DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

Biodiversity of the ectomycorrhiza on the rare and endangered tree species Tuga chinensis tchekiangensis(Flous) Cheng in Wuyishan Nature Reserve

对武夷山国家级自然保护区残遗与濒危树种南方铁杉植物群落历经了6年的调查,共采集了123种外生菌根,经显微与超显微技术鉴定了84种,这一结果是迄今为止已发表的,在同一树种中菌根类型最多的记录.它不仅证明了武夷山地区的菌物区系由于处于东西相连,南北贯通的位置上,而且从区系成分上据有温热两带俱全的特点,因而形成生物多样性,并从共生菌的角度再度揭示了物种协同进化的规律.Systematic investigation was made on ectomycorrhizae in the woods of a variety of Tsuga chinensis,tchekiangensis(Flous) Cheng,in Wuyishan Nature Reserve in the past six years.During that period,120 ectomycorrhiza samples were collected and 84 types identified by analysing their macro-micro-and ultrastructure.This is the first time that so many types of mycorrhiza in the forests of the species are reported on.This result has proved Wuyishan's geographical position at the cross where a fungi growing area links the east and west,south and north.Thus the region owns the characteristics of both the temperate and the subtropical zone and it is thus such peculiarity whereby its biodiversity is created.Moreover,considering the results of this investigation in the regard of symbiosis between plants and fungi,their co-evolution is ascertained once more.国家自然科学基金资助项目(39770004);; 福建省自然科学基金资助项目(C97003,D0410002

一种逐次逼近模数转换器

本发明揭示了一种逐次逼近模数转换器,其内集成有电容阵列,其特征在于:该电容阵列内的电容分为三级:数字信号输出端的最高位至第五位设为第一级、第四位与第三位设为第二级,第二位与最低位设为第三级——选定第一级电容中第七位的电容为单位电容,高于第七位的第m位电容为由2m-7个单位电容并联而成,第六位和第五位的电容分别为由2个和4个单位电容串联而成,第二、三级的电容连接方式与第五、六位的相同,各级内每一位的电容相互并联,相邻两级电容阵列间均串联有3个单位电容。通过对电容阵列以分级连接的结构形式加以改进,能有效减少电容数目,减小电容面积,提高匹配精度,从而实现该模数转换器低功耗、高匹配度、低寄生及低面积间的折中

使用双程序计数器计算待处理指令地址的系统

本发明公开了一种使用双程序计数器计算待处理指令地址的系统,包括第一程序计数器模块、指令译码单元、执行单元、存储单元、写回单元和链接寄存器;其中,所述写回单元包括第二程序计数器模块,所述第一程序计数器模块为系统取指令功能模块,所述第二程序计数器模块为写回级程序计数器模块,实现下一条待完成指令的地址计算。本发明通过写回单元中的第二程序计数器模块,可以在中断控制或异常处理中中为中断控制器或者异常处理提供精确的无需处理的返回地址,确保精确返回地址的正确唯一性,简化了一般设计中的中断程序与异常处理程序的返回程序,缩短中断异常处理的时间