Cellulase Production by Solid-state Fermentation and Saccharification Using the Fibre of the Energy Plant Erianthus arundinaceum

作者简介:张树河(1975 年—),男， 助理研究员。研究方向： 能源植物的选育及转化制生物燃料。 通讯作者： 龙敏南， E-mail:[email protected][中文文摘]以能源植物斑茅(Erianthus arundinaceus)为材料,对其纤维成分、产酶条件和糖化条件进行了分析。结果表明:斑茅含有纤维素43.77%、半纤维素28.57%、木质素11.01%;对斑茅进行预处理后,纤维中纤维素的含量提高至72.54%,半纤维素含量降低至13.02%,木质素的含量降低至3.34%。利用预处理的斑茅纤维进行固态发酵产酶,其最佳的产酶条件为:茅麸比1∶3,固液比1∶2,氮源(脲)0.75%,初始pH 5.0,32℃发酵培养72 h;相对于利用未经预处理的斑茅纤维为原料,其FPA酶活提高210.9%,达到20.74 U/g,CMC酶活提高239.1%,达到218.26 U/g。以纤维素酶对斑茅纤维进行糖化,糖化率达到40.60%。[英文文摘]Using energy plant Erianthus arundinaceus as the material,we analysed the fiber composition,enzyme production conditions and saccharification.The results showed that Erianthus arundinaceus consisted of 43.77% cellulose,28.57% hemicellulose and 11.01% lignin.After pretreatment,the cellulose content increased to 72.54%,while the hemicellulose and lignin content decreased to 13.02% and 3.34%,respectively.The optimal condition for solid-state fermentation to produce cellulase was: ratio of pretreated cellulose fibre to wheat bran 1 ∶ 3, ratio of solid to liquid 1:2, nitrogen source(urea)0.75%, initial pH 5.0, temperature 32 ℃ and fermentation time 72 h. When using the pretreated fibre as the carbon source, the FPA activity and CMC activity increased 210.9% and 239.1%, respectively. The pretreated fibre was used for saccharification under the optimal condition with a yield of 40.60%.国际科技合作重点项目(No.2009DFA60930);; 国家自然科学基金(No.31170067)资

日本の混雑空港における発着枠(タイム・スロット)配分の在り方 : アカデミックな視点からのあるべき姿

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回避的不適応行動(症状)の臨床的研究 -機能分析と介入法を考える-

This study revealed that most maladjustment behaviors had avoidance mechanism. These behaviors included anxiety disorders, somatoform disorders, dissociative disorders, alcohol dependence, social withdrawal, school refusal and the like. In the functional analysis of this article, we argued that the forms of psychopathology were viewed as unhealthy methods of avoidance. The treatments of avoidance-based maladjustment behaviors (symptoms) expose clients to previously avoided private experiences