%,每克转化植株小芽鲜重含HBsAg小蛋白约2.4×10-7g。转基因花生植株初提重组蛋白经HPLC纯化、浓缩后,注射初免一次的小鼠,有明显的特异性抗体产生。口服饲喂已免疫但抗体下降至0.025(HBsAbELISAD值)Balb/c小鼠,发现有较强的抗体回升,达3.54,表明转基因花生疫苗可以加强口服免疫

Mitochodrial DNA Restriction Maps of Gallus gallus domesticus Hetian Breed

运用差速离心法、蛋白酶k及rnASE消化法制备并纯化河田鸡（gAluSgAluSdOMESTICuSHETIAnbrEEd）的线粒体dnA（MTdnA）.用11种限制酶对其进行单酶切分析，结果表明，AVAI、bAMHI、bglI、drAI、ECOrI、HPAI、PVuⅡ、SACI、SAlI和STuI在河田鸡MTdnA上分别有5、2、4、5、1、3、4、3、3和＞9个酶切位点，SCAl在不同个体河田鸡MTdnA上检测出两种限制性格局，分别有2和3个酶切位点.此外，还用bAMHI、bglI、drAI、ECOrI、HPAI、PVuⅡ、SACI、SAlI和SCAI进行双酶切，构建了MTdnA的物理图谱Mitochondrial DNA (mtDNA) from Gallus gallus domesticus Hetian breed Francolinus was purified, and then digested with 11 restriction endonucleases(Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu Ⅱ, Sac I, Sal I, Sca I, Stu I).The results showed that 10 restriction types(Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu Ⅱ, Sac I, Sal I, Stu I)in different individuls were identical,and the number of restriction sites of the above enzymes was 5,2,4,5,1,3,4,3,3,>9 respectively.Only Sca I had difference,and some chicken had 3 restriction sites,and some had 2 restriction sites.Furthermore,restriction maps of mtDNA were obtained with the double digestion method.福建省自然科学基

Mitochondrial DNA Restriction Maps of Acryllium vulturinum

应用AvaⅠ、BamHⅠ、BglⅠ、DraⅠ、EcoRⅠ、HpaⅠ、PvuⅡ、SacⅠ、SalⅠ、ScaⅠ和StuⅠ共 11种限制酶对珠鸡(Acrylliumvulturinum)mtDNA进行单酶切分析 ,结果表明 ,珠鸡mtDNA的分子量为 16 .7kb ;另以其中除AvaⅠ和StuⅠ外的 9种限制酶进行双酶切分析 ,构建了珠鸡mtDNA的限制性图谱 ,并与家鸡 (Gallusgallusdomesticus)比较 ,二者mtDNA序列歧异值为 0 .0 73.Mitochondrial DNA (mtDNA) from Acryllium vulturinum was purified, and then digested with 11 restriction endonucleases ( Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu II, Sac I, Sal I, Sca I, Stu I ). The results showed that its molecular weight was 16.7 kb.From the comparison of mtDNA between Acryllium vulturinum and Gallus gallus domesticus,the sequence divergence value was 0.073. Furthermore, restriction maps of the mtDNA were obtained with the double digestion method

关于半番鸭及其亲本羽毛的微量金属组分的比较分析

半番鸭以金定鸭(Anas Platyrhyncha domestica)为母本、番鸭(Cairina mos-chata domestica)为父本的杂交子代。本研究着重对半番鸭及其亲本的羽毛进行有关微量金属元素组成分析,并分别测定其中对构成蛋白质和其他生命物质特别重要的铜、锌、铁的含量,通过比较彼此间的差异来探讨半番鸭与其亲本的遗传关系。样品分别取自黑羽型雄番鸭、雌金定鸭及雄半番鸭的飞羽(取样部位三种鸭完全一样),每种鸭均采样40只。金定鸭由厦门大学生物学系金定鸭实验站提供,番鸭和半番鸭由龙海县紫泥乡金定村养鸭场繁育。实验前将预先以蒸馏水洗过并干燥的羽毛浸泡于无水乙醚中三遍,除去油脂杂质,再以去离子水洗涤数次,置30℃烘箱干燥,用手术