东方管理思想适用性探析

东方管理学是以中华优秀传统管理文化为核心所创造的。它不仅造就已维持达两千多年之久的中华统一国家,而且对于现在,东方管理学作为一种高境界的管理智慧,对于改善组织的管理状况,提升组织的管理水平,也具有重要的实践意义

杆状病毒用于哺乳动物细胞快速高效表达外源基因的研究

现已发现杆状病毒可进入某些培养的哺乳动物细胞 ,这提示可将杆状病毒作为一种对哺乳动物细胞的新型基因转移载体。对杆状病毒转移载体的改造及对哺乳动物细胞的基因转移方式进行了进一步的研究。以绿色荧光蛋白基因为报告基因 ,利用Bac to Bac系统构建了分别含有正向和反向CMV启动子表达盒的两种重组杆状病毒。可观察到CMV启动子在Sf9细胞中可启动报告基因的表达 ,但表达效率较低。用重组杆状病毒感染后Sf9细胞的培养上清直接与HepG2细胞作用 ,以流式细胞术检测基因转移效率及荧光表达强度 ,发现这两种病毒在相同的感染复数下对HepG2细胞具有相似的基因转移及表达效率。同时 ,利用流式细胞术进一步研究了直接使用重组杆状病毒感染 4d后Sf9细胞的培养上清对哺乳动物细胞进行基因转移的方法。通过对HepG2细胞的实验结果显示 ,将带毒Sf9细胞培养上清 (1.2× 10 7PFU mL)用哺乳动物细胞培养基 1倍稀释后 ,37℃下孵育靶细胞 12h(moi=5 0 ) ,可达到较高的基因转移及表达效率 ,同时不会对细胞造成明显损伤。将重组杆状病毒与脂质体和逆转录病毒这两种系统对HepG2及CV1细胞的基因转移效率进行了比较 ,结果发现在同样未经浓缩等特殊处理的条件下重组杆状病毒对这两种细胞的基因转移效率是最高的。因此可以认为

Experimental Study on Soil Infiltration Characteristics Using Disc Permeameter

介绍了一种田间定量测定土壤渗透性的新方法——圆盘入渗仪法。应用该法和双环法对黄土丘陵区土壤不同利用方式 (果园、农地、灌木、草地、林地 )下的土壤渗透性进行了对比研究 ,并对比分析了该方法的优缺点。结果表明 :在该试验条件下 ,不同利用方式下土壤的孔性及渗透性有明显差别。土壤有效孔径以灌木地最大 ,为农地的 5 .7倍 ,果园的 3.5倍 ;其次为林地和草地 ,为农地的 4倍多 ,果园的 2 .5倍多。果园的最初入渗率最大 ,是林地的3倍 ,草地的 5倍。稳定入渗率大小顺序为 :果园 >农地 >灌木 >草地 >林地。灌木地的导水率是农地的 1.5倍 ,是林地的 3倍多 ;草地和果园的土壤导水率是农地的 1.2倍 ,是林地的 2 .8倍。两种方法测定的入渗率结果具有显著的线性相关性。说明圆盘入渗仪法适合于黄土丘陵区各种利用类型土壤的入渗测定

(AlN)_n团簇的结构与稳定性

用飞行时间质谱法观察到AlN+, Al2N2+等团簇的存在. 结合密度泛函理论的B3LYP/6-31G* 方法, 对(AlN)n (n = 1-15)团簇的几何构型、电子结构和振动频率等性质进行了优化计算, 讨论了化学键的特征和热力学稳定性, 解释了实验质谱. 结果表明：在(AlN)n团簇的基态结构中, 不存在Al—Al和N—N键, Al—N键是惟一键型; (AlN)n结构稳定性的幻数为: 原子数为4, 8, 12, 16, 20, …等4的倍数

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors