Screening research on effective parts of Alisma orientalis( Samuel. ) Juzep. associated with blood glucose decrease in type 2 diabetic mice

目的初步探索泽泻[Alisma orientalis(Samuel.)Juzep.]降低2型糖尿病小鼠血糖的有效部位。方法采用超声提取法获得泽泻乙醇提取物,再分别用石油醚、乙酸乙酯、水饱和正丁醇和水相进行萃取分离,得到的泽泻乙醇提取物及其各溶剂萃取物应用于腹腔注射烟碱、链脲霉素诱导的2型糖尿病小鼠模型。造模成功后,将动物分组,其中空白对照组(未注射链脲霉素和烟碱的小鼠)和2型糖尿病模型组小鼠连续灌胃0.9%Na Cl溶液21 d(灌胃剂量为10μl/g),测试药物组分别连续灌胃泽泻乙醇提取物、泽泻石油醚萃取物、泽泻乙酸乙酯萃取物、泽泻正丁醇萃取物、泽泻水萃取物21 d(各测试药物灌胃高、中、低剂量分别为100μg/g、50μg/g、25μg/g),阳性对照组连续灌胃罗格列酮溶液21 d(灌胃剂量为10μg/g),每组8只小鼠,筛选具有降低2型糖尿病小鼠血糖的泽泻有效部位。用具有降低2型糖尿病小鼠血糖的有效部位处理3T3-L1前脂肪细胞,观察其对前脂肪细胞分化过程的影响。结果与2型糖尿病模型组比较,连续灌胃泽泻乙酸乙酯萃取物21 d(剂量为100μg/g)降低了2型糖尿病小鼠的血糖,改善了口服葡萄糖耐受试验(oral glucose tolerance test,OGTT)过程中的糖耐量(P<0.01)。1 mg/L和10 mg/L的泽泻乙酸乙酯萃取层均能促进前脂肪细胞的分化过程。结论泽泻乙酸乙酯萃取物降低了2型糖尿病小鼠血糖,促进了3T3-L1前脂肪细胞分化,但其具体作用机制尚需深入研究。Objective To explore the effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice.Methods Ethanol extracts from A. orientalis were obtained by ultrasonic extraction. Then,the ethanol extract was extracted and separated with petroleum ether,ethyl acetate,water saturated n-butanol and water respectively. After decompressing concentration,these solvent extractions were applied to type 2 diabetic mice model induced by intraperitoneal injection of streptozotocin and nicotine. After successful modeling,the mice were divided into the blank control group( without injection of streptozotocin and nicotine),model group of type 2 diabetes mellitus,positive control group and test drug groups,8 in each group. The blank control group and model group were treated with 0. 9% Na Cl at the dose of 10μl / g,the test drug groups were treated with corresponding extracts from different solvent extractions described above at the high-,mid-,low-dose of 100,50,25 μg / g respectively. The positive control group was treated with rosiglitazone at the dose of10μg / g. The treatment course was 21 days for intragastric administration. The effective parts of Alisma orientalis( Samuel.) Juzep. associated with blood glucose decrease in type 2 diabetic mice were screened and then the effect of the screened effective part on the differentiation of 3T3-L1 pre-adipocyte was observed.Results Compared with the model group,the blood glucose was decreased after the intragastric administration with ethyl acetate extract of A. orientalis at dose of100μg / g for 21 days and the glucose tolerance during the oral glucose tolerance test( OGTT) was improved( P < 0. 01). The differentiation of pre-adipocyte was promoted when the ethyl acetate extract of A. orientalis at the concentration of 1 mg / L and 10 mg / L. Conclusion The ethyl acetate extract of A. orientalis reduced the blood glucose in type 2 diabetic mice and promoted the differentiation of 3T3-L1 pre-adipocytes,but the specific mechanism still need to be further studied.福建省科技厅自然科学基金青年创新项目;; 厦门市卫计委资助项目(2014D008),厦门市卫计委资助项目(2015-ZQN-JC-45);; 福建省卫生系统中青年骨干人才培养项目基础项目;; 福建省科技厅自然科学基金(2015J01065);; 福建省教育厅中青年教师教育科研项目杰青项目(JA14421),福建省教育厅中青年教师教育科研项目(JA13425);; 厦门市科技局科技计划高校创新项目(3502Z20143026),厦门市科技局科技计划项目(3502Z20144031);; 厦门医学高等专科学校科研基金(Z2013-12,Z2013-25

构建发展泛珠三角地区产业合作的前提与基础

区域经济合作在市场经济条件下,是商品生产和商品交易发展到一定程度后,不同地区经济主体从而政府基于进一步发展的需要而产生的内生需求。产业协调发展是区域经济合作最重要的内容之一,对泛珠三角经济区的形成具有极为重要的意义。目前而言,不仅整个泛珠三角地区,而且泛珠三角地区内各省区内部的产业分工协作都是相当有限的。在目前条件下,发展泛珠三角地区的产业分工与协作,不是直接从发展产业分工协作入手,而是从其基础:发展经济区内各地区之间的经济贸易联系入手。市场主体在密切经济贸易联系基础上,自然会逐渐产生符合各市场主体利益的产业分工与协作关系

春、秋季台湾海峡海-气CO2通量及其影响因素

于2014年的5月（春季）和9月（秋季）在台湾海峡及其邻近南海和东海海域,采用水气平衡法进行了2个航次的海表和大气pCO2连续走航观测,同时获取了海表温度、海表盐度、风速及气压等数据,并采用海-气CO2分压差减法估算了海-气CO2通量.结果显示,春、秋2个航次平均海表pCO2分别为387±16μatm和408±18μatm.温度是影响台湾海峡及其邻近海域海表pCO2的主控因子,水团混合和其他因素等也对海表pCO2有一定影响.2014年春、秋季节,对研究区域的海-气CO2释放通量的估算结果分别为0.11±1.60 mmol/（m2·d）和2.51±1.10 mmol/（m2·d）.台湾海峡海表pCO2既存在显著的季节变化,又存在较大的空间差异.国家海洋局第三海洋研究所基本科研业务费资助项目(海三科2011015);;厦门南方海洋研究中心资助项目(14GHS016NF16,14PST63NF27

Optimization of β-Cyclodextrin Inclusion Technology of Volatile Oil from Pueraria Compound by Orthogonal Design

目的优选葛根复方挥发油的包合工艺,提高制剂的稳定性。方法采用饱和水溶液法制备包合物,以挥发油中藁本内酯、桂皮醛、甲基丁香酚包合率及包合物得率为综合评价指标,采用正交试验法考察挥发油与β-环糊精的比例、包合时间、包合温度对包合工艺的影响,优化得到葛根复方中挥发油的最优包合工艺。结果葛根复方中挥发油的最佳包合工艺条件为挥发油与β-环糊精比例为1∶8,包合温度为30℃,包合时间为1h,验证试验中,藁本内酯、桂皮醛及甲基丁香酚的包合率为别为86.58%±0.009%,73.34%±0.001%,68.89%±0.020%,包合物得率为67.98%±0.047%。结论所确定的包合工艺挥发油的包合率高、工艺稳定可行。Objective To obtain the optimized technology of volatile oil from Pueraria Compound with β-Cyclodextrin（ β-CD）. Methods The inclusion compound was prepared by saturated peroxide solution method. In order to optimize inclusion technology,orthogonal design was performed,with the content of multi-target ingredient,such as ligustilide,cinnamic aldehyde,methyleugenol and the yield of inclusion as comprehensive evaluation indexes. Three factor was performed,the ratio of volatile oil（ m L） to β-CD（ g）,the inclusion temperature and the inclusion time. Results The optimum conditions for inclusion process were as follow： the ratio of volatile oil（ m L） to β-CD（ g） was 1： 8,the inclusion temperature was 30℃ and the inclusion time was 1h. With the optimum inclusion condition,the content of ligustilide,cinnamic aldehyde and methyleugenol and the yield of inclusion were 86. 58% ± 0. 009%,73. 34% ± 0. 001%,68. 89% ± 0. 020%,and 67. 98% ± 0. 047%,respectively.Conclusion The optimum inclusion condition was stable and reproducible with a high inclusion yield.国家自然科学基金（No.81270901）;上海市教委基金（No.15cxy21

Optimization of extraction for volatile oils and water-soluble constituents in Compound Gegen Decoction

目的优化葛根复方挥发油和水溶性成分提取工艺。方法以浸泡时间、提取时间、加水量为影响因素,综合评分(藁本内酯、桂皮醛、甲基丁香酚含有量,葛根素、阿; 魏酸转移率,干膏得率)为评价指标,正交试验优化提取工艺。结果最佳条件为浸泡时间30 min,提取2次,每次加入10倍量水,第1次提取5; h以收集挥发油,第2次提取1 h以收集水溶性成分,藁本内酯、桂皮醛、甲基丁香酚含有量分别为0.806、1.111、1.635 mg; /g,葛根素、阿魏酸转移率分别为61.62%、73.38%,干膏得率为34.42%。结论该方法稳定可行,可用于提取葛根复方挥发油和水溶性成分。国家自然科学基金; 上海市教委基

Influence of hydrothermal synthesis temperature on the structuresof two 3D coordination polymers

Corresponding author. Tel.: +86 592 2186175; fax: +86 592 2183047.E-mail address: [email protected] (S.-Y. Yang).Two 3D coordination polymers [Zn(mu(4)-dmbdc)](n), 1 and [Zn-5(mu(3)-OH)(4)(mu(5)-dmbdc)(2)(mu(4)-dmbdc)](n), 2 (H(2)dmbdc = 2,5-dimethylbenzenedicarboxylic acid) have been prepared by hydrothermal syntheses with temperature as the only variable. The structure feature is the presence of monoatomic coordination bridges, i.e. mu(3)-OH and mu(2)-O (from mu(3)-COO- of mu(5)-dmbdc) in 2 synthesized at higher temperature. The coordination number of zinc ion and the bridging number of the carboxyl group increase with the increasing synthesis temperature, resulting in the formation of the more stable and condensed phase. (c) 2007 Elsevier B.V. All rights reserved

Remote image classification based on multi-scale boost classifier

遥感图像(RSI)的特殊性使得图像的准确分类变得非常困难。提出了一种自适应多尺度分割的组合分类算法。采用组合分类的办法,也就是将一组功能较弱的分类器联合起来构成一个功能较强的分类器。每一个较弱的分类器都由一级分割来训练并且描述。较弱的分类器可以由线性支持向量机(SVM)和区域距离构成。实验表明该方法能够准确地实现图像的分类并且与实际图像相符。此外,采用分级的多尺度分析方法能够减少训练时间,得到一个性能更好的分类器。仿真表明该方法比其他方法性能更优

Preliminary Analysis on Social Functions of Fujian Broadsword Society from Republic of China to Early Years of New China

民国至新中国成立初期,福建大刀会的组织活动遍及全省各地。着重叙述并分析了福建大刀会活动的社会功能。From the Republic of China to the early years of new China,activities organized by Fujian Broadsword Society were widespread in every corner of Fujian.The paper mainly relates and analyzes the social functions of activities organized by Fujian Broadsword Society

Effects of natural brassinolide on germination,radicle and hypocotyls elongation of five herbage seeds

用9个不同浓度的天然油菜素内酯溶液浸种处理5种牧草种子,观测其对种子发芽和胚根下胚轴伸长的影响。结果表明,0.01 mg/L浓度处理使苜蓿种子发芽率比对照提高了32.2%,发芽天数缩短了1.4 d;0.01mg/L处理使鲁梅克斯种子发芽率增加了27.8%,0.05 mg/L处理使其发芽天数提前了0.8 d;0.03 mg/L处理使普那菊苣种子发芽率提高了28.9%,发芽天数缩短了0.3 d;0.4 mg/L处理使串叶松香草种子发芽率增加了26.7%,发芽天数提前了1.2 d;0.03 mg/L处理使小冠花种子发芽率提高了23.3%,0.05 mg/L使其发芽天数缩短了0.9 d。0.05、0.07、0.03、0.4和0.03 mg/L处理对苜蓿、鲁梅克斯、普那菊苣、串叶松香草和小冠花胚根下胚轴伸长的促进效应最为明显

连通工程对富营养化湖泊沉积物中污染物的影响

为揭示东湖-沙湖连通工程对富营养化湖泊沉积物中污染物的影响,于2013年7月在东湖的水果湖及郭郑湖心采集了20 cm的柱状沉积物,按2 cm分层测定了总有机碳（TOC）、总氮（TN）、总磷（TP）、酸挥发性硫化物（Acid volatile sulfides,AVS）及同步提取金属（Simultaneously extracted metals,SEM）,并与2004、2006和2008年（TOC、TN和TP）及1999年（AVS、SEM）数据进行了比较;同时研究了郭郑湖沉积物氮磷的释放特性。结果表明,（1）水果湖沉积物中污染物含量普遍高于郭郑湖,在连通前均呈增加的趋势;连通后,TOC、TN的平均含量显著降低（P〈0.05）,与2008年比分别降低了25.1%和56.3%（水果湖）及31.9%、66.1%（郭郑湖）;TP平均含量虽略高于2008年,但水果湖沉积物低于2004及2006年,郭郑湖表层沉积物也低于历年,表明TP污染的趋势得到了遏制,湖水流动性的增强有利于沉积物中污染物含量的降低;（2）随着沉积物表层氧化还原状况的改善,表层沉积物AVS含量降低,且最高含量分别由26.4（水果湖）和26.2 mmol/kg（郭郑湖）降低到15.1和18.2 mmol/kg;SEM含量则升高,导致SEM/AVS上升,但SEM/AVS值均小于1,沉积物尚未呈现重金属毒性;（3）东湖沉积物中的氮磷存在潜在的释放风险,其释放与扰动强度有关,且污染物含量高的底层沉积物释放更明显。因此,连通工程有利沉积物中污染物含量的降低,但同时需要采取措施控制污染物释放的风险