NOSES左结直肠癌根治术取标本困难病例的回顾性分析

目的评价经自然腔道取出标本的全腹腔镜下结直肠癌根治术使用蔡氏套管器的临床应用价值。方法通过自制的专利器械——蔡氏套管器,对70例结直肠癌患者,行经自然腔道取出标本的全腹腔镜下结直肠癌根治术,并对患者的临床及手术资料进行回顾性分析。结果 70例患者均在全腹腔镜下完成手术,标本均经过肛门取出。其中降结肠癌7例,乙状结肠癌35例,直乙交界处癌2例,直肠中上段癌26例。无中转开腹和围手术期死亡。通过分析,术前使用\"体质肿瘤指数\"进行患者的评分,评估手术标本取出难易程度,同时,术中使用抵钉座置入等全腹腔镜下手术技术,顺利完成手术。结论经肛门自然腔道取出标本的全腹腔镜下结直肠癌根治术操作安全、创伤小、疼痛轻、更美观,可达到根治性切除的效果。并且可对更高位置的结肠肿瘤如降结肠癌实施全腹腔镜下手术切除,经肛取出标本,在肿瘤根治的基础上进一步提高了微创性。福建省科技计划引导性项目(No.2015D008);;福建省卫生系统中青年人才骨干培养项目(No.2015-ZQN-JC-42

The expression of TNFAIP8 in gastric cancer and its clinical significance

目的通过检测胃癌组织中肿瘤坏死因子α诱导蛋白8(TnfAIP8)的表达,探讨其与胃癌临床病理参数的关系及与胃癌发生发展的关系,揭示胃癌的发病机制。方法采用免疫组化SP法,检测50例胃癌以及50例正常胃粘膜中TnfAIP8的表达。结果 50例正常胃粘膜组织中TnfAIP8无表达,而在50例胃癌组织中TnfAIP8阳性表达13例,TnfAIP8阳性率为26%。低分化胃癌组TnfAIP8阳性表达率明显高于中分化腺癌组(P<0.05);Ⅲ期胃癌组TnfAIP8阳性表达率明显高于Ⅱ期胃癌组(P<0.05);有淋巴结转移组TnfAIP8阳性表达明显高于无淋巴结转移组;TnfAIP8在不同性别和年龄的胃癌组织中无差异。结论 TnfAIP8的表达与患者的性别和年龄无关,而与胃癌的组织学分级、胃癌的TnM分期和淋巴结转移有相关性。TnfAIP8在胃癌组织中表达率较正常胃粘膜升高,差异有统计学意义(P<0.05),提示TnfAIP8可能参与了胃癌的发生、发展。Tumor necrosis factor-α induced protein-8(TNFAIP8) is a recently discovered antiapoptotic molecule.Recent data have demonstrated that TNFAIP8 is involved in the regulation of apoptosis,cellular signaling cascade,tumor proliferation and invasion,as well as metastasis.In this study,we aimed to investigate the expression of TNFAIP8 in patients with gastric cancer and their relationships with clinicopathology.We detected the expression of TNFAIP8 in 50 cases of gastric cancer and 50 cases of normal gastric tissues with SP immunohistochemical.In gastric cancer,the expression of TNFAIP8 was located in cytoplasm and/or nucleolus of tumor cells;the expression of TNFAIP8 in patients with gastric cancer(26%) was higher than that in the normal gastric tissues(0%,P﹤0.05).And the expression of TNFAIP8 in poor differentiated adenocarcinoma of gastric was higher compared with that in well differentiated(P < 0.05),the same difference was fund between Ⅲ andⅡ stages of adenocarcinoma.However,the expression of TNFAIP8 was on significant difference among different person with different age and gender.The results suggest that the expression of TNFAIP8 is not related with patient gender or age,but related with the stage of disease.And the expression of TNFAIP8 may be involved in the generation and development of gastric cancer.福建省自然基金(2010D009

FasL基因构建 蛋白表达及其对胃癌细胞株BGC823和MGC803生长的影响

目的:构建适于原核表达的重组蛋白FasL表达载体,并进行重组蛋白的表达纯化及抗肿瘤活性分析。方法:获得人FasL cDNA全序列,将其分段设计引物,通过重叠PCR获得FasL基因。构建pGEX-5X-1/FasL表达载体。转化大肠杆菌BL21(DE3),IPTG诱导表达,GST柱纯化。采用MTT比色法、流式细胞法检测融合蛋白对胃癌细胞的作用。结果:通过重叠PCR获得了编码正确氨基酸序列的目的基因。表达的目的蛋白表达量占菌体总蛋白的30%以上。纯化后,蛋白纯度达95%以上。MTT比色法与流式细胞技术均表明纯化的融合蛋白能抑制胃癌细胞株BGC823和MGC803的生长,诱导其调亡增加。结论:重组蛋白FasL表达载体的成功构建、表达、纯化及活性分析,为进一步的抗肿瘤功能研究奠定了基础

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies