Study on the Effects and Mechanisms of Recombinant Human Prothymosin α in Metabolism of Glucose and Lipid

目前，人们正越来越多地食用高糖高脂食品。大量临床研究表明，长期食用高糖高脂食品将引起肥胖症，而肥胖将导致机体糖耐量作用下降，引发胰岛素抵抗，最终诱发2型糖尿病。更为严重的是，糖尿病将引发如酮症酸中毒、脑血管疾病、肾病等其它并发症。如何预防和治疗糖尿病及其并发症已经成为医学界关注的焦点。糖尿病的基本特征是胰岛β细胞的结构和功能受损，导致机体胰岛素分泌不足。依赖外供给胰岛素是目前主要的治疗方案，然而通过防止胰岛β细胞凋亡和促进胰岛β细胞分裂的角度进行研究，将是预防和治疗糖尿病的新途径。 经研究证实，胸腺素α原（ProTα）具有促进机体免疫功能，促进细胞增殖和抗氧化功能的作用。由于ProTα的特殊...High sucrose and high fat diet (HSFC diet) is more and more popular in the world. There are a lot of evidence indicated that a long term intake HSFC diet will lead overweight obesis that usually will cause glucose intolerant,insulin resistance and eventually result in type 2 diabetes. Even worse, diabetes will cause ketoacidosis, cerebrovascular disease, diabetic nephropathy and other syndromes. H...学位：理学硕士院系专业：生命科学学院生物学系_动物学学号：2162007115199

Experimental Study on Effects of Hypoglycemic and Antiobesity of L-Arabinose in New Zealand Rabbit

目的探讨l-阿拉伯糖对动物(新西兰兔)降血糖和减肥的作用。方法以高糖高脂饲料诱导新西兰兔肥胖模型,然后将其随机分为4组:模型对照组及l-阿拉伯糖低剂量(0.3085g/kg)、中剂量(0.617g/kg)、高剂量(0.9255g/kg)3个给药组。连续喂养2个月后,空腹12H,各给药组按剂量口服l-阿拉伯糖后2H,分别口服蔗糖0、0.5、2H测新西兰兔耳缘静脉血糖值;l-阿拉伯糖干预1个月时测体重、进食量和排便量、脂肪指数的变化。结果高、中、低l-阿拉伯糖组空腹血糖与模型对照组存在明显差异(P<0.05),在糖耐量作用中,l-阿拉伯糖各剂量组和模型对照组的曲线下面积(AuC)出现明显差别。在减肥功能的指标中(体重变化、日进食量和排便量、脂肪指数),l-阿拉伯糖各剂量组与模型对照组比较差异有统计学意义(P<0.05),其中l-阿拉伯糖组与模型对照组的脂肪指数具有显著差异(P<0.01)。结论l-阿拉伯糖有明显降血糖和减肥的作用。Objective To research the function of hypoglycemic and antiobesity of L-Arabinose in New Zealand rabbit.Methods Thirty-two New Zealand white rabbits were randomly divided into 4 groups, 8 rabbits in each group.The control group was fed with high fat and high sucrose diet only.L-Arabinose group was fed with high fat and high sucrose diet along with L-Arabinose, the dosage were 0.308 5, 0.617, 0.925 5 g/kg respectively.After fed for two months, all groups were given L-Arabinose, and blood glucose was detected after orally given sucrose 0, 0.5, 2 h.After intervention of L-Arabinose for one month, the data of weight, food intake, stool quantity and the fat index were observed.Results L-Arabinose decreased significantly sucrose absorption, lower the fasting glucose level and the data of area-under-curve (AUC) significantly.Conclusion L-Arabinose proved to be highly effective in preventing the rise of circulating glucose and fat

Study on the prothymosin α as vaccine adjuvant of P.yoelii

目的:研究胸腺素α原(PrOTHyMOSInα,PrOTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yOElII-17Xnl全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yOElII-17Xnl全蛋白+PrOTα;b组免疫P.yOElII-17Xnl全蛋白;C组只注射PrOTα;d组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yOElII-17Xl,1x107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,b、d组小鼠全部死亡。结论:用P.yOElII-17Xnl全蛋白做抗原,用PrOTα作为佐剂,比单独用P.yOElII-17Xnl全蛋白对小鼠有更好的免疫保护作用,提示了PrOTα可以成为一种有潜力的蛋白疫苗。Objective:To investigate the function of prothymosin α(ProTα) as vaccine adjuvant of P.yoelii.Methods:The mice were immunized with the total protein extracted from P.yoelii-17XNL as antigen,together with prothymosin α as adjuvant.Programs:Kunming mice were divided into A,B,C and D group.A group was immunized with P.yoelii-17XNL total protein and ProTα;B group was immunized with P.yoelii-17XNL;C group was only injected with ProTα;D group was the control,only injected with physiological saline.And then,the mice of each group was infected with P.yoelii-17XL,the dosage was 1×107/mice.Results:The parasitemia of A group-mice was lower than the other three groups in the first 10 days after infection,and eventually there were three mice survived in A group,the survival rate was 50%,one mouse survived in C group,all of mice in B group and D group died.Conclusion:Mice immunized with P.yoelii-17XNL total protein as antigen together with ProTα as adjuvants,had better immune protection than those immunized with P.yoelii-17XNL protein only.The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103)资

Effects of L-Arabinose as Inhibitor on Body Mass and Sucrose Tolerance in Mice with High Fat and High Sucrose Fedding

L-阿拉伯糖作为一种低能量的甜料,能抑制蔗糖水解酶的活性,从而抑制蔗糖的吸收.L-阿拉伯糖作为新资源食品已经通过国家卫生部的批准.本研究的目的是探讨唐传生物科技(厦门)有限公司从水稻,玉米秸秆中提取的L-阿拉伯糖在降低空腹血糖,提高糖耐量及控制体质量方面的重要生物学功能,从而为其功能食品的进一步开发提供科学依据.以20～22 g昆明小鼠为实验动物,在给予高糖高脂饲料喂养的同时,采用高、中、低3种剂量口服L-阿拉伯糖水溶液1个月,以单独喂养高糖高脂饲料喂养组为对照,实验期间检测小鼠体质量变化和食量.4周结束后进行小鼠空腹血糖及蔗糖糖耐量试验.结果显示高剂量组(250 mg/只)和中剂量组(150 mg/只)对小鼠空腹血糖有明显降低作用(p<0.05),而低剂量组(30 mg/只)作用不明显,糖耐量试验证明3个剂量组的L-阿拉伯糖都具有明显增强小鼠糖耐量作用,与对照组有极显著差异(p<0.01),实验过程中未发现实验组与对照组小鼠食量有明显差异.实验结果表明,L-阿拉伯糖能够明显降低喂养高糖高脂饲料小鼠的空腹血糖,增强糖耐量,并且能够有效减缓体质量增长趋势.L-Arabinose is a sweetener in low-energy,it can inhibit the activity of enzymes,as a natural enzymy inhibitor of sucrase,lessen breakdown of sucrose,limited its absorption in the gatrointestinal tract.L-Arabinose has been approved as a new food resources by Ministry of Health of P.R.China.The result will help to eliminate the rising of circulation blood glucose level after the sucrose challenge,the inhibiting the absorption of sugar can prevent hypertension and diabetes.Thomson biotech(Xiamen)PTE LTD extract the L-Arabinose from rice,corn stalks with biochemical methods.The aim of this study is to examine the biological activity of L-Arabinose from the company and provide experimental basis to further the development of functional foods.100 four-week male Kunming mice were randomly divided into 4 groups,A,B,C,D group,20 mice one group,D group fed with high fat and high sucrose diet only as control group,A,B,C group along with high fat and high sucrose diet,oral with L-Arabinose,the dosage were 250,150 and 30 mg/d respectively for 4 weeks.The body weight and the amount of food intake were monitored every 5 day and every 2 day,respectively,for 4 weeks,the fasting glucose,sucrose tolerance were examined using commercial glucose strips(Lifescan,One Touch Ultra,Melitas,CA).The experimental result proved that the L-Arabinose decreased significantly sucrose absorption,lower the fasting glucose level and the data of aera-under-curve(AUC)significantly in the mice with high fat and high sucrose fedding.The high dosage and middle dosage group,the L-Arabinose can decrease the weight increse,These results support the hypothesis that the L-Arabinose preparation by Thomson biotech(Xiamen)PTE LTD proved to be highly effective in preventing a rise in circulating glucose after sucrose challenge.The body mass gain was less in the L-Arabinose treated group from 5th day of than that in the control group,the AUC data were significantly lower in L-Arabinose group than that of control group.福建省化学生物重点实验室开放课题基金(0660,K70012)资