Advances in Pathobiology of Alveolar Echinococcosis

泡状棘球蚴病 (alveolarechinococcosis,AE)是一种重要的人兽共患寄生虫病。本文综述了其病原泡状棘球蚴的地理分布、宿主类别、传播情况及其发育生物学方面的研究情况 ,指出研究病原生物学的现实意义和今后仍需努力的方向。The alveolar echinococcosis (AE),one of the most dangerous parasitic zoonosis in many parts of the world,is caused by infection with larva of Echinococcus multilocularis. This review mainly summarizes the recent pathobiology knowledge of AE: the etiologic agents;the geographic distribution throughout the north hemisphere;the intermediate host and definite host;the life-cycle pattern;and the developmental biology of the parasite. Meanwhile,in order to prevent and control this disease,the current significance and its research prospect are discussed

p38MAPK induces apoptosis of glioma cell

目的　研究 p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响 .方法　利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系 C6中 ,用免疫细胞化学染色检测其在细胞转染前后的表达情况 ,用 HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响 .结果　转染 p CMV5 - p38MAPK质粒组 p38MAPK蛋白表达阳性 ,细胞形态发生变化 ,贴壁性降低 ,出现大量凋亡细胞 .结论　转染 p38MAPK基因可诱导胶质瘤细胞凋

Regulation of Cell Cycle of Glioma C6 Cells by Regulator of G Protein Signaling 16

目的　探讨G蛋白调节子 16(RGS16)对胶质瘤C6细胞周期的影响。方法　利用脂质体介导法将RGS16基因导入C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁生长情况 ;免疫细胞化学法检测转染前后RGS16蛋白的表达情况 ;流式细胞仪检测转染pCMV 5 RGS16和 pCMV 5质粒后每隔 12h后的细胞周期变化。 结果　转染pCMV 5 RGS16质粒 2 4h后 3 0 .0 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ,72h之后细胞又恢复正常 ;RGS16蛋白的表达呈时相性 ,3 6h时表达率最高 (阳性率为13 .0 % ) ,72h表达终止 ;C6细胞的各期细胞比例变化与RGS16蛋白表达对应 ,在 3 6h时G1期比例从转染前的 70 .5 %降低到60 .2 % ,S期比例从 2 0 .9%增加到 3 4.9% ;在 48h时G1期增加到 76.2 % ,S期减少到 11.4% ;72h各期恢复到正常比例。对照组细胞转染前后形态变化不明显 ,RGS16蛋白表达阴性 ,细胞周期变化不明显。结论　RGS16能促进C6细胞周期的运行 【英文摘要】 Objective To study the effect of regulator of G protein signaling 16(RGS16) on the cell cycle of glioma C6 cell.Methods pCMV5 RGS16 was transfected into C6 cells by lipofectin.The morphological and adhesive changes of the cells were observed under an inverted microscope.Expression of RGS16 was examined by immunocytochemical method both before and after the transfection.Flow Cytometry was adopted to measure the fraction number changes of the cell cycle phase every 12 h.Results 24 hours after the transfec..

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家

Promotion of glioma C6 cells proliferation by overexpressed RGS16

目的　探讨 G蛋白调节子 16 (RGS16 )对大鼠胶质瘤C6细胞的生物学特性的影响 .方法　利用脂质体介导法将RGS16基因导入 C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁情况 ;3H- Td R法检测 C6细胞在转染不同梯度p CMV5 - RGS16和 p CMV 5质粒后的增殖情况 ;免疫细胞化学法检测转染前后 RGS16蛋白的表达情况 ;流式细胞仪检测转染 p CMV5 - RGS16和 p CMV5质粒 36 h后细胞周期变化和细胞是否有凋亡发生 .结果　转染 p CMV5 - RGS16质粒 2 4 h后 30 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ;RGS16蛋白表达阳性 ;3H- Td R法检测显示 C6细胞增殖速度与转染p CMV5 - RGS16的量呈正相关 ;细胞周期结果显示 G1期细胞百分数减少 10 % ,而 S期细胞百分数增多 14 % ;未发现RGS16与凋亡有直接关系 .结论　 RGS16可能促进 C6细胞的增殖 . 【英文摘要】 s: AIM To study the effect of RGS16 on the biological characteristics of glioma C6 cells. METHODS pCMV5 RGS16 was transfected into C6 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an inverted microscope. Proliferation of C6 cells was measured by 3H thymidine ( 3H TdR) assay after gradient transfections of pCMV5 RGS16 and pCMV5. Expression of RGS16 was examined by immunocytochemical method both before and after the transfection. Flow cytometry ...高等学校骨干教师计划资

キムチが有効なカルシウム源となるための最適保存条件(人間環境学)

キムチ中に多く含まれるCaに着目し,市販キムチの購入後の保存に伴う品質変化を調べ,最適保存条件について検討を行った。また,有機酸濃度とCaの可溶化率との関係を調べ,キムチ中のCaの可溶化メカニズムを考察した。4℃保存ではpH,酸度が最適な状態で長く保たれ,この点から見ると4℃保存が品質保持に最も適していると考えられた。塩辛中に含まれると考えられる炭酸カルシウムやリン酸カルシウムの可溶化率は,有機酸濃度が上昇するにしたがって高くなったが,実際のキムチ中のCaの水溶性比率は有機酸濃度が上昇しても高くはならなかった。この理由としては,カルシウムイオンとキムチ中の何らかの物質の結合が起こり不溶化が進んだことが考えられる。いずれの保存温度においてもキムチ中のCaの60～70%は可溶化された状態であり,Ca源として有効であると考えられる。市販キムチの最適保存条件の決定のためには,Ca不溶化のメカニズムの解明はもちろんのこと,官能検査など更なる検討が必要である。There are many fermented foods in East Asian countries. Kimchi which is Korean traditional fermented vegetable food, has many functional properties such as anticancer, antibacterial, antimutagenic, etc. There are few studies on either storage condition after purchased or changes of calcium (Ca) form during storage. The purposes of this study are to investigate the quality changes during storage period and to examine the optimum storage conditions that Kimchi can be available source for Ca supply. Kimchi was purchased Korean Kimchi on the market. It was stored at 4℃, 20℃, 30℃ up to 7 weeks. The changes of pH, acidity, organic acid content, ratio of soluble and insoluble Ca form in Kimchi were measured. 1. The pH decreased and acidity of Kimchi increased during storage at 4℃. Their optimum pH was 4.2～4.5 and acidity was 0.4～0.8 %. 2. Lactic acid content increased during storage at each temperature. Especially, it was found that lactic acid content was 2.3g/100g f.wt. for 1 week storage at 30℃. 3. Ca content of Kimchi was 28mg/100g f.wt. and 70% of this was water soluble form. There were few changes at 4℃ and 20℃. The ratio of soluble Ca increased in first storage period and decreased in the middle period. On the latter, it\u27s ratio increased again at 30℃ storage. The optimum storage of Kimchi is a good condition at 4℃ on the point of view of pH, acidity and ratio of soluble Ca

日本オリンピック委員会における情報戦略活動

This study summarizes the outline of the ststematic information strategy activities done by Japan Olympic Committee(JOC), which aims to achieve success in the international competitions such as the Olympics. This study shows the things mentioned below: 1) JOC systematically tackles the improvement of international competitiveness, using the word "information strategy" officially. 2) JOC s core organization to tackle the improvement of international competitiveness is "information strategy section" which works as a substructure of the Information and Medical Science Special Committee. 3) "Information strategy section" is a section entrusted as a think-tank for JOC\u27s improvement of international competitiveness. 4) The main roles of "information strategy section" should be to (1) analyze the actual situation (2) make and propose a plan (3) check and evaluate (4) provide information (5) support NF