Design of NMR receive channel based on STM32

针对核磁共振波谱仪中接收通道的功能需要,提出了一种基于STM32作为协处理器,通过移植lWIP协议栈,实现数据流直接多通道网络传输的设计方案。该方案和以往方案相比,大大减轻了主处理器fPgA的工作负担,并且具有实时性高、模块化、易于拓展等特点。主要介绍了该方案的主要设计思路以及软硬件基本架构,重点阐述了其中的关键技术,实物完成后通过nETIO以及系统联机测试的结果表明本方案满足波谱仪接收通道的实际性能需要,同时使得系统的可维护性得到了提高。To satisfy the require of the receiver channel,this paper puts forward a designing program which uses STM32as a co-processor and LwIP as a protocol stack to achieve multichannel data transmission.Compared to the previous programs,this program greatly reduces the workload of the host processor FPGA,It is high real-time,modular and easy to expand.The paper will introduce the software and hardware platform of this program and the key of techniques.The result based on netio and on-line testing proves that this program meets the requirement of receiver channel and improves the manufacturing and maintainability of the system

不同大气CO_2浓度升高处理对水稻秸秆在后茬冬小麦田中分解特性的影响

为研究大气CO_2浓度逐渐增加和稳定高浓度处理对水稻秸秆在后茬冬小麦田土壤中分解特性的影响,进行田间试验,设置3个浓度水平——背景大气CO_2浓度(CK)、每个生长季CO_2浓度比CK逐渐增加40μmol/mol(T1)、每个生长季CO_2浓度均比CK高200μmol/mol(T2),处于上述3个浓度水平下连续3个生长季的水稻秸秆处理编号分别用CK-OTC、T1-OTC、T2-OTC表示,第3个生长季T1-OTC的CO_2浓度为120μmol/mol,3个生长季中前两个生长季处于开顶箱(OTC)外且第3个生长季处于OTC内的处理分别表示为CK、T1、T2.将不同处理下的水稻秸秆埋入麦田土壤中,于填埋后30、60、84、119、149 d测定剩余秸秆的质量以及总碳(TC)、总氮(TN)含量.结果表明:填埋后30 d不同处理下秸秆的分解率为33. 2%～38. 2%,至149 d填埋结束,不同处理下秸秆的分解率为57. 3%～60. 3%.填埋试验后期(填埋后84、119、149 d)的秸秆分解率与粗纤维含量之间存在显著(P0. 05)差异,而OTC-T1处理下水稻秸秆在整个分解阶段的TC含量显著(P<

Purifications and Structure Elucidations of Cyclopenol and Cyclopenin

通信作者：[email protected][中文文摘]肿瘤坏死因子α(TNF-α)是激活的巨噬细胞分泌的一种多功能因子,其过量表达可以引起类风湿性关节炎和其他自身免疫性疾病.因此,寻找开发其小分子抑制剂具有重要意义.在对一株分离自厦门海沧红树林土壤青霉属(Peni-cilliumsp.)真菌的次级代谢产物进行分离纯化以获得TNF-α抑制剂的研究中,通过中压反相硅胶柱层析(RP-18)、常压凝胶柱层析(Sephadex LH-20)、硅胶柱层析(Si-Gel)等柱层析技术对两种产量较高的化合物进行了分离纯化.通过对两个化合物的一维(1D-)、二维(2D-)核磁共振(NMR)和质谱(MS)数据的深入解析,确定两个化合物为生物碱圆弧菌醇和圆弧菌素,两者的产率分别达7.0和3.9mg/L,并通过X射线单晶衍射确定了圆弧菌醇手性碳的绝对构型.圆弧菌醇和圆弧菌素是生物合成TNF-α诱导的人类HIV病毒复制强抑制剂纯绿青霉素过程的中间产物,但是在拮抗过量TNF-α的实验中并没有表现出明显的活性.该研究为开发利用这两种生物碱及通过生物转化合成纯绿青霉素,及加速新的潜在免疫抑制剂的研究提供基础.[英文文摘]Over-expression of the tumor necrosis factor alpha(TNF-α) is responsible for a number of pathological conditions,and TNF-α plays a pivotal role in the origin and progression of rheumatoid arthritis(RA) and other immune mediated disorders,so the development of small-molecule TNF-α inhibitors is significant.In the course of screening the inhibitors of TNF-α from secondary metabolites of microorganisms isolated from marine,two benzodiazepine alkaloids were obtained from the fungus Penicllium sp.,which was isolated from a soil sample collected from Haicang mangrove,Xiamen,China.They were purified with column chromatographies,especially silica gel RP-18,sephadex LH-20 and silica-gel column chromatography.On the basis of thorough analysis of NMR and MS spectra,they were identified as cyclopenol and cyclopenin with high-yield of 7.0 and 3.9 mg/L,respectively.Cyclopenol was further confirmed by a single crystal X-ray diffraction analysis.Cyclopenin and cyclopenol can be converted into the quinolines viridicatin,wich is strong inhibitors of the TNF-α-induced replication of human HIV.Although they showed no significant inhibitory activity of the excessive TNF-α,the determination of absolute configuration is significant to pharmacological study in immune and structural optimization,this maybe help to accelerate the development of potential immune inhibitors.The study of viridicatin maybe help to accelerate the development of potential immune inhibitors.中央高校基本科研业务费专项(2010121092

不同CO_2浓度和N肥水平对粳稻茎鞘非结构性碳水化合物含量与积累的影响

为了解CO_2浓度升高和N肥水平对水稻茎鞘内非结构性碳水化合物(NSC)含量和积累量的影响,利用开顶式气室(OTC),以常规粳稻"南粳9108"为试验材料,设置3个CO_2浓度水平:对照T0(背景大气)、T0+120μmol·mol~(-1)(T1)和T0+200μmol·mol~(-1)(T2)。在OTC内采用盆栽方式,设置3个氮(N)肥水平:10 g N·m~(-2)(N1)、20 g N·m~(-2)(N2)和30g N·m-2(N3)。分别于水稻抽穗期、灌浆期(抽穗后20 d)和成熟期对地上部分各器官生物量、茎鞘NSC含量以及顶部四张叶片的N含量进行分析。结果表明:CO_2浓度升高对抽穗期叶N含量总体无显著影响,但显著降低灌浆期N2和N3水平的叶N含量; CO_2浓度升高对抽穗期茎鞘NSC含量和积累量无显著影响,抽穗期置换到高CO_2浓度环境使灌浆期茎鞘NSC积累显著增加,置换到低CO_2浓度环境使NSC积累显著减少。同一CO_2浓度条件下,NSC含量和积累量均为N1>N2>N3,且N1处理均显著高于N3处理,CO_2浓度升高和N水平的交互作用对灌浆期茎鞘NSC含量影响显著。水稻产量在不同CO_2浓度水平间无显著差异,但随施氮水平的提高而增加。抽穗期与灌浆期水稻茎鞘NSC含量和积累量与茎鞘干重呈极显著正相关,与叶N含量呈极显著负相关;叶N衰减越慢,灌浆期水稻茎鞘NSC残留比(R_(NSC))越低;结实率和产量与R_(NSC)呈显著负相关,R_(NSC)越大,茎鞘NSC转移的越少,结实率和产量越低

Humanization of Therapeutic Antibody of One Hepatitis B Virus and Identification of Its Biological Activity

乙型肝炎病毒(HBV)感染是全球最为重要的公共卫生问题之一,目前临床上治疗HBV的药物在彻底清除病毒方面并没有取得满意的结果,因此迫切需要发展能; 有效清除病毒,尤其是能有效清除乙肝表面抗原(HBsAg)或大幅度降低HBsAg血清学水平的创新性治疗药物.筛选到一株针对HBV和HBsAg上一个; 特定表位的鼠单抗129G1,它能持续有效地清除转基因小鼠体内的HBV及HBsAg,有发展为治疗性抗体药物的潜力.为了降低129G1的免疫原性,采; 用互补决定区(CDR)移植的方式,利用噬菌体抗体库技术对这个鼠单抗进行了人源化,最终得到人源化抗体.人源化抗体与HBsAg的结合活性与嵌合抗体相; 当,抗体质量浓度为1.45; mug/mL时介导THP-1细胞吞噬率达到50%.在HBV转基因小鼠的实验中,获得的129G1人源化抗体能持续有效地抑制血清中HBV; DNA及清除HBsAg.Hepatitis B virus (HBV) infection is a major global health; problem.However,current treatments are not satisfactory in removing; virus thoroughly from patients,thus we need innovative treatment; strategies to remove virus continuously,especially in suppressing the; levels of HBsAg and HBV DNA in vivo profoundly and persistently.In this; study we discovered 129G1 mAb,which is a mouse monoclonal antibody; binding to a special epitope on the hepatitis B surface (HBsAg).129G1; mAb can suppress the levels of HBsAg and HBV DNA in vivo profoundly and; persistently,thus has the potential to be used in drugs of therapeutic; antibodies.In order to reduce the immunogenicity of 129G1 mAb,we; accomplished the humanization of 129G1 using complementarity determining; region (CDR)-grafting and framework region optimization to keep the; original biological function with phage display technology.129G1; humanized antibodies' binding activity with HBsAg is similar to 129G1; cAb.It makes the phagocytosis rate of THP-1 cells up to 50% at; concentration of 1.45 mug/mL.In HBV transgenic mice,the humanized 129G1; exhibited continuous ability in suppressing the HBV DNA in serum and; clearing HBsAg.国家自然科学基金; 福建省自然科学基

CO_2浓度升高和不同氮肥水平下源库处理对粳稻茎鞘非结构性碳水化合物积累和转运的影响

为探究CO_2浓度升高和不同氮肥水平下源库处理对粳稻茎鞘非结构性碳水化合物(NSC)积累和转运的影响,利用开顶式气室(OTC),设置2个CO_2浓度([CO_2]):对照(背景大气,a[CO_2])和在背景大气[CO_2]基础上升高200μmol·mol~(-1)(e[CO_2])。以常规粳稻"南粳9108"为试验材料,在OTC内采用盆栽方式,设置低N(N1,10 g N·m~(-2))、中N(N2,20 g N·m~(-2))和高N(N3,30 g N·m~(-2)) 3个施N水平。抽穗期源库改变设剪叶(LC)和疏花(SR)处理,以不处理为对照。测定并计算了抽穗期和成熟期叶片N含量、茎鞘NSC积累量(TM_(NSC))、NSC表观转运量(ATM_(NSC))及其对籽粒产量的表观贡献率(AC_(NSC))。采用方差分析、相关分析和逐步回归方法对上述观测数据进行分析。结果表明,[CO_2]升高显著降低抽穗期叶片N含量,显著促进中N水平的NSC积累。在不同[CO_2]和N水平下,SR处理均导致成熟期茎鞘TM_(NSC)显著升高,ATM_(NSC)和AC_(NSC)显著降低;在背景大气和不同N水平下,LC处理均显著降低成熟期TM_(NSC),显著提高ATM_(NSC),但[CO_2]升高下LC处理对成熟期TM_(NSC)和ATM_(NSC)均无显著影响。LC处理对籽粒产量及其构成未产生显著影响。粒叶比越高,成熟期TM_(NSC)和千粒重越低,ATM_(NSC)、AC_(NSC)、籽粒产量和收获指数越高。综合影响AC_(NSC)的因素为粒叶比、抽穗期和成熟期TM_(NSC);综合影响籽粒产量的因素为粒叶比、成熟期叶片N含量和TM_(NSC),这些综合影响均可用多元回归模型定量表述

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies