东方肉穗草异鼠李素诱导HepG2细胞凋亡研究

目的研究东方肉穗草中的主要抗癌活性成分之一异鼠李素诱导HepG2细胞凋亡的机理。方法采用MTT法检测异鼠李素对肝癌HepG2细胞的抑制作用,DAPI染色法对细胞凋亡进行形态学检测。结果实验结果显示异鼠李素对肝癌HepG2细胞的增殖有明显抑制作用,呈浓度依赖性效应。异鼠李素处理48h的IC50为60μmol/L。荧光显微形态学检测显示异鼠李素处理HepG2细胞后出现细胞凋亡的特征性变化。结论研究表明东方肉穗草异鼠李素可以诱导肝癌HepG2细胞凋亡

东方肉穗草黄酮类化学成分研究

目的:系统研究东方肉穗草中的黄酮类化学成分。方法:利用大孔吸附树脂、Sephadex LH-20,ODS及正相硅胶柱等色谱手段进行分离,通过多种波谱学数据分析进行黄酮类化合物的结构鉴定。结果:从醋酸乙酯部分分离得到8个黄酮类化合物,经鉴定为异鼠李素(1)、槲皮素(2)、异鼠李素-3-O-β-D-吡喃葡萄糖苷(3)、槲皮素-3-O-β-D-葡萄糖苷(4)、异鼠李素-3-D-(6″-乙酰基)-β-D-吡喃葡萄糖苷(5)、异鼠李素-3-O-(2″-乙酰基)-β-D-吡喃葡萄糖苷(6)、槲皮素-3-O- (6″-乙酰基)-β-D-吡喃葡萄糖苷(7)、槲皮素-3-O-(6″-反式-对香豆酰基)-β-D-吡喃葡萄糖苷(8)。结论:所有化合物均系首次从该属植物中分离鉴定

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies