Expression of Modified H5N1 Avian Influenza Virus M2 Gene and the Recombinant M2 Protein Immunogenicity Assay

禽流感的发生不仅会造成禽类的大量死亡,而且也严重地威胁着人类健康,接种疫苗是控制禽流感发生的主要措施之一.M2基因的保守性使其成为基因工程亚单位疫苗的目标抗原.本研究将M2基因的跨膜区删除后,构建原核表达载体PET32A-△M 2,IPTg诱导后,收获的细菌总蛋白SdS-PAgE电泳结果表明修饰的M2基因在原核表达系统中得到高效表达,表达蛋白以可溶性形式存在,WESTErn杂交和动物免疫结果表明重组蛋白具有抗原性和免疫原性.本研究结果为利用M2重组蛋白开发具有交叉保护作用的禽流感疫苗奠定了基础.The present vaccination is one of main strategy for control of avian influenza virus that critically threatened human health.Recent investigations of subunit component vaccine focused on M2 gene because M2 gene possessed a highly structurally conserved property among influenza viral strain.In this study,the recombinants prokaryotic expression plasmid pET32a-△ M2 harboring the deletion of the transmembrane segment of M2 gene was constructed.SDS-PAGE electrophoresis showed that modified M2 gene was highly expressed as fusion protein in a soluble form after genetic modified E.coli BL21(DE3) bacteria were induced with IPTG.The fusion protein of M2 possessed antigenicity and immunogenicity properties by Western Blotting analysis of fusion protein with standard antibody against M2(H5N1) and mouse vaccination of purified fusion protein respectively.The results laid a foundation of further study on the development of cross-protection avian influenza vaccine.福建省科技厅重点项目(2009N0051);福建省教育厅A类科技项目(JA09168

Study on Tomato Transformation with Human Keratinocyte Growth Factor-1

人角质细胞生长因子在组织的损伤修复中起着重要的作用.以番茄子叶为外植体,通过根癌农杆菌介导法,将人kgf-1导入番茄中,共获得12株独立的抗性转化植株,PCr和dnA斑点杂交结果表明:kgf-1基因整合入番茄基因组中,为获得植物源表达的kgf-1蛋白打下了基础.Keratinocyte growth factor plays an important role in the tissue damage repair.Using sterile tomato cotyledons as explants,the human KGF-1 gene had been introduced into the tomato genome by Agrobacterium-mediated transformation method.As a result,a total of 12 independent putative transgenic tomato plantlets were obtained.The results of PCR and DNA dot-blot hybridization showed that KGF-1 gene was integrated into the tomato genome.This work has laid a foundation for further studies on the development of KGF-1 proteins expressed in tomatoes.福建省自然科学基金(2010J01240

Progress on Gene Transformation of Cucumber

综述了黄瓜转基因研究所取得的进展,从黄瓜再生体系的建立,遗传转化方法及其影响因素等方面进行了评述,并对黄瓜转基因的不足及今后研究的重点进行了讨论.Cucumber(Cucumis sativus L.) has been extensively investigated for genetic engineering.Cucumber regeneration systems,genetic transformation methods and factors influencing transformation have been reviewed in this paper.Meanwhile,the problems and the research focus of cucumber transformation were discussed.漳州师范学院院内项目(SK05010)资

Studies on the Technical System of AntherCulture of Brassica oleracea var. acephala

本实验以6份羽衣甘蓝为材料进行花药培养.细胞学观察表明,当花蕾长为2.5-3.5mm,花瓣与花药长度之比为1/2~4/5时,45-65%的小孢子发育处于单核晚期,是花药培养的最佳时期.花药培养结果可见基因型不同,胚状体的诱导率有极大的差异,最容易形成胚状体的材料是Y3,诱导效率达17.74%;胚状体的出现在花药接种后的15-30天之间.培养基的种类不同,培养效果也有差异,其中以Keller培养基效果最佳;培养基中有机成份加倍,则可以显著提高胚状体的诱导效率.培养基中蔗糖的浓度对胚状体的诱导率也有显著的影响.Six cultivars of Brassica oleracea var. acephala were used in the study anther culture. The main results are as follows: The cytological observations of pollen showed that most of the pollen were in the late uninucleate stage when the ratio of petal to anther length was between 1/2~4/5 in Brassica oleracea var. acephala ,and the stage of late uninucleate was optimal for anther culture. The effects of genotype and cultural medium on embryoid induction were investigated in s Brassica oleracea var. acephala. The results showed it was optimal to induce the embryoid in Keller medium with double organic ingredients and 10% sucrose. The capacity of embryoid production varied significantly in different genotypes, and genotype Y3 was an ideal material for embryoid induction

风电齿轮箱行星齿轮副侧隙偏差原因分析

行星传动机构作为风电齿轮箱关键部件，因承受无规律的风力作用及强阵风冲击变载荷作用，其工作时的可靠性决定着整台风电齿轮箱传动的可靠性。作为齿轮传动4项要求之一——适当的齿侧间隙是齿轮副正常工作的必要条件，是降低行星轮系传动时冲击、噪声、空程误差，以及提高风电齿轮箱使用寿命的关键。针对一级行星传动结构形式的风电齿轮箱行星齿轮副侧隙偏大问题，从零件制造、装配、测量3个方面对行星轮副侧隙的影响进行了深入分析，确认了装配工艺对行星齿轮副侧隙的影响，通过选配中箱体与主轴承，解决了行星轮齿轮副侧隙偏差问题

基于波分复用技术的LED可见光通信的研究

介绍了LED（发光二极管）可见光通信系统和波分复用技术的原理,利用Optisystem软件设计了波分复用技术在可见光通信链路传输中的系统结构,实现了光路百兆级的实时通信。采用直接调制方式,对NRZ（非归零）码和RZ（归零）码调制方式下的传输性能进行了对比研究,分析了不同速率对系统的误码率和Q因子等因素的影响,进而讨论了在不同传输距离下系统误码率的变化特性,验证了波分复用技术应用在LED可见光通信系统中的可行性和稳定性

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies