Effect of oleoylethanolamide on expression of vascular cell adhesion molecule-1 in human umbilical vein endothelial cells

目的探讨油酰乙醇胺(OEA)对肿瘤坏死因子α(TNF-α)诱导的人脐静脉内皮细胞血管细胞黏附分子-1(VCAM-1)表达的影响。方法体外培养人脐静脉内皮细胞,分别加入3种不同浓度的OEA(10,50,100μmol/L)或非诺贝特(10,50,100μmol/L)共同孵育10 h,再加入TNF-α共同孵育6 h,采用实时定量逆转录聚合酶链式反应和酶联免疫吸附剂检测测定VCAM-1以及mRNA和蛋白的表达,并采用Western blot方法检测过氧化物酶体增殖物激活受体α(PPAR-α)的蛋白表达。结果与非诺贝特相比,不同浓度的OEA更加显著地抑制人脐静脉内皮细胞VCAM-1mRNA和蛋白的表达,随着浓度的增大,抑制作用逐渐增强。Western bolt结果显示OAE能明显增强PPAR-α蛋白的表达。结论OEA对TNF-α引起的内皮细胞受损起到保护作用,其机理可能与上调过PPAR-α有关。 【英文摘要】 Purpose To investigate the effect of oleoylethanolamide(OEA) on tumor necrosis factor α(TNF-α)induced the expression of vascular cell adhesion molecule-1(VCAM-1) in human umbilical vein endothelial cells(HUVECs).Methods OEA and fenofibrate of different concentrations were incorporated in HUVECs for 10 hours respectively.Human recombinant TNF-α was then incubated with HUVECs for 6 hours.The expression of VCAM-1 in mRNA level and protein level was detected by real-time quantitative RT-PCR and enzyme linked im...厦门大学“活性有机小分子的合成化学与化学生物学”创新团队项目;; 厦门市科技局科技计划350Z20083007项

专业化视域下图书馆员的专业素养及其形成

针对新的江西省专业技术资格任职条件中关于图书资料专业研究馆员(副)资格条件出现的新变化,从专业道德、专业知识和技能、科学研究3个方面,阐述了图书馆员专业素养的形成策略

信息公平视域下知识转移的影响因素

信息公平是人类社会普遍追求的价值目标所在,知识转移是实现信息公平的有效途径。在诸多影响知识有效转移的因素中,最为重要的当属信息权利、分配制度及社会关系,也即笔者所述的认知情境

东昆仑-阿尔金山地区黑颈鹤种群分布与秋季数量变化/Distribution and Population Size of Black-necked Cranes in Autumn in East Kunlun and Altun Mountains, Xinjiang[J]

黑颈鹤(Grus nigricollis)是青藏高原特有物种,在新疆主要分布在与青海、西藏相邻的阿尔金山、东昆仑山地区.2011年9 ～11月,对该地区黑颈鹤的分布、种群大小、数量变化、生存状况等进行了详细调查.在乌尊硝尔、铁木里克乡、玉素甫阿勒克、鸭子泉、阿达滩、祁曼塔格乡、吐拉牧场等25个样点,都观察到有黑颈鹤分布.利用样点调查法和直接计数法,重复调查164次,共记录到黑颈鹤158只.其中,在依协克帕提湿地(N37°15′～37°23 ′,E90°11′ ～90°20′,海拔3903 m)最多一次记录到126只黑颈鹤集群.结合早期的科学考察记录,推测在整个东昆仑-阿尔金山地区共有黑颈鹤220～260只左右.黑颈鹤家庭成员数量为1～4只,4种类型的家庭所占比例分别为5.9％、60.3％、29.4％和4.4％.在10月份之前,主要以家庭为单位活动；10月中旬,开始大规模集群,10月29日集群数量达到最高峰,并开始迁徙；11月6日黑颈鹤全部迁徙离开.此外,还观察到有少量的灰鹤(G.grus)和蓑羽鹤(Anthropoides virgo)与黑颈鹤混居在一起

阿尔金山-昆仑山鸟类区系调查/Survey of Avifauna in Kunlun and Altun Mountains[J]

2010～2012年连续3年调查新疆阿尔金山国家级自然保护区及周边包括昆仑山、祁曼塔格山、库木库里盆地的鸟类资源.共录得鸟类16目38科90属166种,约占新疆鸟类总数的36.6％.其中,金腰燕(Hirundo daurica)、冕柳莺(Phylloscopus coronatus)、日本松雀鹰(Accipiter gularis)为新疆首次记录.考察区介于青藏区与蒙新区之间,鸟类区系特点表现为高地型(23种,占13.9％)与中亚型(约49种,占29.9％)相互渗透,而北方型(69种,占42.1％)虽然排在首位,但多数是旅鸟,东洋型(1种,占0.6％)的种类比较罕见.依协克帕提湿地是鸟类的一个繁殖地,也是迁徙的重要驿站

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies