基于GA-BP神经网络的塑料X射线吸收光谱的辨识

采集15种塑料样本的X射线吸收光谱(XAS),对光谱数据进行预处理和主成分分析,建立误差反向传播(BP)神经网络和遗传算法优化的误差反向传播(GA-BP)神经网络模型,利用训练集进行网络训练,并通过测试集进行验证。结果表明,GA-BP神经网络相比于BP神经网络可以更好更稳定的对塑料样本的XAS进行识别,这对塑料的回收具有重要的指导意义。国家自然科学基金项目(61571381

Bone Proteomic Analysis About Chinese Medicine Action On Rat Ovariectomy Model of Osteoporosis.

目的:用蛋白质组学的方法对绝经后骨质疏松症和中药作用机理进行研究,有利于临床防治该疾病。方法:建立去卵巢大鼠骨质疏松症模型,设立中药治疗组及假手术对照组,6周后骨质疏松症模型成功,行骨形态学检查,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,分析各组间差异蛋白质点,MADITOF/MS质谱分析,结合蛋白质生物信息库(Matrix science Ltd database),对各蛋白质初步鉴定。结论:鉴定了3个差异蛋白,分别为P1硫氧还蛋白过氧化酶1(Thioredoxin peroxidase 1),P2为阻凝蛋白轻链肽2(Myosin light polypeptide 2),P3为泛素化酶E2-17KD(ubiquitin-conjugating enzyme E2-17kD)。初步认为这三种蛋白质在绝经后骨质疏松症的发病及中药治疗过程中发挥着重要调控作用。Objective:To study the mechanism about osteoporosis of OVX(ovariectomy) and Chinese Medicine action on osteoporosis in proteomics,it is of benefit to prevent and cure this disease.Methods:Bilateal OVX in rats was performed as osteoporosis model.Rat was randomly divided into three groups:control group,osteoporosis model group and Chinese Medicine therapy group.The pathology of bone was examined after 6 weeks.In the present proteomic study,we characterized the protential effects of OVX and Chinese Medicine on protein expression in rat bones.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusions:Three proteins were identified as proteins similar to thioredoxin peroxidase 1,Myosin light polypeptide 2 and ubiquitin-conjugating enzyme E2-17kD.These proteins have been demonstrated to be postmenopausal proteins.These results can provide valuable experimental evidences for the research for the molecular mechanism of osteoporosis which was response to OVX and Chinese Medicine in bone.国家自然科学基金项目(基金号:30400606); 广东省自然科学基金项目(基金号:04010036);; 广东省科技厅项目(基金号:粤科社字2004,139号);; 广州中医药大学创新基金项目(基金号:K004044

Bone Proteomic Analysis About Chinese Medicine Action on Rat Glucocorticoid-induced Model of Osteonecrsis.

目的:用蛋白质组学的研究方法进行激素性骨坏死的病理和中药作用机理的研究,有利于临床治激素性骨坏死。方法:按照常用激素性骨坏死的造模方法,建立大鼠坏死模型,并设立中药治疗组及空白对照组,经过6周处理后经骨形态学检查,确定骨坏死造模成功,处死动物取股骨和肱骨,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,找到各组间差异蛋白质点,进一步行胶内酶切,M AD ITOF/M S质谱分析,得到各差异点的蛋白质肽指纹图谱,结合蛋白质生物信息库(M atrix sc ience L td database),对各蛋白质进行初步鉴定。结论:初步鉴定了3个差异蛋白,分别为阻凝蛋白重链ⅡB、磷脂谷胱甘肽过氧分酶及泛素化酶E 2(MW:17 kd),初步认为这三种蛋白质在激素性骨坏死的发病及中药治疗过程中发挥着重要调控作用。Objective:To study the mechanism about glucocorticoid-induced osteonecrosis and Chinese Medicine action on osteonecrosis in proteomics,it is beneficial to prevention and cure this disease.Methods:Rat was randomly divided into three groups:cortrol group,osteonecrosis model group and Chinese Medicine therapy group.In the present proteomic study ,we characterized the potential effects of glucocorticoids and Chinese Medicine on protein expression in rat bone.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusion:Three proteins were identified as proteins similar to Myosin heavy chain ⅡB,Phospholipid hydroperoxide glutathione peroxidase and ubiquitin-conjugating enzyme E2-17kD.These proteins have been documented to be glucocorticoid-relted proteins.These results can provide valuable experimental evidences for the research of the molecular mechanism of osteonecrosis response to glucocorticoids and Chinese Medicine in bone.获得国家自然科学基金资助(30273631,3010244);; 广东省自然科学基金(020778);; 广东省教育厅基金(Z02011

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

The Methods of Abstracting Chinese Dot Matrix for Embedded Systems

嵌入式系统人机交互性能的重要指标之一是汉字显示打印功能,利用汉字点阵实现此功能是一种简单实用的方法,因此从现有汉字系统中提取汉字点阵是嵌入式系统开发的第一步工作。提取汉字点阵有两种方法,一是从点阵字库中提取,二是从矢量字库中提取,这两种方法在实际开发工作中都得到了应用。详细分析了这两种方法的原理及编程实现,提出了自动扫描嵌入式应用程序生成汉字点阵的方法,这种方法特别适用于建立小型汉字点阵库。It′s a simple and practical method to use the Chinese dot matrix to display and print Chinese characters in embedded systems.Therefore,abstracting dot matrixes from the existing Chinese character OS is the first step of developing embedded system.We can abstract dot matrixes from the dot matrix library or from the vector library.Authors have applied both ways in practical applications.This paper analyses the principles and programming of both ways in detail.In addition,this paper proposes a method of auto-scanning source files,this method is best for creating mini dot matrix library.厦门大学985二期信息创新平台项目资

The Method of Extracting the Protein Specimen from Rat Bone for Further Proteomics Analysis

蛋白质组学是目前研究的重要热点.该文拟建立大鼠骨组织蛋白质样品提取的最佳方法,用于双向电泳分离,为进一步进行蛋白组学分析奠定基础.实验对3种不同方法制备的样本进行同一条件的二维电泳(2- DE)图谱,比较2- DE的结果.结果发现,丙酮沉淀法最佳.这些结果提示,只有结合骨组织成分特征与双向电泳的具体要求,选择恰当的样品提取液,采用合理的提取步骤,获得蛋白质样品,才能得到清晰高分辨率的电泳图谱,满足蛋白质组学分析需要.Proteomics is a challenging field that has been growing rapidly in the postgenomic era.Protein preparation is a key procedure for two-dimensional electrophoresis (2-DE) proteomics.In the current study,three procedures of protein sample preparation,namely basic,dialysis and acetone methods,were compared for establishment of an optimal method to extract proteins from rat bone,based on 2-DE maps from protein samples prepared by the three methods.Of the three methods,acetone method is the best one.Using this method to purify proteins from rat bone,high quality of peptide mass fingerprint can be achieved.The procedure of the method is as follows.Prepared bone (1.0 g) was placed in an earthen bowl to grind into powder in liquid nitrogen and then 3 mL ice-cold buffer (1.44 g urea,0.46 g sulfocarbamide,0.12 g CHAPS,0.047 g DTT,0.015 g Tris-base,30 μL 100 mmol/L PMSF) was added to it.After stored at 4℃ overnight,these samples were centrifuged at 12 000 g for 30 min.The supernatant was dialyzed against 0.01 mol/L PBS for 24 h.Then ten times of volumes of cooled acetone were added to the specimen for the concentration of proteins.Followed by the store at -20℃ for 2 h,the proteins were obtained by centrifugation and then dry at 4℃.Before the isoelecric focusing,the sample was redissolved in the buffer (7 mol/L urea,4% CHAPS,100 mmol/L DTT,40 mmol/L Tris,2 mol/L sulfocarbamide,0.5% ampholyte (pH 5~7),1 mmol/L PMSF) at the room temperature for 2 h.The results may provide a valuable method of sample preparation for study of bone proteomics.国家自然.3010244);; 广东省自然科学基金(020778);; 广东省教育厅基金(Z02011)资

川西亚高山5种森林生态系统的碳格局

采用样方法研究了川西亚高山白桦(Betula platyphylla)林(BF)、针阔混交林(MF)、岷江冷杉(Abies faxo-niana)林(FF)、紫果云杉(Picea purpurea)林(SF)和方枝柏(Sabina saltuaria)林(CF)的碳贮量、组成及其分布格局。结果表明:1)在5种森林生态系统中,土壤碳含量和碳贮量都随土壤深度的增加而极显著地降低,且与土壤深度之间有较好的线性关系;2)地被物碳贮量分别为SF(23.97±1.77)>FF(21.35±3.64)>MF(11.7

四川王朗自然保护区地被物水源涵养能力评价

采用实地调查和水浸法,研究了王朗保护区7种典型生态系统(草地(GL)、灌丛(SH)、白桦林(BF)、混交林(MF)、冷杉林(FF)、云杉林(SF)和方枝柏林(CF)的地被物层贮量吸水过程与最大储水量,并以此为基础评价该区域地被物层的水源涵养能力。结果表明:(1)不同生态系统地被物层贮量及其最大储水能力差异较大,其顺序依次为SF>FF>MF>CF>BF>SH>GL和SF>FF>MF>CF>SH>BF>GL;(2)在地被物的吸水过程中,储水量(Q)和吸水速率(S)与浸泡时间(t)的关系可分别用Q=c ln(

The Application of Proteomics on Modernized Study of TCM

蛋白质组学是后基因组时代的最重要研究课题,能够深化对生命现象本质的了解。对于中医药现代化研究,蛋白质组学作为高通量平行筛选技术在理论体系上与中医药的整体观相契合,能够深化证候的内在本质研究及中药复方的组成与作用机理研究,并能有力促进中药新药开发随着蛋白质组学相关技术的发展完善,其对中医药现代化研究的帮助将进一步扩大。Proleomics is the most important subject of postge-nomic period, which can deepen the understanding of the essence of life. In the modernized study of TCM, the high - flux paralled -screening technique of proteomics in coincident with the wholistic concept of TCM, and can deepen the study of intrinsic quality of TCM syndrome and the composition and mechanism ol herbal formula and strongly promote the development of new herbal medicine. Following with the development and consummation of proteomics and its relevant technique, the modernized study will be beneficial and further developed.国家自然科学基金(编号:30273631) 广东省自然科学基金(编号:020778