台湾海峡悬浮颗粒中的甲藻指示物──甾醇(4α,23,24-trimethyl-5α-cholest-22-en-3β-ol)

检出台湾海峡两个季节的表层水悬浮颗粒样品中的甾醇4α，23，24-TrIMETHyl-5α-CHOlEST-22-En-3α-Ol，该组分被认为可用来指示甲藻.分析结果表明：表层海水悬浮颗粒中该组分含量出现两次最大值，其规律与甲藻的昼夜垂直移动规律相一致；该组分在台湾海峡夏季含量比冬季要高，但仍明显低于同期的厦门附近海域的含量，这种季节变化及海域差别与所报道的甲藻的情况基本一致.国家教委、福建省科委重点科研和国家教委海洋生态环境开放研究实验室资

Soil heterotrophic respiration in Casuarina equisetifolia plantation at different stand ages

以中国亚热带木麻黄沿海防护林为研究对象,在2006年5月-2007年4月,利用lI-8100土壤呼吸自动观测系统对不同林龄(幼林、中林、成林)木麻黄人工林生态系统的土壤异养呼吸特征进行了监测。结果表明,不同林龄木麻黄林地土壤异养呼吸季节动态均呈单峰曲线,最大值出现在6~7月份,最小值则出现在12~1月份。土壤温度和水分对土壤异养呼吸的季节变化存在显著影响,并有明显的交互作用,进行单因素方差分析发现,土壤异养呼吸季节变化与5CM深的土温存在着较好的指数相关关系(P成林>幼林。随着林龄的增大,土壤异养呼吸对土壤温度的敏感性增强。土壤异养呼吸对土壤总呼吸的贡献在幼林、中林、成林中分别达到71.89%、71.02%和73.53%。幼林、中林、成林样地土壤异养呼吸CO2年释放量分别为29.072、38.964和30.530T·HM-2·A-1。The soil respiration rates (Rh) in 6-year-old (young), 17-year-old (middle-age), 31-year-old (mature) Casuarina equisetifolia coastal plantations were measured using an LICOR-8100 automated soil CO2 flux system from May 2006 to April 2007.Results show that Rh displayed an obvious seasonal pattern across the observed years.The maximum values of Rh occurred at June and July and the minimum at December and January.Soil temperature and soil moisture as well as their interaction had significant effects on the monthly dynamics of Rh.The analysis by one-way ANOVA showed that Rh had a significantly exponential relation (pmature plantation>young-age plantation.With the increase of growth age of plantation, the Q10 of Rh increased.The contribution of Rh to total soil surface CO2 flux was 71.89%, 71.02% and 73.53% for the young, middle-age and mature plantation, respectively.It was estimated that the annual CO2 fluxes from Rh were 29.07, 38.964 and 30.530 t·ha-1·a-1 for the young, middle-age and mature plantation, respectively.supportedbyKeyProjectsintheNationalScience&TechnologyPillarProgramduringtheEleventhFive-yearPlanPeriod(Nos.2006BAD03A14-01);ImportantScience&TechnologySpecificProjectsofFujianprovince(2006NZ0001-2);supportedbytheKeyLaboratoryofsouthmountaintimberculti-va

Study on organochlorine pesticide and PCBs at surface water in Xiamen Harbour

利用GC -ECD对厦门西港 1998年 7月取得的 9个站位表层水体中的 18种有机氯农药 (HCHs ,DDTs等 )和 12种多氯联苯 (PCBs)进行分析。其中有机氯农药的浓度范围 6 .6 0～ 32 .6ng/L(其中HCHs:3.5 1～ 2 7.8ng/L ,均值 8.5 7ng/L ;DDTs:0 .95～ 2 .2 5ng/L ,均值 1.45ng/L) ,多氯联苯的浓度为 0 .0 8～ 1.6 9ng/L ,同国内外其他港口海区相比较 ,其污染程度相对较低。同时 ,对有机氯污染的分析表明 ,发现近年来仍有有机氯的污染输入 ,其农药的使用主要集中在六六六和滴滴涕上 ;多氯联苯和有机氯农药在厦门西港有着相似的分布及来源特征。organochlorine pesticides and 12 polychlorinated biphenyls(PCBs) at the sruface water in Xiamen Harbour were determined. The range of organochlorine pesticides was 6.60～32.60 ng/L, and the PCBs concentrations were 0.08～1.69 ng/L. Compared with the results of other harbor and estuaries, it showed that the contamination for organic chlorine pollutants was more slight than those of others. At the same time, it was found that organochlorine pesticides and PCBs have the similar geological destribution and resources.福建省自然科学基金!项目 (D9810 0 0 3

The trends and characteristics of organochlorines pollution in surface sediments of Xiamen Western Bay

利用GC ECD对厦门西港 1 998年 7月取得的 8个站位表层沉积物中的 1 8种有机氯农药 (HCHs,DDTs)等和 1 2种多氯联苯 (PCBs)进行分析 .其中有机氯农药的浓度范围ND(未检测出 )— 0 58ng/g(其中HCHs和DDTs的含量分别为ND—0 1 4ng/g与ND— 0 0 6ng/g) ,多氯联苯的浓度为ND— 0 32ng/g ,与 1 986、1 993年的厦门西港的分析结果相比较 ,污染程度明显降低 ;说明近年来的厦门的有机污染得到逐步控制 ,也与有机物随时间推移逐渐详解有关 ,分析表明多氯联苯和有机氯农药在厦门西港有着相似的分布特征 .The contents of 18 organochlorine pesticedes and 12 polychlorinated biphenyls(PCBs) in suface sediments from Xiamen Western Bay were analyzed by GC\|ECD. The range of organochlorine pesticides was ND—0 58?ng/g,and the PCBs were ND—0 32?ng/g.Compared with the results of Xiamen Harbor in 1986 and 1993, it showed that the pollution of organochlorines decreased gradually along the time, in Xiamen Harbor became and the contamination was more slightly than that of other Harbors or Estuaries. At the same time, it was found that the geological distributions of organochlorine pesticides and PCBs, also the HCHs and DDTs were similar to each other, which indicated that the polltion was cansed by a same source

Evaluation and Fate of the Organic Chlorine Pesticides at the Waters in Jiulong River Estuary

1999 0 6,对九龙江口 1 5个站位的表层水 ,1 3个站位的间隙水进行了 1 8种有机氯农药的测定结果表明 ,有机氯农药总含量在表层水中的浓度范围为 51 3～ 2 4 79ng L ;在间隙水中的浓度范围是 2 66～ 33355ng L .对不同有机氯的含量在各站位的分布特征进行了探讨 ,发现Methoxychlor(甲氧滴涕 ) ,EndosulfanSulfate(硫酸硫丹 ) ,Endrinaldehyde(乙醛异狄氏剂 )以及EndosulfanII(硫丹 ) ,Dieldrin(狄氏剂 ) ,Deta HCH和Beta HCH 7种有机氯农药在 1 8种有机氯农药中都占主要部分 ;九龙江口的六六六的含量顺序 :β >δ α >γ ;对于滴滴涕 ,表层水中的含量 :DDE DDD >DDT ;间隙水中的含量 :DDE DDT >DDD ,二者DDE的含量都在总DDTs的 50 %以上 ,说明环境中的DDTs主要降解为DDE ;九龙江口有机氯农药随着盐度梯度 ,在河口中呈去除趋势 ;且间隙水中有机氯农药比表层水中的浓度高 ,说明其倾向于吸附在沉积物颗粒上 ,其浓度差使得有机污染物可能通过再悬浮等过程从底层向上层迁移 .九龙江口的有机氯农药污染与其他港湾相比 ,污染水平相当 ,部分站位水质有机氯农药 (HCHs和DDTs)超过国家一类水质的标准 .Organochlorine Pesticides at the water(surface water and porewater) in Jiulong River Estuary were determined. The range of organochlorine pesticides at surface water was 51.3～2479ng/L, and the concentration in porewater was 266～33355ng/L. The results showed that the organochlorine pesticides were removed in the estuary and the sediments were the reservior of the organochlorine pesticides. Compared with the results of other harbor and estuaries , it showed that the contamination for organic chlorine pollutants in Jiulong River Estuary was similar to those of others. At the same time,the risk of organochlorine pesticides in the estuary was evaluated.1998年教育部重点项目、基金委资助留学人员短期回国工作讲学专项基金!项目联合资助 (4 9910 76 0 74

基于证券实验室的会计专业相关课程教学改革研究

由厦门大学会计发展研究中心提供的第四届会计与财务问题国际研讨会——会计教育改革与发展论文集中的第二部分：教学研究10随着科学技术的进步，目前会计专业的教育观念、人才培养模式、教学内容、教学方法和手段凸显滞后。改革教学方法和手段，加强学生获取知识的能力和创新精神的培养，是深化高等学校会计专业教学改革的重要内容，对提高教学效率、保证教学质量具有重要意义。本课题正是基于这样的背景，在证券实验室的环境下从课程设置、教学方式和师资教育等方面探讨新的会计类课程的教学模式。 会计类课程的教学方法改革，主要体现在演示教学、交互探讨式教学和模拟现实教学；会计的教学手段创新，建立会计模拟作账实验室、会计电算化实验室、会计及相关课程的模拟交易实验室和开发会计教学软件等。本课题研究针对我国高等教育会计专业教学中存在的会计专业课程设置过细、教学方式单一、会计模拟实践教学硬件落后和师资缺乏等问题，基于证券模拟实验室的功能开发和项目建设，提出课程设置的调整方案，建立以“公共基础课＋专业理论课（专业基础课、专业课）＋专业实践课＋公共选修课”的课程设置基本模式，重新整合课程内容体系，以模拟试验室为基础建立课程“套餐”，以多媒体教学推进教学方式的改革。证券模拟实验室为科研提供所需的大批数据，科研所需的检验论证直接通过模拟实验室模拟运行，通过这种“教学+科研”的形式组织学生进行科研实验，在提升教师素质的同时，增强学生的专业素质和科研能力

Promotion of glioma C6 cells proliferation by overexpressed RGS16

目的　探讨 G蛋白调节子 16 (RGS16 )对大鼠胶质瘤C6细胞的生物学特性的影响 .方法　利用脂质体介导法将RGS16基因导入 C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁情况 ;3H- Td R法检测 C6细胞在转染不同梯度p CMV5 - RGS16和 p CMV 5质粒后的增殖情况 ;免疫细胞化学法检测转染前后 RGS16蛋白的表达情况 ;流式细胞仪检测转染 p CMV5 - RGS16和 p CMV5质粒 36 h后细胞周期变化和细胞是否有凋亡发生 .结果　转染 p CMV5 - RGS16质粒 2 4 h后 30 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ;RGS16蛋白表达阳性 ;3H- Td R法检测显示 C6细胞增殖速度与转染p CMV5 - RGS16的量呈正相关 ;细胞周期结果显示 G1期细胞百分数减少 10 % ,而 S期细胞百分数增多 14 % ;未发现RGS16与凋亡有直接关系 .结论　 RGS16可能促进 C6细胞的增殖 . 【英文摘要】 s: AIM To study the effect of RGS16 on the biological characteristics of glioma C6 cells. METHODS pCMV5 RGS16 was transfected into C6 cells by lipofectin. The morphological and adhesive changes of the cells were observed under an inverted microscope. Proliferation of C6 cells was measured by 3H thymidine ( 3H TdR) assay after gradient transfections of pCMV5 RGS16 and pCMV5. Expression of RGS16 was examined by immunocytochemical method both before and after the transfection. Flow cytometry ...高等学校骨干教师计划资

Regulation of Cell Cycle of Glioma C6 Cells by Regulator of G Protein Signaling 16

目的　探讨G蛋白调节子 16(RGS16)对胶质瘤C6细胞周期的影响。方法　利用脂质体介导法将RGS16基因导入C6细胞中 ,在倒置显微镜下观察细胞形态变化和贴壁生长情况 ;免疫细胞化学法检测转染前后RGS16蛋白的表达情况 ;流式细胞仪检测转染pCMV 5 RGS16和 pCMV 5质粒后每隔 12h后的细胞周期变化。 结果　转染pCMV 5 RGS16质粒 2 4h后 3 0 .0 %细胞贴壁性降低 ,突起收缩 ,细胞变圆 ,72h之后细胞又恢复正常 ;RGS16蛋白的表达呈时相性 ,3 6h时表达率最高 (阳性率为13 .0 % ) ,72h表达终止 ;C6细胞的各期细胞比例变化与RGS16蛋白表达对应 ,在 3 6h时G1期比例从转染前的 70 .5 %降低到60 .2 % ,S期比例从 2 0 .9%增加到 3 4.9% ;在 48h时G1期增加到 76.2 % ,S期减少到 11.4% ;72h各期恢复到正常比例。对照组细胞转染前后形态变化不明显 ,RGS16蛋白表达阴性 ,细胞周期变化不明显。结论　RGS16能促进C6细胞周期的运行 【英文摘要】 Objective To study the effect of regulator of G protein signaling 16(RGS16) on the cell cycle of glioma C6 cell.Methods pCMV5 RGS16 was transfected into C6 cells by lipofectin.The morphological and adhesive changes of the cells were observed under an inverted microscope.Expression of RGS16 was examined by immunocytochemical method both before and after the transfection.Flow Cytometry was adopted to measure the fraction number changes of the cell cycle phase every 12 h.Results 24 hours after the transfec..

Effects of nitrogen and potassium on photosynthetic characteristics in summer maize leaves under long-term water stress

采用2种不同夏玉米基因型(陕单9号,抗旱品种;陕单911,不抗旱品种)的盆栽试验,研究了长期水分胁迫下氮、钾对各生育期叶片净光合速率、蒸腾速率、胞间二氧化碳浓度和叶绿素含量的影响,旨在从光合生理特性揭示这些因子的抗旱机理。结果表明,长期水分胁迫下叶片净光合速率,蒸腾速率、胞间二氧化碳浓度(除成熟期)和叶绿素含量显著降低,不抗旱品种降幅更甚。抗旱品种的净光合速率和叶绿素含量大于不抗旱品种,而蒸腾速率和胞间二氧化碳浓度则相反。两品种苗期光合作用较弱,净光合速率和叶绿素含量均较低,抽雄期达到高峰。施氮能不同程度降低水分胁迫下玉米叶片的蒸腾速率,增加叶绿素含量.提高净光合速率,从而减缓水分胁迫对光合作用的伤害。随氮肥用量增加,不抗旱品种净光合速率和叶绿素含量显著升高,蒸腾速率和胞间二氧化碳浓度明显降低,两种氮肥用量间有显著差异;抗旱品种在低氮用量时效果显著,但高低氮用量间无显著区别。钾对受水分胁迫的玉米表现出比氮肥更突出的效果。相反,在适量供水条件下,氮、钾肥的作用明显下降。以上结果表明,适当用量的氮、钾肥可以有效地改善水分胁迫下作物叶片的光合特性,从而增强作物的抗旱性