作者简介:张树河(1975 年—),男， 助理研究员。研究方向： 能源植物的选育及转化制生物燃料。 通讯作者： 龙敏南， E-mail:[email protected][中文文摘]以能源植物斑茅(Erianthus arundinaceus)为材料,对其纤维成分、产酶条件和糖化条件进行了分析。结果表明:斑茅含有纤维素43.77%、半纤维素28.57%、木质素11.01%;对斑茅进行预处理后,纤维中纤维素的含量提高至72.54%,半纤维素含量降低至13.02%,木质素的含量降低至3.34%。利用预处理的斑茅纤维进行固态发酵产酶,其最佳的产酶条件为:茅麸比1∶3,固液比1∶2,氮源(脲)0.75%,初始pH 5.0,32℃发酵培养72 h;相对于利用未经预处理的斑茅纤维为原料,其FPA酶活提高210.9%,达到20.74 U/g,CMC酶活提高239.1%,达到218.26 U/g。以纤维素酶对斑茅纤维进行糖化,糖化率达到40.60%。[英文文摘]Using energy plant Erianthus arundinaceus as the material,we analysed the fiber composition,enzyme production conditions and saccharification.The results showed that Erianthus arundinaceus consisted of 43.77% cellulose,28.57% hemicellulose and 11.01% lignin.After pretreatment,the cellulose content increased to 72.54%,while the hemicellulose and lignin content decreased to 13.02% and 3.34%,respectively.The optimal condition for solid-state fermentation to produce cellulase was: ratio of pretreated cellulose fibre to wheat bran 1 ∶ 3, ratio of solid to liquid 1:2, nitrogen source(urea)0.75%, initial pH 5.0, temperature 32 ℃ and fermentation time 72 h.
When using the pretreated fibre as the carbon source, the FPA activity and CMC activity increased 210.9% and 239.1%, respectively. The pretreated fibre was used for saccharification under the optimal condition with a yield of 40.60%.国际科技合作重点项目(No.2009DFA60930);; 国家自然科学基金(No.31170067)资
