Protection design and study of war relics in south Fujian Province

记忆的城市是乏味的，记忆与祈盼筑成生命的长河，时代前行的步伐可以挥去人类所经历的苦难，却挥不去积淀在后人心中对历史、文化和情感的渴望。如今，保护历史、珍存历史已经成为全人类的共识。 记载历史的方式有很多，文字、图画、艺术品等，而其中，战争遗址作为历史的延续，承载着大量的记忆和情感信息。许多与战争遗址相关的纪念性建筑、纪念性主题公园以其特有的建筑语汇与景观语言注释着人类文明史实，并因其所蕴含的丰富历史价值和情感内涵而成为人们情感寄托的象征物。其围绕着遗址本身进行保护设计所塑造的独具个性的形象，也起着无可替代的情感交流的媒介作用。纵观国内外有许多建筑大师在战争遗址保护设计领没有域颇多建树，创造出...A city with no memory is dull. Memories and expectations build the river of life. The memories of hardships may fade away with the pace of time, but the worship of history, culture and love will accumulate in people's heart. Today, the protection of history has become a common sense worldwide. There are many ways to record history, such as words, pictures and art, etc., of which, the war relics a...学位：建筑学硕士院系专业：建筑与土木工程学院建筑系_建筑设计及其理论学号：2522007115134

Research on the Application of the Profit Split Method to Transfer Pricing

本文旨在对利润分割法在转让定价中的应用进行研究。近年来，交易净利润法成为国内应用最为广泛的转让定价方法，然而随着我国产业结构升级、经济增长方式转变，以及跨国企业集团对在华外资企业功能风险定位的调整，交易净利润法因其本身的局限性，再加上作为发展中国家，该方法在具体运用中存在诸多挑战的现状，已越来越不能被认定为“最合适的转让定价方法”。基于对利润分割法与交易净利润法在特点及适用性方面的比较分析，本文指出，在现有国际与国内转让定价规范框架下，利润分割法可能成为解决复杂转让定价问题最合适的方法。 利润分割法在具体运用中需要解决合并利润、利润分割基准等基本问题，本文通过理论结合案例形式对上述问题进行了...The purpose of this article is to research on the application of the profit split method (PSM) to transfer pricing. Recently, the transactional net profit method (TNPM) has become the most widely-used method of transfer pricing in China. The TNPM, however, has its own limitations. Furthermore, along with the upgrade of industrial structure of China, the transformation of its economic growth, and t...学位：会计硕士院系专业：厦门大学与厦门国家会计学院会计硕士专业学位联合教育中心（MPACC）_会计硕士(MPACC)学号：X200515700

Coming from history——"Protection of Inheritance"——Block morphological analysis and transformation of the future in Longtou road of Gulang island in Xiamen

以厦门鼓浪屿龙头路的改造设计为例讨论历史街区保护开发。历史街区保护开发要与城市区域总体发展策划相适应,可以导入全新的区域开发、旅游开发理念,为区域的发展寻求一条独特的发展之道。在街区设计中挖掘、整合、升华、提炼历史文化内涵和特色,强化区域独特个性。挖掘改造设计的文化内涵,保持自身的文化特色和文化优势。对鼓浪屿龙头路进行品牌设计,有利于突出厦门鼓浪屿独特的社会文化环境,成为具有较高知名度、富有竞争力的城市区域。Take the reformation and redesign of the Longtou road in Gulang island of Xiamen as an example, discussing the protection and development of the historical street area.The historical street area protection and development should be adapt to the macroscopic developing plan of the city, it can contain the new principle of district development and tour development, looking for a special way for more progress.In the street area designing, scoop out, integrate, sublimate and refine the content and the features of the historical culture, enhance the character of different districts

Construction of polybutycyanocrylate nanoparticles(PBCA-NPs) loaded P16a gene and expression in Hep-2 cell

目的制备携带P16A基因的聚氰基丙烯酸正丁酯纳米粒并在喉癌细胞中进行表达。方法选用乳化聚合法制备PbCA-nPS,以激光粒度分析仪及透射电子显微镜分析纳米粒的形态和粒径。用阳离子表面活性剂十六烷基三乙基溴化铵(CTAb)对纳米粒进行表面修饰。构建真核表达载体PIrES2-EgfP-P16A经过酶切鉴定及测序后与PbCA-nPS连接,转染喉癌细胞HEP-2,荧光显微镜检测转染效率,蛋白印迹法检验P16A基因的表达,流式细胞术检测细胞凋亡率。结果所制PbCA-nPS粒径均匀、zETA电位较高,较为理想;重组质粒PIrES2-EgfP-P16A经过酶切鉴定及测序后,表明真核表达载体构建正确;PbCA-nPS可以介导PIrES2-EgfP-P16A高效转染喉癌细胞,蛋白印迹检测表明转染后喉癌细胞能够表达外源P16A基因,在其介导下P16A能有效抑制喉癌细胞的增殖并能诱导细胞凋亡。结论聚氰基丙烯酸正丁酯纳米粒可以作为一种良好的基因载体,为喉癌的基因治疗提供了新思路。[Objective] To prepare polybutycyanocrylate nanoparticles(PBCA-NPs)loaded P16a gene as the gene delivery system,and to express the P16a protein in Hep-2 cell line.[Methods] PBCA-NPs were prepared by the emulsion polymerization method.Surface of PBCA-NPs was surveyed by transmission electron micrographs(TEM),the grain distribution and zeta potentials of PBCA-NPs were determined with the laser grain analyzer.The PBCA-NPs surface was modified by the cationic surfactant cetyltrimethylammonium bromide(CTAB).Construct the eukaryotic expression vector pIRES2-EGFP-P16a and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing,the constructed eukaryotic expression plasmid was transfected to Hep-2 line.Transfect ion efficiency was observed by fluorescence microscope and the expression of P16a gene was detected by Western blot.Apoptosis was detected by flow cytomery.[Results] Nps with even size and smooth surface were successfully obtained,holding the higher zeta electric potential.The new constructed vector was confirmed by restricted enzyme and sequencing.pIRES2-EGFP-P16 can be mediated by PBCA-NPs with high transfection efficiency.Exogenous P16a gene can be expressed in transfected Hep-2 cell line detected by Western blot.P16a mediated by Nps can effectively inhibit the proliferation of Hep-2 cell,and induce Hep-2 cell apoptosis in vitro.[Conclusions] PBCA-NPs could be a good vector and provided a new way for gene therapy of laryngocarcinoma.厦门市卫生局科研立项专项资金(序号wsk09)资

Blind caption watermarking scheme based on ICA and DCT

标注水印为数字多媒体的便捷使用提供了一种新的途径。提出了1种结合独立分量分析(ICA)和离散余弦变换(dCT)的数字图像标注水印盲提取算法。该算法先将载体图像重采样成2个极为相似的子图,再分别将其分解成互不重叠的8x8子块,并进行离散余弦变换,接着将经过置乱后的水印嵌入到余弦变换的中频系数上,最后进行逆余弦变换得到嵌有水印的图像。采用ICA技术进行水印提取。实验表明,该算法有效可行。Caption watermarking is a new convenient way to use the digital multimedia.A blind scheme of digital image caption watermarking based on the independent component analysis(ICA) and discrete cosine transform(DCT) is proposed.Firstly,the cover image is sampled into two very similar sub-images.Secondly,the sub-images are decomposed into non-overlapping 8×8 blocks.Thirdly,these blocks are transformed into discrete cosine domain.Then the watermark is embedded into its middle frequency coefficient which has been rearranged by Scramble before embedded.Finally,we get image which is embed watermark by IDCT.It use the technique of ICA when the watermark is extracted.The results of emulation indicate this algorithm is available and feasible.福建省自然科学基金(2009J01301)资

Adaptive Blind Watermarking Algorithm Based on ICA and DWT

为了使数字水印具有较强的鲁棒性和不可见性,提出一种基于独立分量分析(ICA)和离散小波变换(dWT)的自适应鲁棒性盲水印算法.该算法先将载体图像进行小波变换,然后将原始水印进行平铺和置乱,再根据噪声可见函数(nVf)的视觉掩蔽作用,将其自适应地嵌入到载体图像小波变换域的低频系数上,最后利用ICA技术实现了水印盲提取.实验结果表明,该算法的水印不可见性较好,且可抵抗多种常见水印攻击,其中对JPEg压缩、尺度缩放等攻击的鲁棒性较突出,可应用于图像的版权保护.In order to enhance the robustness and invisibility of digital watermarking,a new adaptive watermarking algorithm based on independent component analysis(ICA) and discrete wavelet transform(DWT) is proposed.The algorithm firstly decomposes the image into wavelet domain.Then the watermark is adaptive embedded into its low frequency coefficient,under the visual mask of noise visibility function(NVF),which has been rearranged by tiling extended and scramble before embedded.It realizes blind extraction by ICA.Experimental results show that the algorithm has good transparency and better robustness,especially in JPEG compression and resizing scale attacks.It can be applicable for digital image copyright protection.福建省自然科学基金项目(2010J01210

理论与计算化学研究进展

化学是一门有坚实理论体系的基础学科,作为其中一个分支,理论与计算化学的飞速发展使得化学成为实验与理论并重的科学.结合价键理论方法、生物体系理论模拟和激发态光化学、固体表面与纳米结构体系、复杂体系分子量子动力学和密度泛函理论方法发展和核磁共振理论研究等,简要回顾了近期本研究团队和国内外本学科的发展情况,重点介绍了厦门大学在量子化学理论计算方法发展、计算程序研发、复杂体系的动力学以及酶催化反应的计算模拟等方面的研究

单管实时PCR快速检验ABO基因型

目的建立快捷特异的ABO基因分型检测方法。方法根据ABO基因结构特点,设计特异性引物和四色双链探针,采用单管实时PCR方法检测ABO基因,结果与传统免疫学方法相对比。结果该方法可检出常见的3个等位基因,区分常见的6种基因型,全部检测过程可在100min内完成。110例中国人的随机个体定型结果与传统免疫学方法一致。结论实时PCR法进行ABO基因分型,简便快捷,灵敏度高,可以有效地为侦查破案服务

植物挥发性气体(VOCs)研究进展

植物挥发性气体(VOCs)在植物-植食性昆虫-天敌三级营养关系、植物间信息传递及适应性改变上都发挥着重要作用。植物释放VOCs具特异性、系统性、时序性与节律性等特点,VOCs主要在寄主选择行为、产卵行为、求偶行为、引来昆虫天敌干涉等方面影响植食性昆虫。VOCs介导的植物间信息传递作用包括4个过程:"释放者"植物合成及释放气体、气体在空气中的运输、气体在植物表面的吸附及"接收者"植株对气体信号的感知。收集VOCs的方法主要有吸附-溶剂洗脱法和吸附-热脱附法

Synthesis and Bioactivity of 5-Ivermectin B_(1a) Esters

依维菌素B1a与羧酸在DMAP/DCC体系中直接酯化 ,得到 10个 5 IVB1a酯衍生物 ,产率 6 6 %～ 82 %;它们的化学结构得到IR ,1HNMR ,13 CNMR和MS谱的确证 .它们具有良好的杀虫、杀螨活性 .Ten 5-Ivermectin B 1a ester derivatives were prepared from Ivermectin B 1a and carboxylic acids in the systems of DCC/DMAP. Their chemical structures were determined by IR, 1H NMR, 13C NMR and MS spectra. The preliminary results of bioassay showed that L-1～L-10 have good insecticidal activities.国家自然科学基金(No .2 9832 0 5 0 )资助项