Analysis and Design of the Logistics Management System for a College

在高校的信息化管理中，后勤管理是一项重要的内容。在所有的后勤工作中，事务性工作的重要性越来越突出，同时它也变得越来越复杂。要想提高后勤管理的效率，必须从事务性工作入手。另外一方面，随着网络和数据库技术的快速发展，推动了信息化管理系统的发展，在很大程度上提高了管理的效率。因此，将网络和数据库技术应用到高校后勤管理系统中，必然可以提高高校后勤管理的效率。 本文基于此背景，分析并设计了一套高校后勤管理系统，本文的工作内容主要分为三个方面：首先，从理论上详细的分析了网络和数据库等相关技术的内涵，包括系统基本技术、系统开发工具、SQLServer2008技术等等；其次，进行了高校后勤管理系统的需求分析...In the process of information management of the colleges, the role of the logistics management is very important. Of all the logistics work, the importance of the transactional work is more and more prominent, and it also becomes more and more complex. To improve the efficiency of the logistics management, it must be started from transactional work. On the other hand, with the rapid development of...学位：工程硕士院系专业：软件学院软件工程系_工程硕士(软件工程)学号：X201223026

Analysis and Design of the Logistics Management System for a College

在高校的信息化管理中，后勤管理是一项重要的内容。在所有的后勤工作中，事务性工作的重要性越来越突出，同时它也变得越来越复杂。要想提高后勤管理的效率，必须从事务性工作入手。另外一方面，随着网络和数据库技术的快速发展，推动了信息化管理系统的发展，在很大程度上提高了管理的效率。因此，将网络和数据库技术应用到高校后勤管理系统中，必然可以提高高校后勤管理的效率。 本文基于此背景，分析并设计了一套高校后勤管理系统，本文的工作内容主要分为三个方面：首先，从理论上详细的分析了网络和数据库等相关技术的内涵，包括系统基本技术、系统开发工具、SQLServer2008技术等等；其次，进行了高校后勤管理系统的需求分析...In the process of information management of the colleges, the role of the logistics management is very important. Of all the logistics work, the importance of the transactional work is more and more prominent, and it also becomes more and more complex. To improve the efficiency of the logistics management, it must be started from transactional work. On the other hand, with the rapid development of...学位：工程硕士院系专业：软件学院软件工程系_工程硕士(软件工程)学号：X201223026

Optimization of Gene Engineering Bacteria Fermentation Conditions for Expression of Nucleoside Phosphorylase

目的优化核苷磷酸化酶(包括嘌呤和嘧啶核苷磷酸化酶)基因工程菌的发酵表达条件。方法通过工程菌摇瓶培养,测定吸光度d值,考马斯亮蓝(brAdfOrd)法测定蛋白,SdS-PAgE电泳和凝胶成像扫描分析表达量,优化表达条件;通过正交试验优化50 l发酵罐发酵条件。结果摇瓶培养起始PH为7.0~7.2,于30℃培养4 H,加入终浓度为0.4 MMOl/l的异丙基硫代半乳糖苷(IPTg)诱导8 H后收获菌体,可得到较高的生物量和重组酶蛋白表达量。50 l发酵罐的最佳条件为起始PH为7.0~7.2,于32℃培养4 H,加入终浓度为0.4 MMOl/l的IPTg诱导9 H后收获菌体,每升发酵液可得2 g以上的酶蛋白。结论基因工程菌发酵表达核苷磷酸化酶产量较高,可工业化生产,为酶法合成核苷酸类似物的研究奠定了基础。Objective To optimize the fermentation conditions of gene engineering bacteria for expression of nucleoside phosphorylase( including PNPase and PyNPase).Methods By adopting the engineering bacteria shaking culture,the absorbance D value was determined,the protein was measured by the Bradford method,the expression quantity was detected by the SDS-PAGE electrophoresis and the gel-imaging scanning,so the expression conditions were optimized; the orthogonal experiment was adopted to optimize the fermentation condition of the 50 L fermentor.Results The initial pH was 7.0-7.2 in the shaking culture and the incubation was performed for 4 h under 30 ℃,then isopropyl thiogalactoside( IPTG) with a concentration of 0.4 mmol / L was added for 8 h induction and the bacteria were finally harvested,which could obtain higher biomass and recombinant enzyme protein expression quantity.The experimental results revealed that the optimal conditions of the 50 L fermentor were as follows: with the initial pH value of 7.0-7.2,the incubation lasted for 4 h under 32 ℃, the bacteria were harvested after adding 0.4 mmol / L IPTG for 9 h induction.Each liter of fermentation broth could obtain at least 2 g apoenzyme.Conclusion The expression of gene engineering bacteria can obtain the higher output of nucleoside phosphorylase,which can be used for the industrial production and establishes the basis of synthesizing the nucleotide analogues by the enzymatic method

维甲酸诱导 HL-60 细胞凋亡的研究

目的: 观察维甲酸(RA)在体外诱导 H L-60 细胞(人早幼粒细胞白血病细胞株)凋亡的作用, 探讨维甲酸治疗早 幼粒细胞白血病的作用机制。方法: 用 DNA 电泳、DNA 片段定量测定技术、流式细胞术分析及光镜和电镜观察凋亡细胞。结 果: 在体外培养中加维甲酸 50 mg/L 孵育 4 h, DNA 片段率达 55. 7%±12. 1%, 而对照为 12. 5%±4. 9%(P <0. 001, n = 9); 流式细胞术检测发现, 在 50 mg/L 维甲酸作用下, 细胞凋亡达 50%, 对照为 9%。电镜观察维甲酸组 H L-60 细胞出现典型 的凋亡细胞形态改变。维甲酸诱导 HL-60细胞凋亡具有剂量及时间依赖性,维甲酸作用 6 h,出现凋亡高峰; 随维甲酸浓度升 高,诱导凋亡作用明显增强。在环孢素 A( CsA)存在时,低浓度的维甲酸也有明显诱导 HL-60 细胞凋亡作用。结论: 维甲酸在 体外能诱导 HL-60 细胞凋亡, CsA 能增强维甲酸诱导 HL-60 细胞凋亡活性。研究提示诱导白血病细胞凋亡是维甲酸治疗急 性早幼粒细胞白血病的重要机制之一

头颈部动脉支架植入围手术期患者血清NETs标志物的动态变化（Dynamic Changes in Serum NETs Markers during the Perioperative Period in Patients Undergoing Head and Neck Arterial Stent Implantation）

目的 探讨头颈部动脉支架植入后血清中性粒细胞外陷阱（neutrophil extracellular traps，NETs）标志物瓜氨酸化组蛋白3（citrullinated histone 3，cit-H3）及髓过氧化物酶（myeloperoxidase，MPO）水平随时间变化的规律。 方法 前瞻性连续纳入因头颈部动脉粥样硬化性大动脉狭窄行支架植入术的患者，分别于术前空腹24 h内，术后6、12、24、48 h采集患者静脉血，测定血清cit-H3及MPO水平。采用经Greenhouse-Geisser校正的单因素重复测量方差分析，比较患者围手术期不同时间点血清cit-H3及MPO水平的动态变化趋势。根据支架植入部位（颅内 vs. 颅外）、支架植入数目（1个 vs. 2个）、责任血管是否为症状性狭窄，以及手术时脑梗死是否处于急性期（病程≤14 d），对血清cit-H3及MPO水平的变化进行亚组分析。 结果 本研究共纳入48例患者，平均年龄为（61.8±8.4）岁，男性42例（87.5%）。在整体患者中，血清cit-H3在术前及术后6、12、24、48 h的水平分别为（54.50±6.48）ng/mL、（56.73±6.50）ng/mL、（71.27±7.35）ng/mL、（53.53±17.35）ng/mL和（52.22±5.45）ng/mL；血清MPO在术前及术后6、12、24、48 h的水平分别为（25.45±6.67）ng/mL、（26.29±6.75）ng/mL、（28.28±7.68）ng/mL、（31.55±9.09）ng/mL和（28.68±7.61）ng/mL，两者术后水平均呈现先升高后降低趋势，整体差异均具有统计学意义（P=0.002；P=0.022）。颅外及颅内支架亚组、1个及2个支架亚组、症状性狭窄亚组及非急性期亚组的血清cit-H3水平均于术后12 h达到峰值。颅内支架亚组的血清MPO水平于术后12 h达到峰值，2个支架亚组、症状性狭窄亚组及非急性期亚组的血清MPO水平均于术后24 h达到峰值。 结论 头颈部动脉支架植入术后48 h内，血清NETs标志物cit-H3和MPO水平呈现先升高后恢复至术前水平的变化趋势，这种趋势在症状性狭窄患者中更显著。（Abstract: Objective To explore the dynamic changes over time in serum levels of neutrophil extracellular traps (NETs) markers—citrullinated histone 3 (cit-H3) and myeloperoxidase (MPO)—after head and neck arterial stent implantation. Methods Patients with atherosclerotic stenosis of the head and neck arteries who underwent stent implantation were prospectively and consecutively enrolled. Venous blood samples were collected from the patients within 24 hours of fasting before surgery, and at 6, 12, 24, and 48 hours after surgery to measure serum cit-H3 and MPO levels. One-way repeated measures analysis of variance with Greenhouse-Geisser correction was used to compare the dynamic changes in serum cit-H3 and MPO levels across different perioperative time points. Subgroup analyses of the changes in serum cit-H3 and MPO levels were conducted based on stent implantation site (intracranial vs. extracranial), number of stents implanted (1 vs. 2), whether the responsible vessel had symptomatic stenosis, and whether cerebral infarction was in the acute phase (disease duration≤14 days) at the time of surgery. Results A total of 48 patients were enrolled in this study with a mean age of (61.8±8.4) years, including 42 males (87.5%). Among all patients, the serum cit-H3 levels before surgery and at 6, 12, 24, and 48 hours after surgery were (54.50±6.48) ng/mL, (56.73±6.50) ng/mL, (71.27±7.35) ng/mL, (53.53±17.35) ng/mL, and (52.22±5.45) ng/mL, respectively. The serum MPO levels at the corresponding time points were (25.45±6.67) ng/mL, (26.29±6.75) ng/mL, (28.28±7.68) ng/mL, (31.55±9.09) ng/mL, and (28.68±7.61) ng/mL, respectively. Both markers showed a trend of increasing first and then decreasing, with statistically significant overall differences (P=0.002 for cit-H3; P=0.022 for MPO). In the extracranial and intracranial stent subgroups, the single and double stent subgroups, the symptomatic stenosis subgroup, and the non-acute phase subgroup, the serum cit-H3 levels peaked at 12 hours after surgery. The serum MPO levels peaked at 12 hours after surgery in the intracranial stent subgroup. However, in the double stent subgroup, the symptomatic stenosis subgroup, and the non-acute phase subgroup, the serum MPO levels peaked at 24 hours after surgery. Conclusions Within 48 hours after head and neck artery stent implantation, the serum levels of NETs markers cit-H3 and MPO showed an initial increase followed by a gradual return to preoperative levels. This trend was more pronounced in patients with symptomatic arterial stenosis.

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel