应用AvaⅠ、BamHⅠ、BglⅠ、DraⅠ、EcoRⅠ、HpaⅠ、PvuⅡ、SacⅠ、SalⅠ、ScaⅠ和StuⅠ共 11种限制酶对珠鸡(Acrylliumvulturinum)mtDNA进行单酶切分析 ,结果表明 ,珠鸡mtDNA的分子量为 16 .7kb ;另以其中除AvaⅠ和StuⅠ外的 9种限制酶进行双酶切分析 ,构建了珠鸡mtDNA的限制性图谱 ,并与家鸡 (Gallusgallusdomesticus)比较 ,二者mtDNA序列歧异值为 0 .0 73.Mitochondrial DNA (mtDNA) from Acryllium vulturinum was purified, and then digested with 11 restriction endonucleases ( Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu II, Sac I, Sal I, Sca I, Stu I ). The results showed that its molecular weight was 16.7 kb.From the comparison of mtDNA between Acryllium vulturinum and Gallus gallus domesticus,the sequence divergence value was 0.073. Furthermore, restriction maps of the mtDNA were obtained with the double digestion method
