运用差速离心法、蛋白酶k及rnASE消化法制备并纯化河田鸡（gAluSgAluSdOMESTICuSHETIAnbrEEd）的线粒体dnA（MTdnA）.用11种限制酶对其进行单酶切分析，结果表明，AVAI、bAMHI、bglI、drAI、ECOrI、HPAI、PVuⅡ、SACI、SAlI和STuI在河田鸡MTdnA上分别有5、2、4、5、1、3、4、3、3和＞9个酶切位点，SCAl在不同个体河田鸡MTdnA上检测出两种限制性格局，分别有2和3个酶切位点.此外，还用bAMHI、bglI、drAI、ECOrI、HPAI、PVuⅡ、SACI、SAlI和SCAI进行双酶切，构建了MTdnA的物理图谱Mitochondrial DNA (mtDNA) from Gallus gallus domesticus Hetian breed Francolinus was purified, and then digested with 11 restriction endonucleases(Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu Ⅱ, Sac I, Sal I, Sca I, Stu I).The results showed that 10 restriction types(Ava I, BamH I, Bgl I, Dra I, EcoR I, Hpa I, Pvu Ⅱ, Sac I, Sal I, Stu I)in different individuls were identical,and the number of restriction sites of the above enzymes was 5,2,4,5,1,3,4,3,3,>9 respectively.Only Sca I had difference,and some chicken had 3 restriction sites,and some had 2 restriction sites.Furthermore,restriction maps of mtDNA were obtained with the double digestion method.福建省自然科学基
