Design and Implementation of A Company Production Management System

随着计算机和网络技术的进步，各大制造型企业已广泛且深入地开展了信息化建设工作，通过将信息技术应用到采购、生产、加工、销售等环节，从而达到提高企业生产效率，增强企业自身竞争力的目的。杭州科德磁业有限公司是一家从事磁性材料的研发、生产和销售为一体的磁业公司，成立于2005年，经过十年的发展，企业不断壮大。在企业不断壮大发展的过程中，逐渐暴露出诸多问题，如生产计划制定缺乏数据支撑、没有完善的库存管理机制、产品质量无法跟踪和控制等。本文针对该公司的生产管理现状，结合软件技术、网络技术，探讨生产管理系统的开发方案，以期实现企业生产的信息化和自动化管理。 本文首先介绍了生产管理的国内外研究现状和课题研究...With the progress of computer and network technology, the major manufacturing enterprises has been widely and thoroughly carried out informatization construction work, by applying information technology to the procurement, production, processing and marketing, to improve the efficiency of enterprise production, aim to increase the competitiveness of the enterprise itself. Hangzhou cape magnetic in...学位：工程硕士院系专业：软件学院_软件工程学号：X201323176

A TWO-PARTICLE TURBIDIMETRIC LATEX IMMUNOASSAY FOR THE DETECTION OF SPECIFIC ANTIBODIES OF TRICHINELLA SPIRALIS

目的　建立旋毛虫病的快速检测技术。方法　基因工程抗原包被有色乳胶颗粒，抗抗体包被磁性颗粒，在抗体存在下形成抗原－ 乳胶－ 抗体－ 抗抗体－ 磁性颗粒的复合场，在磁场作用下沉淀下来，从而达到快速检测旋毛虫相关抗体的目的。结果　１９ 份人工感染鼠血清、５ 份人工感染猪血清、３ 份旋毛虫病猪血清、４ 份病人血清均呈阳性反应，而对照血清均为阴性反应；该方法在鼠感染旋毛虫后第五天可测出ＩｇＭ 抗体，第九天可测出ＩｇＧ抗体。结论　本检测技术简便易行，不需专用设备，可望成为一种快速诊断旋毛虫病的有效方法。Aim To establish a rapidly detecting method for the specific antibodies of Trichinella spiralis Method Latex was coated with p49/GST and polystyrene beads(Dynabeads)were coated with anti-antibodies SAM-IgG、SAH-IgG、RAP-IgG or SAM-IgM Then the test serum was incubated with two particles for 30 min at room temperature with slowly shaking After sedimentation of the polystyrene beads with a magnet,the turbidity of the resultant latex suspension was estimated with eyes or measured spectrophotometrically at a wavelength of 400nm Results Positive result of IgG antibody were found in 19 sera of experimental infected mice,3 sera of experimental infected swine and 4 sera of patients The IgM and IgG can be check up in mice after 5 and 9 days after experimental infection The result agreed with those obtained by ELISA Conclusion A two-particle turbidimetric latex immunoassy established by this study is a rapid,easy and precise method for the diagnosis of the trichinosis福建省科委(农医)项目!(95-Z150

DIAGNOSIS OF TRICHINOSIS BY ELISA WITH P49/GST ANTIGEN

目的以纯化的融合蛋白 p49/GST为抗原建立ELISA检测方法。方法对一批试验血清进行间接ELISA检测。结果 19份人工感染鼠血清、5份人工感染猪血清、4份旋毛虫病猪血清、4份病人血清呈IgG抗体阳性 ,2 1份人工感染鼠血清呈IgM抗体阳性 ,而正常对照血清及 30 0份屠宰场待检猪血清均呈阴性反应 ,其结果与常规压片法结果相符。结论融合蛋白p49/GST对于研制旋毛虫病的诊断抗原具有的潜在应用价值Aim To establish ELISA detecting method for the specific antibodies of Trichinella spiralis.Method A series of confirmed trichinosis sera were detected with ELISA with fusion protein p49/GST.Results Positive results of IgG antibody were found in 19sera of experimental infected mouse and 3sera of infected swine and 4 sera of patients;positive results of IgM antibody were found in 21 sera of experimental infected mouse .All normal sera were negative. Conclusion The ELISA with p49/GST can be regarded as a sensitive, specific immunological method for the diagnosis of trichinosis.福建省重点(农医 )项目资助!(项目号 :95 -Z -15 0

Construction of SSH library from haemocyte of variously colored abalone challenged with bacteria and differential expression analysis of macrophage expressed protein

以雌性杂色鲍为对象,以大肠杆菌、副溶血弧菌、溶壁微球菌、表皮葡萄球菌、金黄色葡萄球菌的混悬液做为攻毒菌,利用抑制性差减杂交(SSH)技术构建细菌攻毒的杂色鲍血淋巴细胞SSHcDNA文库。随机挑取生长菌落110个克隆子,进行菌液PCR鉴定,计算文库重组率为98.18%,文库容量为1.37×106pfu。将重组子测序,经BLAST一致性搜索比对分析,有一重组片段含有穿孔素(Perforin)保守结构域,为巨噬细胞表达蛋白(MEP)类穿孔素部分cDNA序列,片段大小为1551bp,连续编码517个氨基酸残基,申请GenBank登录号为EU272049。经半定量PCR和荧光定量PCR差异显示分析,发现在细菌感染状态下MEP基因在血淋巴细胞中存在明显的上调表达现象。Abalones are considered to be the most precious delicacy from the sea, and become very important commercial seafood in aquaculture worldwide. Variously colored abalone (Haliotis diversicolor Reeve, 1846) has been widely cultured on the southeast coast for more than twenty years. However, abalone culture frequently suffers from bacterial infection and mass mortality of reared abalones causes serious economic losses. Unfortunately, knowledge of the defense mechanism in this animal is still lacking. In this study, using suppression subtractive hybridization (SSH) technology, a forward SSH li-brary was constructed from haemocytes of H. diversicolor, with the content of 1.37×106 pfu and the recombinant rate of98.18%. After the recombinant plasmids were sequenced, partial cDNA of macrophage expressed protein (MEP) was recognized based on BLAST searches in NCBI, with the size of 1 551 bp, and continuously encoding 517 amino acids. Semi-quantitative PCR and quantitative real-time PCR results showed that MEP cDNA was distinctly up-regulated in haemocytes of the bacterial-challenged group compared to the unchallenged group. The gene information obtained from this library will provide new insights into the immune mechanism of H. diversicolor and facilitate future study of target genes involved in the response to invading microorganisms.国家高技术研究发展计划项目(863计划)(编号:2007AA091406)资助~

Cloning and Expression of the Leukotoxin Gene SH from Fusobacterium necrophorum

坏死梭杆菌白细胞毒素是坏死杆菌病的主要致病因子,白细胞毒素基因(lkT)是其编码基因。以分离到的国内牛源坏死梭杆菌fn(A)菌株f4基因组dnA为模板,应用PCr方法扩增白细胞毒素基因SH片段,克隆至PMd18-T载体上,以bAMHⅠ和HIndⅢ酶切的目的片段SH与相应酶切的PET32A载体连接构建PET32A-SH重组表达质粒,经转化E COlI bl21(dE3)后用IPTg进行蛋白诱导,SdS-PAgE检测重组蛋白表达情况。结果表明:扩增基因序列大小为1800bP,SdS-PAgE检测重组蛋白有效表达,表达得到大小为80.2kdA的目的蛋白,采用镍柱亲和层析方法纯化SH重组蛋白,获得了纯度达95%的重组蛋白;经WEST-Ern-blOT证实,该蛋白对抗坏死杆菌阳性血清具有反应活性。The leukotoxin of Fusobacterium necrophorum(FN) is considered to be one of the main virulence factors.The lkt gene encodes for FN.In this study,the SH fragment of lkt gene was amplified by PCR using the F4 genome as the template,which was isolated from the Chinese Fusobacterium necrophorum strain.The fragment was then cloned to the pMD18-T vector for sequencing.Thereafter,the SH fragment was subcloned into the multiple cloning sites of the pET32 to construct pET32a-SH recombinant plasmid,which was then trans-formed into E.coli BL21(DE3) with IPTG induction for expression.SDS-PAGE was used to analyze the recombinant protein.The results showed that the SH fragment of about 1800 bp was amplified and was about 80.2 kDa.The fusion protein was purified by Ni-NTA affinity chromatography under denature conditions,and their purity was above 95%.Western-blot analysis indicated the SH fragment had anti-genicity against Fusobacterium necrophorum.“十五”国家科技攻关子课题(2002BA518A04);吉林省科技发展计划项目(20070570);吉林市科技发展计划项目(200805

Optimization of Medium for Deacetylmycoepoxydiene Fermentation by Response-surface Methodology

从海洋真菌PHOMOPSIS SP.A123分离得到的南强菌素(dEACETylMyCOEPOXydIEnE)是2003年新发现的具有抗肿瘤活性化合物.采用单因素筛选方法,筛选得到影响该化合物产生的4个因素,分别为葡萄糖、维生素b1、马铃薯和甘露醇.在此基础上,采用响应面法对4个因素最佳水平范围进行研究,利用dESIgn-EXPErT软件进行2次回归分析,在培养基中葡萄糖、维生素b1、马铃薯和甘露醇的含量分别为87,0.005,170和14 g/l时,南强菌素的产量从35 Mg/l提高到200 Mg/l.Deacetylmycoepoxydiene(DAM),isolated from the marine fungus Phomopsis sp.A123,is a novel antitumor compound.It was first isolated in 2003.In the present work,after single-factor optimization,four key factors with strong impact on the production of DAM,namely,glucose,potato,vitamin B1 and mannitol were selected.The centre composition design of response surface method was applied to optimize the best concentration of the four factors.The optimization levels of the various ingredients were:glucose 87 g/L,potato 170 g/L,vitamin B1 5 mg/L and mannitol 14 g/L,and the predicted yield of DAM in liquid medium was 220 mg/L.After applying the quadratic regression model equation,the actual yield of DAM reached 200 mg/L,this was 6 times that of the initial medium.国家863计划项目(2007AA091503)资

实验设计法优化高浓度静注人免疫球蛋白制剂的处方

目的通过实验设计法优化高浓度(10%)静注人免疫球蛋白(human immunoglobulin for intravenous injection, IVIG)的制剂处方。方法采用单因素试验(包括等电点、二级结构、分子大小分布,转变中点温度4个指标)确定IVIG(10%)的pH范围,同时经国外相关数据和产品质量指标选择IVIG的稳定剂甘氨酸和聚山梨酯80的浓度范围,再采用实验设计法优化IVIG的pH及稳定剂浓度。以转变中点温度为指标,通过单因素试验优化IVIG(10%)的离子强度。结果最佳IVIG(10%)制剂处方为聚山梨酯80:80 mg / L,pH:4.5,甘氨酸:0.25 mol / L。最适离子强度为0 mmol / L氯化钠。结论成功优化了IVIG(10%)的制剂处方,可确保制剂的稳定性,提高临床用药安全性

Preparation of Deacetylmycoepoxydiene with Reversed Phase Chromatography

研究不同条件下反相柱层析对去乙酰真菌环氧乙酯分离纯化的影响.通过rP-18中压柱层析对从红树林内生真菌拟茎点霉(PHOMOPSISSP.)A-1-2-3诱变后突变株g1-1固体发酵提取物中的去乙酰真菌环氧乙酯进行了纯化精制.对rP-18柱层析纯化去乙酰真菌环氧乙酯操作条件进行了考察,使去乙酰真菌环氧乙酯的含量从28%左右提高到81.5%左右,回收率大于90%.采用rP-18直接洗脱,溶析结晶,重结晶,结晶残液rP-18分步洗脱的总工艺路线,去乙酰真菌环氧乙酯的总回收率为87.7%,产品最终纯度达到99%以上.The effects on purification of deacetylmycoepoxydiene(DAM) in different conditions were studied.Preparation of DAM from the crude extract of fermentation of strain G1-1,a mutant strain of mangrove endophytic phomopsis sp.A-1-2-3,was conducted using C18-silica gel reversed phase chromatography at moderate pressure.In the process,the operating conditions of C18-silica gel chromatography were studied experimentally,then the purity of DAM reached about 81.5% from about 28% in the crude extract,and the recovery of DAM was over 90%.The whole process of preparation was as follow: direct elution with RP-18,solvent-out crystallization,recrystallization and segment elution of residue of crystallization with RP-18.Through the whole process flow,the general recovery of DAM was 87.7%,and the purity of DAM reached 99% or higher.国家海洋863项目(2007AA091503)资