The Study on the Secondary Metabolites from Seven Strains of Marine Fungi

近年来，由于从陆生生物资源发现新的活性代谢产物的几率越来越低，人们开始把研究热点转移到了生态和物种远比陆地生境复杂多样的海洋。海洋真菌作为海洋生境的重要成员，由于其在海洋生境中所处的独特地位，具有特殊的代谢途径，能够产生结构独特、骨架新颖、活性多样的次级代谢产物，已成为天然产物研究中一个引人注目的焦点。 本文对来源于福建海域的7株海洋真菌(4株红树植物内生真菌HTF3,BYY-1,A-1-5-4,C-2-4-7和3株海藻共附生真菌hzla01-1,PT2,B5)的次级代谢产物进行了研究，从其发酵产物中分离鉴定了61个化合物(包括5个乙酰化产物)，其中有15个是新化合物，有7个化合物为首次从红...In recent years, the hot spot has been focused on the much more complex marine resources because of the decrease of discovering novel bioactive metabolites from terrestrial organisms. During adapting to oceanic ecosystem, marine fungi can produce numerous secondary metabolites with unique structures, novel skeletons and varied bioactivities. So marine fungi have attracted more and more attention o...学位：理学博士院系专业：生命科学学院生物化学与生物技术系_微生物学学号：B20042601

A Novel Sesquiterpene Glucoside from Trichoderma sp. PT2

从海藻真菌木霉TrICHOdErMA SP.PT2菌体提取物中分离到一个新倍半萜糖苷类化合物TrICHOdErMOSIdE(1)。并通过1d-,2d-nMr波谱数据鉴定了化合物的结构,并应用MTT法对化合物1的细胞毒活性进行了研究。A novel sesquiterpene glucoside,trichodermoside(1),was isolated from the mycelia extract of algicolous fungus Trichoderma sp.PT2.The structure was elucidated by spectroscopic analyses,including 1D-,2D-NMR data and mass-spectrometric analyses.Compound 1 displayed weak growth inhibition against human HeLa cells.supportedbyChinaOceanResourceR&DAssociation(GrantDYXM-115-02-2-13);theNationalHighTechnologyResearchandDevelopmentProgramofChina(863;No.2006AA09Z410

Study on the Isolation Identification and Antitumor Activity of a Phenol Derivative from Mangrove Fungus BYY-1

[作者简介] 杜希萍( 1978—) ，女，讲师，从事天然产物化学方面的研究． 通讯作者: 苏文金( 1956—) ，男，教授，从事海洋药物与食品方向研究，E-mail: wjsu@ jmu. edu. cn．[中文文摘]采用硅胶柱层析、薄层层析、Sephadex LH-20柱层析从红树内生真菌BYY-1的次级代谢产物中分离到化合物B1.通过乙酰化、NMR、HR ESI-Q-TOF MS等技术鉴定了化合物B1的结构,确定其为首次从红树内生真菌中分离到的化合物.运用MTT法和流式细胞术研究了化合物B1的抗肿瘤活性,结果表明,化合物B1可显著抑制Hela细胞的增殖[英文文摘]Compound B1 was obtained from the secondary metabolites of mangrove fungus BYY －1 using silica gel column chromatography，thin layer chromatography and Sephadex LH － 20 column chromatography．The structure of compound B1 was elucidated by acetylation，NMR and HR ESI －Q －TOF MS analysis，and compound B1 was isolated from mangrove fungi for the first time． The antitumor activity of compound B1 was studied by the methods of MTT and flow cytometric DNA analysis． The results showed compound B1 could significantly inhibit the proliferation of Hela cells with IC50 3. 3 μg /mL，and induce evident Hela cells apoptosis when compound B1 was 4 μg /mL．中央高校基本业务费资助项目(2010121092); 福建省科技计划重点项目(2011N1008);; 集美大学博士启动基金资助项目(ZQ2011004

The Effects of Different Seawater Concentration and Culture Time on Antimicrobial Activity of Marine Fungi

作者简介: 杜希萍女,讲师。现从事天然产物化学方面的研究。E-mail: xipingdu@ jmu.edu.cn 通讯作者。E-mail: wjsu@ jmu.edu.cn[中文文摘]用滤纸片琼脂扩散法研究不同海水浓度和培养时间对海洋真菌抗菌活性的影响,旨在为大规模培养海洋真菌和提高获得抗菌物质的几率提供理论依据。结果显示:用不同海水浓度培养基发酵海洋真菌,在供试的10株海洋真菌中,5株海洋真菌的抗菌活性和抗菌谱有明显差异;培养时间不同,6株海洋真菌的抗菌活性差别较大。实验结果表明,不同海水浓度和培养时间对海洋真菌抗菌活性有显著影响。[英文文摘]The effects of different seawater concentration and culture time on antimicrobial activity of marine fungi were studied using agar diffusion method with filter paper diskettes,aiming at providing theoretical foundation for culturing marine fungi on a large scale and improving the probability of antimicrobial metabolites.The results showed that the differences of antimicrobial activities and antimicrobial spectrum of five among ten tested marine fungal strains cultured in different seawater concentration media were fairly obvious; antimicrobial activities of six marine fungal strains were significantly different at the different culture time.The study indicated different seawater concentration and culture time greatly affected the antimicrobial activity of marine fungi．集美大学博士启动基金(ZQ2011004);中央高校基本业务费(2010121092)资

Effects of different kinds of media on the growth and active metabolite production of algicolous marine fungus PT2

本文采用PD、GPY、YES、SYE、GPM 5种不同培养基培养海藻真菌菌株PT2,并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析,旨在了解不同培养基对菌株生长和活性物质合成的影响.结果显示,PT2菌株在不同培养基上的培养特征及生长速率有所不同,液体培养基发酵液抽提物的抗肿瘤活性也有较大差别,其中SYE发酵液抽提物活性最强,对KB、Raji和Hela　3种肿瘤细胞的IC50分别为 7. 6μg/cm3、2. 4μg/cm3和 3. 3μg/cm3.采用HPLC法对不同抽提物进行分析,发现它们之间的组成成分有显著差异.本文的实验结果表明,不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响.Algicolous marine fungus PT2 was cultivated on five different kinds of media which are PD, GPY, YES, SYE and GPM. The growth status, secondary metabolite production of fungus PT2 and anti-tumor activities of extracts from fungus PT2 were analyzed to study the effects of different media on growth and active metabolite production of fungus PT2. The results showed that it was different in culture characters and growth rates of PT2 on different media. There were also various inhibiting effects on tumor cells by five culture broth extracts, the activity of extract from SYE was highest in KB,Raji and Hela tumor cells, IC_(50) was 7.6μg/mm~3, (2.4μg/mm~3) and (3.3μg/mm~3) respectively. Culture extracts of fungus PT2 from five media were determinated by HPLC method and there was significant difference in compositions of extracts. The results indicated that there are distinct effects of different media on growth and active metabolite production of PT2.国家高科技研究发展计划资助项目(2001AA620401);; 福建省自然科学基金资助项目(C0210001

The Effects of Different Media on Antitumor Active Substances Production of Mangrove Fungus HTF3

对红树林真菌HTF3菌株在PD、GPY、YES、GPM及SYE 5种不同培养基中的生长情况、抗肿瘤活性及代谢产物的形成进行了研究,旨在了解培养基对该菌株生长及抗肿瘤活性物质合成的影响.结果显示:在不同的培养基中,HTF3菌株的生长速率不同,抗肿瘤活性及抗瘤谱也有明显差异;采用HPLC对不同培养基发酵液乙酸乙酯抽提物进行分析,发现不同抽提物的色谱峰数、色谱峰的保留时间及紫外吸收值均有较大的不同.实验结果表明:不同培养基对HTF3菌株抗肿瘤活性物质的合成有显著影响.Mangroves are special woody plant communities in the intertidal zone of tropical and subtropical coasts.Mangrove fungi growing in mangroves are attractive because a variety of chemically interesting and biologically significally substances have been purified from their secondary metabolites.Therefore,this paper studied the growth,antitumor activities and metabolites production of mangrove fungus HTF3 on potato dextrose medium,glucose peptone yeast extract medium,yeast extract sucrose medium,glucose peptone malt extract medium and starch yeast extract medium,to find out the effects of different media on the growth and antitumor active substances production of HTF3.The results showed that the growth rates of HTF3 on different solid media were different;the growth rates of HTF3 on glucose peptone yeast extract medium and starch yeast extract medium was 17.1mm/d and 11.1 mm/d;antitumor activities and antitumor specffum were markedly different in different liquid media,the IC_(50) against Raji cell of five extracts from different fermentation broth,namely A(PD),B(GPY),C(YES),D(GPM) and E(SYE) is 31.9,66.5,76,49.6 and 27.6 μg/mL respectively.The ethyl acetate extracts of the fermentation broth from different media were analysed using HPLC.The numbers of chromatographic peak,retention times and UV spectra were significant different.The study indicated diffetent media greatly affected the antitumor active substances production of HTF3.国家863计划(2001AA620401);; 福建省科技计划项目(2004N003)资