Study on HMW-PAHs metabolic pathway and transcriptional regulation in Novosphingobium pentaromativorans US6-1

多环芳烃（PAHs）是一类在环境中广泛分布的有机化学污染物，具有“三致”（致癌、致畸、致突变）效应，通过食物链的传递会对生态环境和人体健康造成极大危害。目前研究结果表明，微生物的降解与转化是消除PAHs污染的有效方式之一。以苯并芘（BaP）为代表的高分子量PAHs（HMW-PAHs）因其结构稳定、难降解，可在环境中长期存在。能以BaP为唯一碳源和能源进行矿化的微生物研究报道较少，代谢路径也不够清楚。本论文以一株筛选自海洋环境的、高效的PAHs降解菌NovosphingobiumpentaromativoransUS6-1为模式菌株，结合实时荧光定量PCR技术和链特异转录组测序技术，针对其对菲（...Polycyclic aromatic hydrocarbons (PAHs) are one class of organic pollutants which are widely distributed in the environment. They are carcinogenic, teratogenic, and mutagenic to living organisms through food chain. It is an efficient way to recover PAHs contamination by microbial remediation. Currently, there is only limited information regarding to the bacterial biodegradation pathway and physiol...学位：理学硕士院系专业：生命科学学院_水生生物学学号：2162011115246

The unique role of sleep problems among symptoms of posttraumatic stress disorder: A cross-lagged panel network analysis

借助交叉滞后网络的分析方法,探讨睡眠问题在创伤后应激障碍(PTSD)的症状系统中与其他症状的格兰杰因果关系。以经历舟曲泥石流的1460名儿童青少年为研究对象,在灾后3, 15和27个月对其PTSD症状进行测量。交叉滞后网络分析结果显示：3到15个月时的睡眠问题的发出预期影响最高;而15到27个月时与他人疏离和线索引发生理反应的发出预期影响最高。结果表明了睡眠问题对PTSD症状影响的时间特异性,并为儿童青少年的PTSD干预方案和诊断模式提供了启示。</p

Chemotaxis and characteristics of chemotactic genes in Novosphingobium strains

【目的】选择了几株隶属于新鞘氨醇杆菌属(Novosphingobium)的有和没有多环芳烃(PAHs)降解能力的细菌,在基因组水平比较分析它们的; 趋化通路,并探究其中一些菌株对芳香化合物和三羧酸(TCA)循环中间代谢产物的趋化性。【方法】通过基因组的比较分析,研究了几株新鞘氨醇杆菌的趋化蛋; 白组成和趋化基因分布,并采用滴定法和游动平板法检测了相关菌对芳香族化合物和TCA循环中间代谢产物的趋化性。【结果】N.; pentaromativorans US6-1、N. pentaromativorans F2、Novosphingobium indicum; K13、 Novosphingobium stygium DSM 12445、Novosphingobium sp.; C2AC等5株新鞘氨醇杆菌对芳香族化合物和TCA循环中间代谢物具有不同程度的趋化性;所选的7株已完成基因组测序的新鞘氨醇杆菌N.; pentaromativorans F2、N. pentaromativorans US6-1、Novosphingobium sp.; PP1Y、Novosphingobium sp. AP12、Novosphingobium sp. Rr; 2-17,Novosphingobium sp. B-7和Novosphingobium sp. DSM; 19370均含有趋化蛋白MCP、CheW、CheA、CheB、CheR和CheY,且亲缘关系最近的US6-1、F2和PP1Y; 3株菌的che基因簇中的基因排列一致,为cheW-Y-D-B-R-A-(X);新鞘氨醇杆菌的趋化系统属Fla类型。; 【结论】几株新鞘氨醇杆菌都具有较为完整的趋化通路,且对多种芳烃及其代谢物的趋化性各不相同,其中菌株US6-1趋化现象最明显。[Objective] The present study aims to analyze the chemotaxis genes and; proteins of several PAH-degrading Novosphingobium strains, and the; chemotaxis of these strains toward aromatic compounds and intermediates.; [Methods] Based on genome comparative analysis, we identified the; chemotaxis genes organization and proteins distribution. We used drop; and swarm plate assays to detect the chemotaxis of these strains toward; aromatic compounds and intermediates of TCA cycle. [Results] We found; that all these Novosphingobium strains showed chemotaxis, but the; chemotatic ability varied. The completed genome sequenced strains N.; pentaromativorans F2, N. pentaromativorans US6-1, N. pentaromativorans; PP1Y, Novosphingobium sp. AP12, Novosphingobium sp. Rr 2-17, and; Novosphingobium nitrogenifigens DSM 19370 contained MCP, CheW, CheA,; CheB, CheR and CheY. Strain F2, US6-1 and PP1Y, shared a consistent; order of chemotaxis genes in che cluster. The chemotatic system of these; Novosphingobium strains belonged to the Fla chemotactic system.; [Conclusion] These strains all contained a complete chmotaxis pathway.; Their chemotactic ability toward aromatic compounds and intermediates; varied, and the chemotaxis of US6-1 was obvious.国家自然科学基金; 福建省自然科学基

Prediction of Energy Resolution in the JUNO Experiment

International audienceThis paper presents the energy resolution study in the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The study reveals an energy resolution of 2.95% at 1 MeV. Furthermore, the study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data taking. Moreover, it provides a guideline in comprehending the energy resolution characteristics of liquid scintillator-based detectors