Technolog of extraction and preparation of fraction of small molecule metabolites from marine microbe

本论文主要是以海洋微生物发酵液为研究对象，探索海洋微生物小分子代谢产物高效率、高通量的提取和制备分离方法，建立海洋微生物小分子代谢产物馏分库以供活性药物的初步筛选，为最终建立海洋微生物小分子代谢产物活性化合物库奠定基础。具体内容如下： 第一：为提高过滤速率，对海洋细菌和放线菌发酵液进行预处理研究，主要以海洋细菌发酵液为研究对象，采用絮凝技术对发酵液进行预处理。以610nm透光率、蛋白去除率、多糖去除率、过滤速度等为指标，从8种不同类型的絮凝剂中筛选出适合的絮凝剂，通过HPLC分析比较了两种絮凝剂对发酵液中小分子代谢产物提取的影响，并探讨了所筛选絮凝剂的使用条件。比较了4种固液分离方法对细菌发...A high throughput extraction and preparative separation technology was studied in this thesis. The purpose of this work is to establish small molecule metabolites fraction libraries for preliminary screening of bioactive substances. This work creates the foundation for building a library of active small molecule metabolites from marine microorganisms. More details are as follows: Firstly, to impr...学位：理学硕士院系专业：生命科学学院_微生物学学号：2162011115237

Studies on extraction of small molecule metabolites produced by marine microorganisms with macroporous adsorption resin

目的采用大孔吸附树脂建立海洋微生物小分子代谢产物库的粗提取方法。方法以吸附率、解吸率、蛋白与总糖吸附率、指纹图谱为评价目标,对常用的11种不同极性的大孔吸附树脂进行筛选。结果 dM11与Sd300串联吸附对发酵液中化合物的吸附率达7.528%,总糖与蛋白去除率分别为90.98%和77.69%,且此法几乎不损失乙酸乙酯萃取部分化合物。结论 dM11-Sd300串联树脂吸附法能快速有效地提取发酵液中的小分子化合物,为海洋微生物小分子代谢产物库的建立提供了实验基础。OBJECTIVE To establish a crude extraction method for the library of small molecule metabolites produced by marine microorganisms using macroporous adsorption resin( MAR).METHEDS 11 types of MARs were selected based on their adsorption rate,desorption rate,protein and polysaccharide adsorption rate.RESULTS DM11 and SD300 in series,were used to extrat small chemical molecules from bacterial fermentation liquid,by which the adsorption rate is up to 7.528%,while the removal rate of protein and polysaccharides is 90.98% and 77.69% respectively.In addition,this process barely lose compounds that extracted from ethyl acetate.CONCLUSION the approach of DM11-SD300 in series could extract small molecule metabolites from fermentation liquid rapidly and effectively,which will make a fundation to achieve the library of small molecule metabolites produced by marine microorganisms

Technolog for rapidly pretreating fermentation borth of marine bacterias

以610 nM透光率、蛋白去除率、多糖去除率、过滤速度等为指标,从8种不同类型的絮凝剂中筛选出适合的絮凝剂,通过HPlC分析比较了2种絮凝剂对发酵液中小分子代谢产物提取的影响,并探讨了所筛选絮凝剂的使用条件。比较了4种固液分离方法对细菌发酵液的分离效果,进而确定了适合海洋细菌发酵液快速预处理的工艺。结果表明:Al2(SO4)3耦合膜超滤法絮凝速度快、蛋白多糖去除率高、滤液澄清度好,适用于大部分海洋细菌发酵液的预处理。The optimum flocculation were screened from the different kinds of flocculations through comparison of610nm light-transmittance,the filtration rate,the removal ratio of protein and polysaccharose.Using Al2( SO4)3and poly-AlCl3 as flocculation,their effects on the extraction of the secondary metabolites from marine bacterias were investigated by HPLC( high-performance-liquid-chromatograhy).The effects of four kinds of solid-liquid separation ways on the fermentation borth of marine bacteria were compared.Results showed that the coupling method of flocculation and membrane ultrafiltration was adaptable to highly active and rapid pretreatment of different types of marine bacteria.国家海洋公益性行业专项(2010050225022); 中国大洋矿产资源研究开发协会专项(DY125-15-T-01

Electochemical Measuments of Defective Coatingson Buried Steel Substrates

基于电化学阻抗谱技术建立了实验室检测钢铁表面防护层破损与剥离的电化学方法．提出了在一定频率范围内以电极阻抗幅值比Ｋ的变化关系作为防护层缺陷状态的表征．用此方法测定了土壤中阴极保护的表面涂覆不同状态石油沥青防护层的Ａ３钢模拟试片，得到了较为满意的结果An electrochemical technique to detect the defects and detachments of the protective coating on the steel structures with electrochemical impedance spectroscopy (EIS) was offered. A new parameter K,which was defined as the ratio of the electrode resistance amplitude at a certain frequency to that at minimal frequency was obtained in present paper. The results indicated that the change of K at a certain range of frequency reflected the condition of protective coating.The technique was found to be very useful in estimating the different conditions of protective coating after being applied to the carbon steel samples covered with tar asphalt which were buried in soil and subjected to cathodic protection.作者联系地址：天津大学,中国石油天然气管道科学研究院Author's Address: Tianjin UniversityTianjin300072 Liao YupingLai GuangsenLiu Lingli China Oil & Gas Pipeline Scientific Research InstituteLangfang06500

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies